Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,482,522	G→T	P203P (CCG→CCT)	unknown →	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,482,522	0	G	T	100.0%	84.1 / NA	33	P203P (CCG→CCT)	unknown	Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/21);  total (12/21)

AAGTCAGATGGTTGAGAAAATCATTTTAGGTGCTGATATCCCTCGCTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAAGGTGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTGTCAAGTAAAAAAATTCCAGTGCTATTAGCCCAAGGCCCTAATGGTTCTCAAGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATACTCCGAATTTAGACAT‑ATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAA  >  1/1482279‑1482757                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             aaGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAg                                                                                                                                                                                                                                       <  1:589932/251‑1 (MQ=255) aaGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAg                                                                                                                                                                                                                                       <  1:749870/251‑1 (MQ=255)                  aaaTCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTCAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAgc                                                                                                                                                                                                                     <  1:117216/251‑2 (MQ=255)                         tttAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTg                                                                                                                                                                                                              <  1:21137/251‑1 (MQ=255)                                   gATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGata                                                                                                                                                                                                                                    >  1:732294/1‑218 (MQ=255)                                                 aaGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGTCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGaaa                                                                                                                                                                                      <  1:538659/251‑1 (MQ=255)                                                      aTACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATAc                                                                                                                                                                                 <  1:407631/251‑1 (MQ=255)                                                                  ttGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCt                                                                                                                                                                                                          >  1:347677/1‑214 (MQ=255)                                                                       gTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAgcttatt                                                                                                                                                                                                                >  1:269380/1‑197 (MQ=255)                                                                       gTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGc                                                                                                                                                                                                           <  1:748852/208‑1 (MQ=255)                                                                              gTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAAc                                                                                                                                                           >  1:252111/1‑249 (MQ=255)                                                                                   aagggcggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACctatcta                                                                                                                                                    <  1:367582/245‑1 (MQ=255)                                                                                      ggcggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACctatctat                                                                                                                                                   <  1:7724/246‑1 (MQ=255)                                                                                        cggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:817751/249‑2 (MQ=255)                                                                                        cggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:711880/249‑2 (MQ=255)                                                                                        cggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:477338/249‑2 (MQ=255)                                                                                        cggtATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:556451/249‑2 (MQ=255)                                                                                                gcgcAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGTTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTGCGATCAACCTATCTATaacgtcaaaaa                                                                                                                                        <  1:158595/250‑10 (MQ=255)                                                                                                                    caTATTGCTAATGAGATTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTg                                                                                                                                                                                                              <  1:154381/160‑1 (MQ=255)                                                                                                                                  gaTTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTg                                                                                                                                                                                                              <  1:861778/146‑1 (MQ=255)                                                                                                                                  gaTTGTATCAAGTAAAAAAATTCCGGTGCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGAt                                                                                                                                                               >  1:637905/1‑193 (MQ=255)                                                                                                                                                             gCTATTAGCCCAAGGTCCTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTagaaagaaaaa                                                                          >  1:496521/1‑248 (MQ=255)                                                                                                                                                                           gtccTAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAAc                                                                                                                                                                                                                           >  1:148247/3‑92 (MQ=255)                                                                                                                                                                               tAATGGTTCCCAGGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGAt                                                                                                                                                               >  1:470018/1‑148 (MQ=255)                                                                                                                                                                                               ttagatttaTTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGa                                        <  1:32018/251‑1 (MQ=255)                                                                                                                                                                                                         tattTGATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAGCCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAg                              <  1:26506/251‑1 (MQ=255)                                                                                                                                                                                                              gATAAAAAGCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATATAATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:467729/133‑2 (MQ=255)                                                                                                                                                                                                                      gCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                <  1:634047/124‑2 (MQ=255)                                                                                                                                                                                                                      gCTATCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAgcgtattcgcagta                 >  1:504066/1‑240 (MQ=255)                                                                                                                                                                                                                         aTCAGGTAATGTTATTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTataataa                                                                                                    <  1:573624/165‑2 (MQ=255)                                                                                                                                                                                                                                     tATTGAATATGCTCCTAATTTAGATAT‑ATTTAACAGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCaa  >  1:206159/1‑251 (MQ=255)                                                                                                                                                                                                                                     caTTGAATATGCTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAACGTCAAAAAGGATAATTTCATTAGTTTTTTAGAAAACTATAATAATTTCGTATTAGTTGTAGAAAGAAAAATCGGTTCTCTACGTTTAGCTAAACAGTTTCTAGATGAATGTGAGCGTATTCGCAGTACTTCGAGAAAACCaa  >  1:206128/2‑251 (MQ=255)                                                                                                                                                                                                                                               gcTCCTAATTTAGATAT‑ATTTAACGGGAGCTTATTTGAGCTAACACCCGCAGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATaac                                                                                                                                                >  1:306341/2‑98 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             AAGTCAGATGGTTGAGAAAATCATTTTAGGTGCTGATATCCCTCGCTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAAGGTGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTGTCAAGTAAAAAAATTCCAGTGCTATTAGCCCAAGGCCCTAATGGTTCTCAAGATTTAGATTTATTATTTGATAAAAAGCTATCAGGTAATGTTATTGAATATACTCCGAATTTAGACAT‑ATTTAACGGGAGTCTATTTGAGCTAACACCTGCGGCGACTGAAAAATACAATTTTATTATCTACGATCAACCTATCTATAATGTTAAGAAGGATAATTTCATTGGTTTTTTAGAAAACTATAATAGTTTCGTATTAGTTGTAGAAAGAAGAATTGGTTCGCTACGTTTAGCTAAGCAGTTCCTAGATGAATGCGAGCGCATCCGTGGCACTTCGAGAAAACCAA  >  1/1482279‑1482757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑