Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,150	G→A	L33L (CTG→TTA)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,150	0	G	A	100.0%	145.8 / NA	55	L33L (CTG→TTA)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/33);  total (22/33)

TAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTC  >  1/1482921‑1483355                                                                                                                                                                                                                                      |                                                                                                                                                                                                              gaaaaaGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGAt                                                                                                                                                                                           <  1:539729/249‑1 (MQ=255)          aattaattCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                                                 >  1:175824/1‑251 (MQ=255)              aattCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTg                                                                                                                                                                             <  1:763224/251‑1 (MQ=255)                  cactAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCaa                                                                                                                                                                         >  1:739751/4‑251 (MQ=255)                                gTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                                                 <  1:466703/229‑1 (MQ=255)                                gTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacactt                                                                                                                                                           >  1:61538/1‑243 (MQ=255)                                          cAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacacttatttgacgt                                                                                                                                                  <  1:214262/250‑18 (MQ=255)                                              atCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                                                 >  1:65563/1‑215 (MQ=255)                                              atCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacacttatttgacgtcatc                                                                                                                                              >  1:500580/1‑229 (MQ=255)                                                                     aaaaaCAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACTCTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAAt                                                                                                                      <  1:73968/251‑1 (MQ=255)                                                                              gTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCtga                                                                                                             >  1:62286/1‑251 (MQ=255)                                                                                                ttacAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGtt                                                                                                                                                                                                <  1:488547/147‑1 (MQ=255)                                                                                                ttacAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                                           <  1:61077/248‑1 (MQ=255)                                                                                                         tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgta                                                                                                                                                                                <  1:45016/157‑2 (MQ=255)                                                                                                         tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAg                                                                                                   <  1:391690/234‑1 (MQ=255)                                                                                                         tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACg                                                                                  >  1:646085/1‑251 (MQ=255)                                                                                                          cATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAg                                                                                                                                                                   <  1:452402/169‑1 (MQ=255)                                                                                                                acaataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGAtt                                                                                                                                                                                          <  1:628842/132‑1 (MQ=255)                                                                                                                 caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                                          >  1:386819/8‑250 (MQ=255)                                                                                                                 caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                                          >  1:123341/8‑250 (MQ=255)                                                                                                                        tAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTg                                                                    <  1:551862/250‑1 (MQ=255)                                                                                                                           tggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTg                                                                                                     >  1:330389/5‑214 (MQ=255)                                                                                                                            ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGAt                                                                                                       <  1:71867/208‑1 (MQ=255)                                                                                                                            ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGt                                                                                                >  1:612660/4‑218 (MQ=255)                                                                                                                            ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                                           >  1:879319/4‑223 (MQ=255)                                                                                                                            ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                               >  1:367179/4‑251 (MQ=255)                                                                                                                            ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                               >  1:42600/4‑251 (MQ=255)                                                                                                                             gttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                                               <  1:167461/248‑1 (MQ=255)                                                                                                                                   cATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTaatc                                                                                                                                                                                   >  1:414397/1‑126 (MQ=255)                                                                                                                                    aTGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATaaa                                                       <  1:722872/251‑1 (MQ=255)                                                                                                                                       cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                     <  1:764746/250‑1 (MQ=255)                                                                                                                                       cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                     <  1:328925/250‑1 (MQ=255)                                                                                                                                       cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCAATAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                     <  1:184408/250‑1 (MQ=255)                                                                                                                                         gACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                                                 <  1:58/124‑1 (MQ=255)                                                                                                                                          aCCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGatat                                                 <  1:8576/251‑1 (MQ=255)                                                                                                                                                 aacaaAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGtt                                          <  1:536723/251‑1 (MQ=255)                                                                                                                                                  acaaAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                                                 <  1:52017/115‑1 (MQ=255)                                                                                                                                                               cttaGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa                            <  1:840605/251‑1 (MQ=255)                                                                                                                                                                      gtgaagtATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa                            <  1:152822/244‑1 (MQ=255)                                                                                                                                                                           gtattgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGa                >  1:590920/10‑251 (MQ=255)                                                                                                                                                                           gtattgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGa                >  1:676058/10‑251 (MQ=255)                                                                                                                                                                             attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGGCGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc              <  1:36229/244‑1 (MQ=255)                                                                                                                                                                             attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc              <  1:177570/244‑1 (MQ=255)                                                                                                                                                                             attgagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAAc              <  1:860441/244‑1 (MQ=255)                                                                                                                                                                                gagcaaTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGt                   >  1:188727/5‑243 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATaaa                                                                                                                                                                     <  1:511869/92‑1 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACggga                                                                               <  1:198914/178‑4 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                                                     <  1:221242/204‑1 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGat                                                   <  1:496865/206‑1 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGat                                                   >  1:176770/1‑206 (MQ=255)                                                                                                                                                                                     aTCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATg                             >  1:816649/1‑228 (MQ=255)                                                                                                                                                                                         tGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTc  <  1:211039/251‑1 (MQ=255)                                                                                                                                                                                                                    aaGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGtt                                          >  1:308875/1‑184 (MQ=255)                                                                                                                                                                                                                      gTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATc                        >  1:264397/1‑200 (MQ=255)                                                                                                                                                                                                                      gTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTc  <  1:23339/222‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                              TAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTC  >  1/1482921‑1483355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑