Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,693	G→T	S260S (TCG→TCT)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,693	0	G	T	100.0%	26.3 / NA	11	S260S (TCG→TCT)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/7);  total (4/7)

TTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATACTCCGAATTTAGACATATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAAC  >  1/1482469‑1482830                                                                                                                                                                                                                                 |                                                                                                                                          ttagatttaTTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATATATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGa                                                                                                                 <  1:32018/251‑1 (MQ=255)           tattTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATATATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAGCCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAg                                                                                                       <  1:26506/251‑1 (MQ=255)                        gCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATATATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAgcgtattcgcagta                                                                                          >  1:504066/1‑240 (MQ=255)                                       tATTGAATATGCTCCTAATTTAGATATATTTAACAGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCaa                                                                           >  1:206159/1‑251 (MQ=255)                                       caTTGAATATGCTCCTAATTTAGATATATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCaa                                                                           >  1:206128/2‑251 (MQ=255)                                                              atatatTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGCTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTc                                                     <  1:860437/248‑1 (MQ=255)                                                                                                     cgACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGctaagaat             <  1:650576/251‑8 (MQ=255)                                                                                                          gAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat        >  1:790560/1‑251 (MQ=255)                                                                                                           aaaaaTACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGatat        <  1:667290/250‑1 (MQ=255)                                                                                                                tACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGTATGTATTTCTGATAATCGGTTAGAAGCAGCTAAGTTAATGGCTAAGAATGATATCGAAAc  <  1:818262/251‑1 (MQ=255)                                                                                                                    aTTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCAATTCGCACTTTTGtat                                                            <  1:417066/189‑3 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                          TTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATACTCCGAATTTAGACATATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAATTCGTACTTTTGTCTGTATTTCTGATAATCGAATGGAATCAGCTAAGTTAATGGCCAAAAATGATATAGAAAC  >  1/1482469‑1482830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑