Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,795	A→G	K134R (AAA→AGA)	unknown →	Flp pilus assembly protein RcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,795	0	A	G	100.0%	152.7 / NA	55	K134R (AAA→AGA)	unknown	Flp pilus assembly protein RcpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/31);  total (24/31)

ATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCTCGCACTATTACTATAGTGTCAAGCCGTGATGATATCCAAGGTGAAGTTGCACAAATTCTACGTACT  >  1/1481567‑1482021                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   aTGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                                                                                                                                                              <  1:1760/251‑1 (MQ=255)          ataGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCCTGTGCATATCGTACCGGAGaa                                                                                                                                                                                                     >  1:475489/1‑251 (MQ=255)               aaTTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAagtagt                                                                                                                                                                                                >  1:696406/1‑251 (MQ=255)                         attctgtcaTTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                                                                                                                                                              <  1:259751/219‑1 (MQ=255)                                      tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATc                                                                                                                                                                                                                >  1:680285/1‑212 (MQ=255)                                      tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGActt                                                                                                                                                                         >  1:751871/1‑251 (MQ=255)                                          ataGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtg                                                                                                                                                                     <  1:385612/251‑1 (MQ=255)                                             ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGc                                                                                                                                                                                                                     <  1:27961/200‑1 (MQ=255)                                             ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                                                                                                                                                                   >  1:46376/1‑250 (MQ=255)                                             ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                                                                                                                                                                   >  1:403827/1‑250 (MQ=255)                                              gtagtaACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCa                                                                                                                                                                 >  1:507635/1‑251 (MQ=255)                                                                    cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt                                                                                                                                            >  1:573292/1‑250 (MQ=255)                                                                    cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt                                                                                                                                            >  1:30624/1‑250 (MQ=255)                                                                          caTTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTg                                                                                                                                      <  1:219089/249‑1 (MQ=255)                                                                            tttGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAg                                                                                                                                   <  1:41621/251‑1 (MQ=255)                                                                                  gTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTaa                                                                                                                             >  1:594764/1‑251 (MQ=255)                                                                                   tGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTa                                                                                                                                                       <  1:49029/224‑1 (MQ=255)                                                                                     aTGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          >  1:108104/1‑251 (MQ=255)                                                                                     aTGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          <  1:111727/251‑1 (MQ=255)                                                                                                  gaaagCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAAt                                                                                                                            <  1:207813/236‑1 (MQ=255)                                                                                                   aaagCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTAtttttt                                                                                                              <  1:392321/249‑1 (MQ=255)                                                                                                       ccGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAg                                                                                                                       <  1:679031/236‑1 (MQ=255)                                                                                                           aaTGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTAc                                                                                                    <  1:668755/251‑1 (MQ=255)                                                                                                                 aaTTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATc                                                                                              <  1:299625/251‑1 (MQ=255)                                                                                                                                gATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          <  1:405803/208‑1 (MQ=255)                                                                                                                                 aTTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACgg                                                                               <  1:349204/250‑1 (MQ=255)                                                                                                                                        aTGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGt                                                                       >  1:117945/1‑251 (MQ=255)                                                                                                                                                 ccTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGcacgtac                                                               <  1:482277/250‑7 (MQ=255)                                                                                                                                                 ccTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGcacgta                                                                >  1:810929/1‑244 (MQ=255)                                                                                                                                                    ctAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          <  1:352969/188‑1 (MQ=255)                                                                                                                                                      aaaTCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTAc                                                         <  1:862273/251‑1 (MQ=255)                                                                                                                                                      aaaTCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTAc                                                         <  1:244043/251‑1 (MQ=255)                                                                                                                                                        atccaTTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACtg                                                       <  1:377486/247‑2 (MQ=255)                                                                                                                                                         tccaTTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACtgt                                                      <  1:453893/248‑3 (MQ=255)                                                                                                                                                          ccaTTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACt                                                                                                   >  1:653752/3‑205 (MQ=255)                                                                                                                                                                aCTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAg                                                                                                                                                         <  1:230080/145‑1 (MQ=255)                                                                                                                                                                                       gCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATg                                                                                                           >  1:612077/1‑168 (MQ=255)                                                                                                                                                                                          aaaaTATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGt                     <  1:28804/251‑1 (MQ=255)                                                                                                                                                                                                tatCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          >  1:524249/1‑144 (MQ=255)                                                                                                                                                                                                  tCCAATCTATATTGAAGTAGCTCAAATTGCCCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACaaa             >  1:101749/1‑251 (MQ=255)                                                                                                                                                                                                     aaTCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTg                                                                                                                                      >  1:507321/1‑127 (MQ=255)                                                                                                                                                                                                     aaTCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACtgt                                                      <  1:484886/207‑3 (MQ=255)                                                                                                                                                                                                      aTCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          <  1:554554/138‑1 (MQ=255)                                                                                                                                                                                                      aTCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACaaact           >  1:659905/1‑247 (MQ=255)                                                                                                                                                                                                      aTCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACaaact           >  1:200446/1‑247 (MQ=255)                                                                                                                                                                                                          atatTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATc                                                                                                                                                    >  1:793134/1‑108 (MQ=255)                                                                                                                                                                                                          atatTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGtta                                                                                                        <  1:647247/152‑1 (MQ=255)                                                                                                                                                                                                          atatTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGt     >  1:170678/1‑251 (MQ=255)                                                                                                                                                                                                           tatTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTa    <  1:286019/251‑1 (MQ=255)                                                                                                                                                                                                            atTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTAc   <  1:690801/251‑1 (MQ=255)                                                                                                                                                                                                             ttGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAGTATCAAGCCGTGATGATATTCAAGGTGAAGTTGCACAAACTCTACGTACt  <  1:649084/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATc                                                                                                                                                    >  1:644313/1‑98 (MQ=255)                                                                                                                                                                                                                    aGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                                                                                                                          <  1:544732/124‑1 (MQ=255)                                                                                                                                                                                                                    aGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTAc                                                         <  1:233191/189‑1 (MQ=255)                                                                                                                                                                                                                    aGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCACGTACTATTACTGTAgtatc                                                >  1:246256/1‑195 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   ATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTACTAGATCAAGAAACAATCACGGCAGACAGTGCTCGCACTATTACTATAGTGTCAAGCCGTGATGATATCCAAGGTGAAGTTGCACAAATTCTACGTACT  >  1/1481567‑1482021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑