Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	246,800	T→G	V105V (GTA→GTC)	unknown ←	YcfF/hinT protein: a purine nucleoside phosphoramidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	246,800	0	T	G	100.0%	159.1 / NA	58	V105V (GTA→GTC)	unknown	YcfF/hinT protein: a purine nucleoside phosphoramidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/51);  total (7/51)

ACCAATACAGGCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCTACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGCGGTGACAAGCTC  >  1/246556‑247045                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aCCAATACAGGCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAgg                                                                                                                                                                                                                                                 >  1:694645/1‑251 (MQ=255)  ccAATACAGGCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGt                                                                                                                                                                                                                                                >  1:484629/1‑251 (MQ=255)           gCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACCCCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCatat                                                                                                                                                                                                                                          <  1:602584/248‑1 (MQ=255)           gCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         <  1:864745/249‑1 (MQ=255)           gCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         <  1:597761/249‑1 (MQ=255)           gCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         <  1:678373/249‑1 (MQ=255)           gCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCCTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         <  1:874372/249‑1 (MQ=255)            cTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAgg                                                                                                                                                                                                                                                 >  1:71461/1‑240 (MQ=255)            cTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTa                                                                                                                                                                                                                                       <  1:748748/250‑1 (MQ=255)            cTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTa                                                                                                                                                                                                                                       <  1:867015/250‑1 (MQ=255)            cTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTa                                                                                                                                                                                                                                       <  1:752388/250‑1 (MQ=255)             tGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAg                                                                                                                                                                                                                                     <  1:211284/251‑1 (MQ=255)             tGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAg                                                                                                                                                                                                                                     <  1:307274/251‑1 (MQ=255)               tAAGCCAAATCTACGCTGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGt                                                                                                                                                                                                                                    <  1:526866/250‑1 (MQ=255)                     aaaTCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAacac                                                                                                                                                                                                                             <  1:148876/251‑1 (MQ=255)                       aTCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACtt                                                                                                                                                                                                                           >  1:10045/1‑251 (MQ=255)                                gCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTccg                                                                                                                                                                                                                   >  1:434339/1‑250 (MQ=255)                                           tgttttTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTttgttg                                                                                                                                                                                                       <  1:726179/251‑1 (MQ=255)                                                            ttATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         <  1:440745/200‑1 (MQ=255)                                                            ttATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAAc                                                                                                                                                                                       <  1:110486/250‑1 (MQ=255)                                                                    gTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTg                                                                                                                                                                                                                                   <  1:574081/198‑1 (MQ=255)                                                                           ccGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGc                                                                                                                                                                       <  1:330179/251‑1 (MQ=255)                                                                           ccGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGc                                                                                                                                                                       <  1:838447/251‑1 (MQ=255)                                                                                 tCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGcc                                                                                                                                                                 <  1:293025/251‑1 (MQ=255)                                                                                     ctctTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCcttct                                                                                                                                                             <  1:752965/251‑1 (MQ=255)                                                                                       ctTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATg                                                                                                                                                                                                                                         >  1:485828/1‑173 (MQ=255)                                                                                           ccgccgCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAAGGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGgc                                                                                                                                                        <  1:193727/250‑1 (MQ=255)                                                                                                     ggCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACg                                                                                                                                                                                                                                                    <  1:499833/148‑1 (MQ=255)                                                                                                       cTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTggc                                                                                                                                            <  1:221345/250‑1 (MQ=255)                                                                                                                    aaTCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAAc                                                                                                                               <  1:198360/250‑1 (MQ=255)                                                                                                                           ttttGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGAcc                                                                                                                       <  1:291385/251‑1 (MQ=255)                                                                                                                              tGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTC‑‑AAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAagag                                                                                                                  <  1:94138/251‑1 (MQ=255)                                                                                                                                      cAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAg                                                                                                                                                                                                                                     <  1:316497/130‑1 (MQ=255)                                                                                                                                      cAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTg                                                                                                                                                           <  1:411190/204‑1 (MQ=255)                                                                                                                                      cAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACtt                                                                                                            <  1:556147/251‑1 (MQ=255)                                                                                                                                                                  gCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTaa                                                                                    <  1:508505/247‑1 (MQ=255)                                                                                                                                                                  gCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTaa                                                                                    <  1:162753/247‑1 (MQ=255)                                                                                                                                                                  gCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTaa                                                                                    <  1:167385/247‑1 (MQ=255)                                                                                                                                                                  gCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTaa                                                                                    <  1:550912/247‑1 (MQ=255)                                                                                                                                                                  gCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACgg                                                                                 <  1:816171/250‑1 (MQ=255)                                                                                                                                                                     gCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCt                                                                                                       >  1:75635/1‑225 (MQ=255)                                                                                                                                                                      cTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGc                                                                                                                                                                       <  1:664599/160‑1 (MQ=255)                                                                                                                                                                       tAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGa                                                                           <  1:171523/251‑1 (MQ=255)                                                                                                                                                                        aaaaGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGAt                                                                          <  1:220668/251‑1 (MQ=255)                                                                                                                                                                               cGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTa                                                                   <  1:303861/251‑1 (MQ=255)                                                                                                                                                                                                 aaaTTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTTCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAAtg                                                 <  1:429843/251‑1 (MQ=255)                                                                                                                                                                                                                  tatttatCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAAtt                                                                     <  1:823524/214‑1 (MQ=255)                                                                                                                                                                                                                    tttatCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGt                                                                                                                 <  1:631301/168‑1 (MQ=255)                                                                                                                                                                                                                       atCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATc                                                                                                        <  1:430087/174‑1 (MQ=255)                                                                                                                                                                                                                       atCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCGTTaa                                                                                    <  1:81493/194‑1 (MQ=255)                                                                                                                                                                                                                       atCCAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCaa                           <  1:200353/251‑1 (MQ=255)                                                                                                                                                                                                                          cAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAACCGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAatat                        <  1:704381/251‑1 (MQ=255)                                                                                                                                                                                                                          cAACATTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAatat                        <  1:120616/251‑1 (MQ=255)                                                                                                                                                                                                                              aTTTTGCCCAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTAATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAatat                        <  1:403028/247‑1 (MQ=255)                                                                                                                                                                                                                                    cccAACGGTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGc              <  1:24701/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggTTCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAAtt                                                                     <  1:533819/190‑1 (MQ=255)                                                                                                                                                                                                                                            ttCGCCGCCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGCGGTGACa       <  1:621355/250‑1 (MQ=255)                                                                                                                                                                                                                                              cgccgcCGACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGCGGTGACAAGc    <  1:843597/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccgccgACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGCGGTGACAAGCTc  <  1:624770/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ACCAATACAGGCTGTAAGCCAAATCTACGATGCGTCCGGTTTTGTTTTTCACCCAGGCGTTATCGCTGTTTACACCGGCTTCCACTCTTGCCGCCGCAGGGGCTTCAATGTTTAGAATCGGGTTTTGGGTGTTCAATAAATTCGGCGCTTTGGCGCTACAGGCCGCTAAAAGTGCGGTCAAAAAAAACAGTGAAATTGGTTTTTTCATTTATTTATCCAACATTTTGCCCAACGGTTCGCCGCCTACGAGGTGCATATGTAAGTGGAACACTTCCTGTCCGCCGTGTTTGTTGCAATTGACGATTAAACGATAGCCGTCTTGTGCGATGCCTTCTTGGGCAGCAAGTTTGGCGGCAACGGTGAACAGGCGACCAAGAGTTACTTCATCTTGTTCGGTCACATCATTAACGGTCGGGATCAATTTATTCGGAATGATTAAAATGTGGGTTTTTGCTTGTGGCGCAATATCGCGAAAGGCGGTGACAAGCTC  >  1/246556‑247045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑