Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,677	A→G	K95E (AAA→GAA)	unknown →	Flp pilus assembly protein RcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,677	0	A	G	100.0%	146.4 / NA	51	K95E (AAA→GAA)	unknown	Flp pilus assembly protein RcpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

TGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTAC  >  1/1481469‑1481923                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       tGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                                                                                                              <  1:467484/251‑1 (MQ=255)             cGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTAc                                                                                                                                                                                                      <  1:90780/247‑1 (MQ=255)                       gTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAg                                                                                                                                                                                         >  1:240469/1‑250 (MQ=255)                                ccGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGc                                                                                                                                                                               <  1:625946/251‑1 (MQ=255)                                   gTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCaaa                                                                                                                                                                            >  1:107084/1‑251 (MQ=255)                                          aTAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                                                                                                                     >  1:745632/1‑251 (MQ=255)                                                      aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATGGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                                                         >  1:470825/1‑251 (MQ=255)                                                      aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                                                         <  1:331429/251‑1 (MQ=255)                                                      aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                                                         >  1:741021/1‑251 (MQ=255)                                                         gTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGaa                                                                                                                                                      >  1:530630/1‑251 (MQ=255)                                                              caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                                                                                                              <  1:868739/190‑1 (MQ=255)                                                              caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                                                                                                                     >  1:832398/1‑231 (MQ=255)                                                                gTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCg                                                                                                                                                                                                                                                 <  1:486569/153‑1 (MQ=255)                                                                           gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                                                        <  1:163066/199‑1 (MQ=255)                                                                           gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                                                        >  1:802737/1‑199 (MQ=255)                                                                                   ttACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGa                                                                                                                                                       <  1:332138/224‑1 (MQ=255)                                                                                             attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATcc                                                                                                                                                                   <  1:737580/200‑1 (MQ=255)                                                                                             attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                                                                                                                     >  1:88097/3‑216 (MQ=255)                                                                                             attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTgcgcagaga                                                                                                                                 <  1:295766/234‑6 (MQ=255)                                                                                              ttctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAAt                                                                                                                                           <  1:744994/224‑1 (MQ=255)                                                                                                   aTGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                                                            <  1:1760/251‑1 (MQ=255)                                                                                                       cAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                                                                                                                     <  1:266230/206‑1 (MQ=255)                                                                                                          ttATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAAtt                                                                                                                                                                                                                                                   >  1:682100/1‑109 (MQ=255)                                                                                                            ataGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCCTGTGCATATCGTACCGGAGaa                                                                                                   >  1:475489/1‑251 (MQ=255)                                                                                                                 aaTTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAagtagt                                                                                              >  1:696406/1‑251 (MQ=255)                                                                                                                           attctgtcaTTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                                                            <  1:259751/219‑1 (MQ=255)                                                                                                                                   aTTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTatat                                                                                                                                                          >  1:725687/1‑173 (MQ=255)                                                                                                                                        tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATc                                                                                                              >  1:680285/1‑212 (MQ=255)                                                                                                                                        tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGActt                                                                       >  1:751871/1‑251 (MQ=255)                                                                                                                                            ataGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtg                                                                   <  1:385612/251‑1 (MQ=255)                                                                                                                                              aGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACAt                                                                                                                                                                                    >  1:417818/1‑136 (MQ=255)                                                                                                                                               ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                                                        >  1:309632/1‑131 (MQ=255)                                                                                                                                               ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAAt                                                                                                                                           >  1:81499/1‑176 (MQ=255)                                                                                                                                               ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGc                                                                                                                   <  1:27961/200‑1 (MQ=255)                                                                                                                                               ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                                                                 >  1:403827/1‑250 (MQ=255)                                                                                                                                               ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                                                                 >  1:46376/1‑250 (MQ=255)                                                                                                                                                gtagtaACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCa                                                               >  1:507635/1‑251 (MQ=255)                                                                                                                                                                      cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt                                          >  1:573292/1‑250 (MQ=255)                                                                                                                                                                      cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt                                          >  1:30624/1‑250 (MQ=255)                                                                                                                                                                            caTTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTg                                    <  1:219089/249‑1 (MQ=255)                                                                                                                                                                              tttGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAg                                 <  1:41621/251‑1 (MQ=255)                                                                                                                                                                                  gCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                                                                                                                     >  1:262190/1‑131 (MQ=255)                                                                                                                                                                                    gTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTaa                           >  1:594764/1‑251 (MQ=255)                                                                                                                                                                                     tGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTa                                                     <  1:49029/224‑1 (MQ=255)                                                                                                                                                                                       aTGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGcac                                                                                                                                                                                      <  1:790642/93‑1 (MQ=255)                                                                                                                                                                                       aTGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                        >  1:108104/1‑251 (MQ=255)                                                                                                                                                                                       aTGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAg                        <  1:111727/251‑1 (MQ=255)                                                                                                                                                                                                    gaaagCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAAt                          <  1:207813/236‑1 (MQ=255)                                                                                                                                                                                                     aaagCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTAtttttt            <  1:392321/249‑1 (MQ=255)                                                                                                                                                                                                         ccGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAg                     <  1:679031/236‑1 (MQ=255)                                                                                                                                                                                                             aaTGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTAc  <  1:668755/251‑1 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       TGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTTCAATTGAAGTATTAATAGAAGGTATTTTTTATGTTATTAC  >  1/1481469‑1481923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑