Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,094	C→T	L15M (CTA→TTG)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,094	0	C	T	100.0%	138.4 / NA	52	L15M (CTA→TTG)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

ATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGA  >  1/1482868‑1483329                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            gttAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGa                                                                                                                                                                                                                     >  1:106686/2‑251 (MQ=255)                       ctgtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaag                                                                                                                                                                                               >  1:345357/12‑251 (MQ=255)                         gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa                                                                                                                                                                                             >  1:116083/10‑251 (MQ=255)                         gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa                                                                                                                                                                                             >  1:678648/10‑251 (MQ=255)                         gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa                                                                                                                                                                                             >  1:515766/10‑251 (MQ=255)                         gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa                                                                                                                                                                                             >  1:484265/10‑251 (MQ=255)                                gcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGatg                                                                                                                                                                                                                              <  1:529389/209‑2 (MQ=255)                                 caTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACACCAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCatta                                                                                                                                                                                     >  1:718472/2‑248 (MQ=255)                                     aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCattagtaa                                                                                                                                                                                 >  1:328079/1‑244 (MQ=255)                                                      gaaaaaGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGAt                                                                                                                                                                 <  1:539729/249‑1 (MQ=255)                                                               aattaattCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                       >  1:175824/1‑251 (MQ=255)                                                                   aattCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTg                                                                                                                                                   <  1:763224/251‑1 (MQ=255)                                                                       cactAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCaa                                                                                                                                               >  1:739751/4‑251 (MQ=255)                                                                                    ggTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGatg                                                                                                                                                                                                                              <  1:412526/159‑2 (MQ=255)                                                                                     gTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                       <  1:466703/229‑1 (MQ=255)                                                                                     gTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacactt                                                                                                                                 >  1:61538/1‑243 (MQ=255)                                                                                               cAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacacttatttgacgt                                                                                                                        <  1:214262/250‑18 (MQ=255)                                                                                                   atCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                       >  1:65563/1‑215 (MQ=255)                                                                                                   atCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAgtaacacttatttgacgtcatc                                                                                                                    >  1:500580/1‑229 (MQ=255)                                                                                                                          aaaaaCAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACTCTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAAt                                                                                            <  1:73968/251‑1 (MQ=255)                                                                                                                                   gTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCtga                                                                                   >  1:62286/1‑251 (MQ=255)                                                                                                                                       aTTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGatgt                                                                                                                                                                                                                             <  1:575132/109‑3 (MQ=255)                                                                                                                                                     ttacAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGtt                                                                                                                                                                      <  1:488547/147‑1 (MQ=255)                                                                                                                                                     ttacAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                 <  1:61077/248‑1 (MQ=255)                                                                                                                                                              tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgta                                                                                                                                                      <  1:45016/157‑2 (MQ=255)                                                                                                                                                              tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAg                                                                         <  1:391690/234‑1 (MQ=255)                                                                                                                                                              tCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACg                                                        >  1:646085/1‑251 (MQ=255)                                                                                                                                                               cATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAg                                                                                                                                         <  1:452402/169‑1 (MQ=255)                                                                                                                                                                     acaataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGAtt                                                                                                                                                                <  1:628842/132‑1 (MQ=255)                                                                                                                                                                      caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCattag                                                                                                                                                                                    <  1:244210/112‑5 (MQ=255)                                                                                                                                                                      caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                >  1:386819/8‑250 (MQ=255)                                                                                                                                                                      caataagtAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGAtctcg                                                >  1:123341/8‑250 (MQ=255)                                                                                                                                                                             tAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTg                                          <  1:551862/250‑1 (MQ=255)                                                                                                                                                                                tggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTg                                                                           >  1:330389/5‑214 (MQ=255)                                                                                                                                                                                 ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGAt                                                                             <  1:71867/208‑1 (MQ=255)                                                                                                                                                                                 ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGt                                                                      >  1:612660/4‑218 (MQ=255)                                                                                                                                                                                 ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATgg                                                                 >  1:879319/4‑223 (MQ=255)                                                                                                                                                                                 ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                     >  1:42600/4‑251 (MQ=255)                                                                                                                                                                                 ggttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                     >  1:367179/4‑251 (MQ=255)                                                                                                                                                                                  gttAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGAt                                     <  1:167461/248‑1 (MQ=255)                                                                                                                                                                                        cATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTaatc                                                                                                                                                         >  1:414397/1‑126 (MQ=255)                                                                                                                                                                                         aTGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATaaa                             <  1:722872/251‑1 (MQ=255)                                                                                                                                                                                            cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                           <  1:328925/250‑1 (MQ=255)                                                                                                                                                                                            cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                           <  1:764746/250‑1 (MQ=255)                                                                                                                                                                                            cTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCAATAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATg                           <  1:184408/250‑1 (MQ=255)                                                                                                                                                                                              gACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                       <  1:58/124‑1 (MQ=255)                                                                                                                                                                                               aCCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGatat                       <  1:8576/251‑1 (MQ=255)                                                                                                                                                                                                      aacaaAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGtt                <  1:536723/251‑1 (MQ=255)                                                                                                                                                                                                       acaaAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCgt                                                                                                                                                       <  1:52017/115‑1 (MQ=255)                                                                                                                                                                                                                   acttaGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGc                                                                                                                                                                                           >  1:513837/1‑67 (MQ=255)                                                                                                                                                                                                                    cttaGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa  <  1:840605/251‑1 (MQ=255)                                                                                                                                                                                                                           gtgaagtATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCATTAGTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGa  <  1:152822/244‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            ATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGA  >  1/1482868‑1483329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑