Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,640	A→C	I82I (ATA→ATC)	unknown →	Flp pilus assembly protein RcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,640	0	A	C	100.0%	127.7 / NA	45	I82I (ATA→ATC)	unknown	Flp pilus assembly protein RcpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/17);  total (28/17)

ACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTT  >  1/1481413‑1481883                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aCGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCAttt                                                                                                                                                                                                                                                 >  1:676897/1‑232 (MQ=255)    gCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa                                                                                                                                                                                                                           >  1:497763/1‑251 (MQ=255)     ccTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa                                                                                                                                                                                                                           <  1:767128/250‑1 (MQ=255)        ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCg                                                                                                                                                                                                                                             >  1:24003/1‑229 (MQ=255)        ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGc                                                                                                                                                                                                                        >  1:578163/1‑250 (MQ=255)          attgcattgGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCg                                                                                                                                                                                                                      >  1:228237/1‑250 (MQ=255)                                                         tGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                                                                      <  1:467484/251‑1 (MQ=255)                                                                     cGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTAc                                                                                                                                                              <  1:90780/247‑1 (MQ=255)                                                                               gTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAg                                                                                                                                                 >  1:240469/1‑250 (MQ=255)                                                                                        ccGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGc                                                                                                                                       <  1:625946/251‑1 (MQ=255)                                                                                           gTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCaaa                                                                                                                                    >  1:107084/1‑251 (MQ=255)                                                                                                  aTAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                                                                             >  1:745632/1‑251 (MQ=255)                                                                                                              aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATGGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                 >  1:470825/1‑251 (MQ=255)                                                                                                              aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                 <  1:331429/251‑1 (MQ=255)                                                                                                              aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                                                                                 >  1:741021/1‑251 (MQ=255)                                                                                                                 gTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGaa                                                                                                              >  1:530630/1‑251 (MQ=255)                                                                                                                      caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                                                                      <  1:868739/190‑1 (MQ=255)                                                                                                                      caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                                                                             >  1:832398/1‑231 (MQ=255)                                                                                                                        gTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCg                                                                                                                                                                                                         <  1:486569/153‑1 (MQ=255)                                                                                                                                   gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                >  1:802737/1‑199 (MQ=255)                                                                                                                                   gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                <  1:163066/199‑1 (MQ=255)                                                                                                                                           ttACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGa                                                                                                               <  1:332138/224‑1 (MQ=255)                                                                                                                                                     attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATcc                                                                                                                           <  1:737580/200‑1 (MQ=255)                                                                                                                                                     attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                                                                             >  1:88097/3‑216 (MQ=255)                                                                                                                                                     attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTgcgcagaga                                                                                         <  1:295766/234‑6 (MQ=255)                                                                                                                                                      ttctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAAt                                                                                                   <  1:744994/224‑1 (MQ=255)                                                                                                                                                           aTGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                    <  1:1760/251‑1 (MQ=255)                                                                                                                                                               cAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                                                                             <  1:266230/206‑1 (MQ=255)                                                                                                                                                                  ttATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAAtt                                                                                                                                                                                                           >  1:682100/1‑109 (MQ=255)                                                                                                                                                                    ataGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCCTGTGCATATCGTACCGGAGaa                                                           >  1:475489/1‑251 (MQ=255)                                                                                                                                                                         aaTTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAagtagt                                                      >  1:696406/1‑251 (MQ=255)                                                                                                                                                                                   attctgtcaTTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                                                                    <  1:259751/219‑1 (MQ=255)                                                                                                                                                                                           aTTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTatat                                                                                                                  >  1:725687/1‑173 (MQ=255)                                                                                                                                                                                                tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATc                                                                      >  1:680285/1‑212 (MQ=255)                                                                                                                                                                                                tGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGActt                               >  1:751871/1‑251 (MQ=255)                                                                                                                                                                                                    ataGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtg                           <  1:385612/251‑1 (MQ=255)                                                                                                                                                                                                      aGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACAt                                                                                                                                            >  1:417818/1‑136 (MQ=255)                                                                                                                                                                                                       ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                                                                                >  1:309632/1‑131 (MQ=255)                                                                                                                                                                                                       ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAAt                                                                                                   >  1:81499/1‑176 (MQ=255)                                                                                                                                                                                                       ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGc                                                                           <  1:27961/200‑1 (MQ=255)                                                                                                                                                                                                       ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                         >  1:46376/1‑250 (MQ=255)                                                                                                                                                                                                       ggTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTtgtg                         >  1:403827/1‑250 (MQ=255)                                                                                                                                                                                                        gtagtaACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCa                       >  1:507635/1‑251 (MQ=255)                                                                                                                                                                                                                              cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt  >  1:573292/1‑250 (MQ=255)                                                                                                                                                                                                                              cAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGtt  >  1:30624/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGTTTTTTGGAATTCTAATGAGACTTCTTGTGCACTTAAAAGTAATCAGCGTGTT  >  1/1481413‑1481883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑