Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,864	G→T	P317P (CCG→CCT)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,864	0	G	T	100.0%	33.6 / NA	15	P317P (CCG→CCT)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/9);  total (6/9)

ATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGT  >  1/1482733‑1483092                                                                                                                                    |                                                                                                                                                                                                                                     attcgcagtaCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGcc                                                                                                               <  1:883817/242‑1 (MQ=255)          aCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGaaaa                                                                                                      >  1:63248/1‑251 (MQ=255)                                     gtatGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                                                                                <  1:858971/211‑1 (MQ=255)                                       atGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                                                                               <  1:19406/196‑1 (MQ=255)                                               cTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCAtt                                                                 >  1:171135/1‑251 (MQ=255)                                                   taatcggttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                                                             <  1:506710/237‑1 (MQ=255)                                                          ttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATaagt                                                      <  1:231075/244‑3 (MQ=255)                                                             gaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                                                             >  1:847208/5‑241 (MQ=255)                                                                      aaGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACa                                          <  1:591750/251‑1 (MQ=255)                                                                         ttAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACAt                                         <  1:256312/249‑1 (MQ=255)                                                                                                  ccttAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                                                                               <  1:393820/136‑1 (MQ=255)                                                                                                           gTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATc                   <  1:402244/237‑1 (MQ=255)                                                                                                           gTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAgaagtga     >  1:44448/1‑251 (MQ=255)                                                                                                              cttcAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAGCTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACATGCTGACCAAACAACAAAAAATCTTACTTAgaagtgaagt  >  1:577410/1‑251 (MQ=255)                                                                                                              cttcAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                                                                                >  1:184587/1‑141 (MQ=255)                                                                                                                                    |                                                                                                                                                                                                                                     ATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACATGCTGACCAAACAACAAAAAATCTTACTTAGAAGTGAAGT  >  1/1482733‑1483092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑