Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,482,169 A→G I86V (ATA→GTA)  unknown → Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,482,1690AG100.0% 224.7 / NA 78I86V (ATA→GTA) unknownType II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/37);  total (41/37)

TCAAGAAACAATCACGGCAGACAGTGCTCGCACTATTACTATAGTGTCAAGCCGTGATGATATCCAAGGTGAAGTTGCACAAATTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTCACCTCATCTGATGAAATTTCATTTTCAGCAGAAGACACTGTAGGGGTTATTATTGATATCACACATGAGACTAACATAAAGACTATTGTCGAACGAGTTTTTAGTATAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCCATCTCTTTATCACAAAAACTACTAGATGAGGGCATCCTTTACTTTAATTCGCATACTCAGTTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGCTGATATCCCTCGCTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAAGGTGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTGT  >  1/1481928‑1482415
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      
tCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACcac                                                                                                                                                                                                                                               <  1:78445/251‑1 (MQ=255)
                        tgcacgtaCTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGgtgttgtgt                                                                                                                                                                                                                            <  1:780066/239‑1 (MQ=255)
                         gcacgtaCTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACaa                                                                                                                                                                                                                                             <  1:278232/222‑1 (MQ=255)
                              taCTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGAt                                                                                                                                                                                                                  >  1:560341/2‑250 (MQ=255)
                              taCTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATa                                                                                                                                                                                                                 >  1:791165/2‑251 (MQ=255)
                                cTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTAt                                                                                                                                                                                                                         >  1:728572/1‑241 (MQ=255)
                                cTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGAt                                                                                                                                                                                                                  <  1:820219/248‑1 (MQ=255)
                                      ctgtaGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTAc                                                                                                                                                                                                                                                  <  1:566742/207‑1 (MQ=255)
                                           gtatCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAg                                                                                                                                                                                                                >  1:303041/4‑239 (MQ=255)
                                                aaGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACaa                                                                                                                                                                                                                                             >  1:498813/1‑205 (MQ=255)
                                                         tgatATTCAAGGTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAac                                                                                                                                                                                      <  1:101743/251‑1 (MQ=255)
                                                                    gTGAAGTTGCACAAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATg                                                                                                                                                                             <  1:58988/249‑1 (MQ=255)
                                                                             cacaAACTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGCGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCctt                                                                                                                                                                  >  1:619879/1‑251 (MQ=255)
                                                                                 aactCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATg                                                                                                                                                                             <  1:594277/233‑1 (MQ=255)
                                                                                  actCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCctttactt                                                                                                                                                             <  1:42275/249‑1 (MQ=255)
                                                                                   ctCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACt                                                                                                                                                                                                           >  1:551800/2‑204 (MQ=255)
                                                                                     cTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGAt                                                                                                                                                                                                                  <  1:105799/195‑1 (MQ=255)
                                                                                     cTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTaa                                                                                                                                                          >  1:547299/1‑251 (MQ=255)
                                                                                           aCTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCa                                                                                                                                                    >  1:834043/1‑251 (MQ=255)
                                                                                                    cTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATAcccaatt                                                                                                                                           >  1:305685/1‑245 (MQ=255)
                                                                                                        aaaaTATTGAGATTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGt                                                                                                                                       <  1:498609/251‑1 (MQ=255)
                                                                                                                  gaTTGTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTg                                                                                                                             >  1:81503/1‑251 (MQ=255)
                                                                                                                      gTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATgg                                                                                                                                                                                                                                    >  1:406740/1‑144 (MQ=255)
                                                                                                                      gTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCttt                                                                                                                                                                                                 >  1:534043/1‑179 (MQ=255)
                                                                                                                      gTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCtt                                                                                                                                                                                                  >  1:133029/1‑178 (MQ=255)
                                                                                                                      gTTAAAAAGGATTTTTTTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTgaga                                                                                                                          <  1:331546/250‑1 (MQ=255)
                                                                                                                                   ttttTACCTCGTCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATcac                                                                                                                                                                                            >  1:711300/1‑171 (MQ=255)
                                                                                                                                            cgtCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatata                                                                                                   >  1:294837/3‑250 (MQ=255)
                                                                                                                                              tCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatatac                                                                                                  <  1:642527/250‑3 (MQ=255)
                                                                                                                                              tCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatatac                                                                                                  <  1:894702/250‑3 (MQ=255)
                                                                                                                                              tCTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatatac                                                                                                  <  1:223233/250‑3 (MQ=255)
                                                                                                                                               cTGATGAGATTTCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGAGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCt                                                                                                <  1:232166/251‑1 (MQ=255)
                                                                                                                                                         ttCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      <  1:497566/251‑1 (MQ=255)
                                                                                                                                                         ttCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      <  1:368818/251‑1 (MQ=255)
                                                                                                                                                         ttCATTTTCAGCAGAAGATACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      <  1:809055/251‑1 (MQ=255)
                                                                                                                                                                      gaagaTACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACaa                                                                                                                                                                                                                                             >  1:811510/1‑87 (MQ=255)
                                                                                                                                                                      gaagaTACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAAt                                                                         >  1:293829/1‑251 (MQ=255)
                                                                                                                                                                      gaagaTACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGACGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGAGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAAt                                                                         >  1:272846/1‑251 (MQ=255)
                                                                                                                                                                       aagaTACTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTaaaa                                                                          <  1:494435/249‑1 (MQ=255)
                                                                                                                                                                        agatacTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGAt                                                                                                                                                                              >  1:370620/5‑148 (MQ=255)
                                                                                                                                                                        agatacTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAAt                                                                         <  1:569257/245‑1 (MQ=255)
                                                                                                                                                                          atacTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAag                                                                                        >  1:484173/3‑232 (MQ=255)
                                                                                                                                                                            acTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatatac                                                                                                  >  1:654308/1‑218 (MQ=255)
                                                                                                                                                                             cTGTAGGGATTATTATTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCAt                                                                                                                   <  1:648091/202‑1 (MQ=255)
                                                                                                                                                                                  gggattattaTTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCctttactt                                                                                                                                                             >  1:310216/5‑155 (MQ=255)
                                                                                                                                                                                    gattattaTTGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGa                                                                                                                                                                               <  1:327238/133‑1 (MQ=255)
                                                                                                                                                                                        attattGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTATCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAAt                                                                         <  1:456535/233‑1 (MQ=255)
                                                                                                                                                                                        attattGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATgg                                                                                                                                                                                                                                    <  1:51721/78‑1 (MQ=255)
                                                                                                                                                                                           attGATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATcc                                                                                                                                                                    <  1:659086/139‑1 (MQ=255)
                                                                                                                                                                                              gATATCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      >  1:586589/1‑214 (MQ=255)
                                                                                                                                                                                               atatCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATAcccaatta                                                                                                                                          <  1:271883/161‑8 (MQ=255)
                                                                                                                                                                                               atatCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATAc                                                                                                                                                 <  1:878003/154‑1 (MQ=255)
                                                                                                                                                                                               atatCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      >  1:103839/1‑213 (MQ=255)
                                                                                                                                                                                                tatCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCAtttt                                                                                                                >  1:295976/1‑186 (MQ=255)
                                                                                                                                                                                                  tCACACATGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCAC‑AAAACTACTAGATGAGGGTATCctttactt                                                                                                                                                             <  1:773383/138‑1 (MQ=255)
                                                                                                                                                                                                       caTGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCt                                                                                                <  1:807484/195‑1 (MQ=255)
                                                                                                                                                                                                       caTGAAACTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGgcg                                         >  1:307409/1‑250 (MQ=255)
                                                                                                                                                                                                         tgaaaCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCaa                                      >  1:606354/5‑251 (MQ=255)
                                                                                                                                                                                                           aaaCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTAc                                                                                                                                                                                                                                                  >  1:853849/3‑45 (MQ=255)
                                                                                                                                                                                                           aaaCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      >  1:770961/3‑201 (MQ=255)
                                                                                                                                                                                                           aaaCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      <  1:778527/199‑1 (MQ=255)
                                                                                                                                                                                                           aaaCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGt                                    >  1:170454/3‑251 (MQ=255)
                                                                                                                                                                                                             aCTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGatatac                                                                                                  <  1:641805/187‑3 (MQ=255)
                                                                                                                                                                                                              cTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGgt                                                                                                                                                                                                                                   >  1:246077/1‑57 (MQ=255)
                                                                                                                                                                                                              cTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACt                                                                                                                                                                                                           <  1:892275/81‑1 (MQ=255)
                                                                                                                                                                                                              cTAATATAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTa                                 >  1:230379/1‑251 (MQ=255)
                                                                                                                                                                                                                aataTAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAAt                               >  1:634745/4‑251 (MQ=255)
                                                                                                                                                                                                                 ataTAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAAtt                              <  1:589530/249‑1 (MQ=255)
                                                                                                                                                                                                                  taTAAAGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTAt                                              >  1:582757/2‑234 (MQ=255)
                                                                                                                                                                                                                     aaaGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCctttac                                                                                                                                                               >  1:360554/1‑118 (MQ=255)
                                                                                                                                                                                                                     aaaGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATAc                                                                                  >  1:19207/1‑195 (MQ=255)
                                                                                                                                                                                                                     aaaGACTATTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGt                                                            >  1:552469/1‑217 (MQ=255)
                                                                                                                                                                                                                            aTTGTCGAACGAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACatat                   >  1:729575/1‑251 (MQ=255)
                                                                                                                                                                                                                                      gAGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAGTTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGt                                                            <  1:631815/200‑1 (MQ=255)
                                                                                                                                                                                                                                       aGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAAtt                                                                                                                                                        <  1:306363/107‑1 (MQ=255)
                                                                                                                                                                                                                                       aGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAagag                                                                                      <  1:824016/173‑1 (MQ=255)
                                                                                                                                                                                                                                       aGTTTTTAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTaagggcgg                                                 >  1:645203/1‑204 (MQ=255)
                                                                                                                                                                                                                                         tttttAGTGTAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTaaaa                                                                          >  1:334762/1‑183 (MQ=255)
                                                                                                                                                                                                                                             tagtgtagtaCCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCAATCTCTTTATCACAAAAACTACTAGATGAGGGTATCCTTTACTTTAATTCGCATACCCAATTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGTTGATATACCTCGTTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAGGGCGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATtgtat  <  1:870742/251‑3 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      
TCAAGAAACAATCACGGCAGACAGTGCTCGCACTATTACTATAGTGTCAAGCCGTGATGATATCCAAGGTGAAGTTGCACAAATTCTACGTACTCGCGGTCTTGAAAATATTGAGATTGTTAAAAAGGATTTTTTCACCTCATCTGATGAAATTTCATTTTCAGCAGAAGACACTGTAGGGGTTATTATTGATATCACACATGAGACTAACATAAAGACTATTGTCGAACGAGTTTTTAGTATAGTACCACAAAATGTATGGTGTTGTGTTATCGGTGATAGTGACTCCATCTCTTTATCACAAAAACTACTAGATGAGGGCATCCTTTACTTTAATTCGCATACTCAGTTAAGTCAGATGGTTGAGAAAATCATTTTAGGTGCTGATATCCCTCGCTTAAGAGATACTGTTAAAATTGCTGTATTAAGTTGTAAAGGTGGTATTGGCGCAAGTTTAATTAGTTCACATATTGCTAATGAGATTGTGT  >  1/1481928‑1482415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑