Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,921	T→G	G336G (GGT→GGG)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,921	0	T	G	100.0%	85.9 / NA	31	G336G (GGT→GGG)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/15);  total (16/15)

ATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAA  >  1/1482733‑1483154                                                                                                                                                                                             |                                                                                                                                                                                                                                          attcgcagtaCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGcc                                                                                                                                                                             <  1:883817/242‑1 (MQ=255)          aCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGaaaa                                                                                                                                                                    >  1:63248/1‑251 (MQ=255)                                     gtatGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                                                                                                                                              <  1:858971/211‑1 (MQ=255)                                       atGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                                                                                                                                             <  1:19406/196‑1 (MQ=255)                                               cTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCAtt                                                                                                                               >  1:171135/1‑251 (MQ=255)                                                   taatcggttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                                                                                                                           <  1:506710/237‑1 (MQ=255)                                                          ttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATaagt                                                                                                                    <  1:231075/244‑3 (MQ=255)                                                             gaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                                                                                                                           >  1:847208/5‑241 (MQ=255)                                                                      aaGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACa                                                                                                        <  1:591750/251‑1 (MQ=255)                                                                         ttAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACAt                                                                                                       <  1:256312/249‑1 (MQ=255)                                                                                                  ccttAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                                                                                                                                             <  1:393820/136‑1 (MQ=255)                                                                                                           gTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATc                                                                                 <  1:402244/237‑1 (MQ=255)                                                                                                           gTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAgaagtga                                                                   >  1:44448/1‑251 (MQ=255)                                                                                                              cttcAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAGCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAgaagtgaagt                                                                >  1:577410/1‑251 (MQ=255)                                                                                                              cttcAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                                                                                                                                              >  1:184587/1‑141 (MQ=255)                                                                                                                                        gttAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTattgagc                                                         <  1:476089/231‑7 (MQ=255)                                                                                                                                        gttAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGa                                      >  1:106686/2‑251 (MQ=255)                                                                                                                                                       aCAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCAttttttac                                                                                                                                         <  1:843045/137‑3 (MQ=255)                                                                                                                                                              ctgtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaag                >  1:345357/12‑251 (MQ=255)                                                                                                                                                                gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa              >  1:678648/10‑251 (MQ=255)                                                                                                                                                                gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa              >  1:116083/10‑251 (MQ=255)                                                                                                                                                                gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa              >  1:515766/10‑251 (MQ=255)                                                                                                                                                                gtattgggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAaagaa              >  1:484265/10‑251 (MQ=255)                                                                                                                                                                      ggcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTattgagc                                                         >  1:524696/4‑196 (MQ=255)                                                                                                                                                                       gcaTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGatg                                               <  1:529389/209‑2 (MQ=255)                                                                                                                                                                        caTTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACACCAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCatta      >  1:718472/2‑248 (MQ=255)                                                                                                                                                                            aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATaagt                                                                                                                    >  1:19652/1‑135 (MQ=255)                                                                                                                                                                            aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAAc                                                                                                         <  1:474995/148‑1 (MQ=255)                                                                                                                                                                            aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACa                                                                                                        <  1:462167/149‑1 (MQ=255)                                                                                                                                                                            aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTat                                                              <  1:899386/191‑2 (MQ=255)                                                                                                                                                                            aaCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTATTGAGCAATCTTGATGTTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCattagtaa  >  1:328079/1‑244 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                                          ATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGTACTAAGTAATCTTGATATTGAAAAGATTGATGAACTTCAAAGTGAAAGAAGCTCACTGGTAA  >  1/1482733‑1483154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑