Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,796	T→G	S295A (TCA→GCA)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,796	0	T	G	100.0%	19.8 / NA	9	S295A (TCA→GCA)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/6);  total (3/6)

CGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATT  >  1/1482569‑1483029                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          cgACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGctaagaat                                                                                                                                                                                                                    <  1:650576/251‑8 (MQ=255)      gAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                                                               >  1:790560/1‑251 (MQ=255)       aaaaaTACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                                                               <  1:667290/250‑1 (MQ=255)            tACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAAc                                                                                                                                                                                                         <  1:818262/251‑1 (MQ=255)                                                                                                                                                                     attcgcagtaCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGcc                                                <  1:883817/242‑1 (MQ=255)                                                                                                                                                                              aCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGaaaa                                       >  1:63248/1‑251 (MQ=255)                                                                                                                                                                                                         gtatGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGc                                                 <  1:858971/211‑1 (MQ=255)                                                                                                                                                                                                           atGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAatat                                                                <  1:19406/196‑1 (MQ=255)                                                                                                                                                                                                                   cTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGAAAAACAAAGTTTATTTTCCTCATTTTTTACAAAACTCAtt  >  1:171135/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          CGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAAACAGAGTTTATTTTCTTCATTTTTTTCAAAACTCATT  >  1/1482569‑1483029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑