Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,762	T→C	R283R (CGT→CGC)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,762	0	T	C	100.0%	17.2 / NA	8	R283R (CGT→CGC)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/6);  total (2/6)

ACATATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAA  >  1/1482530‑1482992                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       atatatTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGCTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTc                                                                                                                                                                                                                       <  1:860437/248‑1 (MQ=255)                                        cgACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGctaagaat                                                                                                                                                                               <  1:650576/251‑8 (MQ=255)                                             gAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                          >  1:790560/1‑251 (MQ=255)                                              aaaaaTACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat                                                                                                                                                                          <  1:667290/250‑1 (MQ=255)                                                   tACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAAc                                                                                                                                                                    <  1:818262/251‑1 (MQ=255)                                                       aTTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGtat                                                                                                                                                                                                                              <  1:417066/189‑3 (MQ=255)                                                                                                                                                                                                            attcgcagtaCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGcc           <  1:883817/242‑1 (MQ=255)                                                                                                                                                                                                                     aCTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAACCTTAATCGGTTCTTCAATAGATGCCATAATTCCTTTTGTTAAAAATACTAATACAAAAACTGTATTGGGCATTAACTTAGGTCGTGATGGGAAAAAGGAAATTAATTCACTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCAAAAGaaaa  >  1:63248/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       ACATATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAACTTTAATTGGTTCTTCAATAGATGCCGTAATCCCGTATATTAAAAACACTAATACAAAAACCATATTAGGTATTAACTTAGGTCGTGATGGTAAAAAGGAAATTAATTCATTAATGTTAAAGGTTATTGGTGCAATATCTCGCAGTAGTAAGCCGAAAGAAAA  >  1/1482530‑1482992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑