Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,214	G→T	A55S (GCC→TCC)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,214	0	G	T	100.0%	149.7 / NA	53	A55S (GCC→TCC)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/29);  total (24/29)

AAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTC  >  1/1482989‑1483454                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aaaaaCAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACTCTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAAt                                                                                                                                                                                                                         <  1:73968/251‑1 (MQ=255)          gTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCtga                                                                                                                                                                                                                >  1:62286/1‑251 (MQ=255)                            ttacAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                                                                                                                                              <  1:61077/248‑1 (MQ=255)                                     tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAg                                                                                                                                                                                                      <  1:391690/234‑1 (MQ=255)                                     tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACg                                                                                                                                                                                     >  1:646085/1‑251 (MQ=255)                                             caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                                                                                                                                             >  1:123341/8‑250 (MQ=255)                                             caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                                                                                                                                             >  1:386819/8‑250 (MQ=255)                                                    tAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTg                                                                                                                                                                       <  1:551862/250‑1 (MQ=255)                                                       tggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTg                                                                                                                                                                                                        >  1:330389/5‑214 (MQ=255)                                                        ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGAt                                                                                                                                                                                                          <  1:71867/208‑1 (MQ=255)                                                        ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGt                                                                                                                                                                                                   >  1:612660/4‑218 (MQ=255)                                                        ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                                                                                                                                              >  1:879319/4‑223 (MQ=255)                                                        ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                                                                                                                                  >  1:367179/4‑251 (MQ=255)                                                        ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                                                                                                                                  >  1:42600/4‑251 (MQ=255)                                                         gttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                                                                                                                                  <  1:167461/248‑1 (MQ=255)                                                                aTGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATaaa                                                                                                                                                          <  1:722872/251‑1 (MQ=255)                                                                   cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                                                                                                                        <  1:764746/250‑1 (MQ=255)                                                                   cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                                                                                                                        <  1:328925/250‑1 (MQ=255)                                                                   cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCAATAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                                                                                                                        <  1:184408/250‑1 (MQ=255)                                                                      aCCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGatat                                                                                                                                                    <  1:8576/251‑1 (MQ=255)                                                                             aacaaAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGtt                                                                                                                                             <  1:536723/251‑1 (MQ=255)                                                                                           cttaGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa                                                                                                                               <  1:840605/251‑1 (MQ=255)                                                                                                  gtgaagtATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa                                                                                                                               <  1:152822/244‑1 (MQ=255)                                                                                                       gtattgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGa                                                                                                                   >  1:676058/10‑251 (MQ=255)                                                                                                       gtattgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGa                                                                                                                   >  1:590920/10‑251 (MQ=255)                                                                                                         attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGGCGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc                                                                                                                 <  1:36229/244‑1 (MQ=255)                                                                                                         attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc                                                                                                                 <  1:860441/244‑1 (MQ=255)                                                                                                         attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc                                                                                                                 <  1:177570/244‑1 (MQ=255)                                                                                                            gagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGt                                                                                                                      >  1:188727/5‑243 (MQ=255)                                                                                                                 aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACggga                                                                                                                                                                                  <  1:198914/178‑4 (MQ=255)                                                                                                                 aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                                                                                                                        <  1:221242/204‑1 (MQ=255)                                                                                                                 aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGat                                                                                                                                                      <  1:496865/206‑1 (MQ=255)                                                                                                                 aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGat                                                                                                                                                      >  1:176770/1‑206 (MQ=255)                                                                                                                 aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATg                                                                                                                                >  1:816649/1‑228 (MQ=255)                                                                                                                     tGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTc                                                                                                     <  1:211039/251‑1 (MQ=255)                                                                                                                                                aaGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGtt                                                                                                                                             >  1:308875/1‑184 (MQ=255)                                                                                                                                                  gTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATc                                                                                                                           >  1:264397/1‑200 (MQ=255)                                                                                                                                                  gTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTc                                                                                                     <  1:23339/222‑1 (MQ=255)                                                                                                                                                           gctcattagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTg                                                                                                                         <  1:645237/185‑1 (MQ=255)                                                                                                                                                           gctcattagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAAc                                                                 >  1:378392/9‑249 (MQ=255)                                                                                                                                                                ttagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGt                                                                                                         <  1:473317/201‑1 (MQ=255)                                                                                                                                                                ttagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTaa                                                           >  1:719901/4‑250 (MQ=255)                                                                                                                                                                    tAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAGTTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACAGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGa                                                       <  1:91403/250‑1 (MQ=255)                                                                                                                                                                    tAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGat                                                      >  1:203429/1‑251 (MQ=255)                                                                                                                                                                          aaCTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCa                                                >  1:865880/1‑251 (MQ=255)                                                                                                                                                                                    gATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGc                                                                                                                                       <  1:547307/154‑1 (MQ=255)                                                                                                                                                                                    gATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACt                                                         <  1:338096/232‑1 (MQ=255)                                                                                                                                                                                                       aataaaagtaacacTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCgtcg                   >  1:15157/14‑248 (MQ=255)                                                                                                                                                                                                                  cacTTATTTGACGTCATCCGATGCGCTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcgtg                                                                                                                                                                           <  1:205922/86‑4 (MQ=255)                                                                                                                                                                                                                  cacTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGAc                                                                                                                                                                     >  1:683125/3‑94 (MQ=255)                                                                                                                                                                                                                      tATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg           >  1:835611/1‑244 (MQ=255)                                                                                                                                                                                                                      tATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg           >  1:287448/1‑244 (MQ=255)                                                                                                                                                                                                                        tttGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTc  <  1:865849/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTC  >  1/1482989‑1483454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑