Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,816	A→G	K301K (AAA→AAG)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,816	0	A	G	100.0%	31.7 / NA	13	K301K (AAA→AAG)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/9);  total (4/9)

GAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACAT  >  1/1482574‑1483053                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              gAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                                                                                       >  1:790560/1‑251 (MQ=255)  aaaaaTACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                                                                                       <  1:667290/250‑1 (MQ=255)       tACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAAc                                                                                                                                                                                                                                 <  1:818262/251‑1 (MQ=255)                                                                                                                                                                attcgcagtaCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGcc                                                                        <  1:883817/242‑1 (MQ=255)                                                                                                                                                                         aCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGaaaa                                                               >  1:63248/1‑251 (MQ=255)                                                                                                                                                                                                    gtatGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                                         <  1:858971/211‑1 (MQ=255)                                                                                                                                                                                                      atGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                                        <  1:19406/196‑1 (MQ=255)                                                                                                                                                                                                              cTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCAtt                          >  1:171135/1‑251 (MQ=255)                                                                                                                                                                                                                  taatcggttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                      <  1:506710/237‑1 (MQ=255)                                                                                                                                                                                                                         ttagaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATaagt               <  1:231075/244‑3 (MQ=255)                                                                                                                                                                                                                            gaagcAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAAc                      >  1:847208/5‑241 (MQ=255)                                                                                                                                                                                                                                     aaGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACa   <  1:591750/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCATTAAACAATAAGTAGTGGTTAAACAt  <  1:256312/249‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              GAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATTAAACAGTAAATAGTGGCTAAACAT  >  1/1482574‑1483053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑