Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,589	T→C	F65F (TTT→TTC)	unknown →	Flp pilus assembly protein RcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,589	0	T	C	100.0%	103.0 / NA	37	F65F (TTT→TTC)	unknown	Flp pilus assembly protein RcpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

GTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGT  >  1/1481342‑1481831                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAAt                                                                                                                                                                                                                                                 >  1:801448/1‑251 (MQ=255)                           cttcttAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTaa                                                                                                                                                                                                                       <  1:508324/251‑1 (MQ=255)                                    gaaTAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCaaaa                                                                                                                                                                                                              >  1:504045/2‑251 (MQ=255)                                        aGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACaa                                                                                                                                                                                                          >  1:211/1‑251 (MQ=255)                                                                        aCGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCAttt                                                                                                                                                                                             >  1:676897/1‑232 (MQ=255)                                                                           gCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa                                                                                                                                                                       >  1:497763/1‑251 (MQ=255)                                                                            ccTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa                                                                                                                                                                       <  1:767128/250‑1 (MQ=255)                                                                               ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCg                                                                                                                                                                                         >  1:24003/1‑229 (MQ=255)                                                                               ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGc                                                                                                                                                                    >  1:578163/1‑250 (MQ=255)                                                                                 attgcattgGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCg                                                                                                                                                                  >  1:228237/1‑250 (MQ=255)                                                                                    gcattgGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTaa                                                                                                                                                                                                                       <  1:222257/194‑1 (MQ=255)                                                                                                                                tGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                  <  1:467484/251‑1 (MQ=255)                                                                                                                                            cGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTAc                                                                                                          <  1:90780/247‑1 (MQ=255)                                                                                                                                                      gTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGatat                                                                                                                                                                                                                                >  1:895490/1‑119 (MQ=255)                                                                                                                                                      gTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAg                                                                                             >  1:240469/1‑250 (MQ=255)                                                                                                                                                               ccGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGc                                                                                   <  1:625946/251‑1 (MQ=255)                                                                                                                                                                  gTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCaaa                                                                                >  1:107084/1‑251 (MQ=255)                                                                                                                                                                         aTAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                         >  1:745632/1‑251 (MQ=255)                                                                                                                                                                                     aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATGGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                             >  1:470825/1‑251 (MQ=255)                                                                                                                                                                                     aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                             <  1:331429/251‑1 (MQ=255)                                                                                                                                                                                     aTCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATAtt                                                             >  1:741021/1‑251 (MQ=255)                                                                                                                                                                                        gTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGaa                                                          >  1:530630/1‑251 (MQ=255)                                                                                                                                                                                             caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAAt                                                                                                                  <  1:868739/190‑1 (MQ=255)                                                                                                                                                                                             caGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAatatat                                                                         >  1:832398/1‑231 (MQ=255)                                                                                                                                                                                               gTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCg                                                                                                                                                     <  1:486569/153‑1 (MQ=255)                                                                                                                                                                                                          gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                            >  1:802737/1‑199 (MQ=255)                                                                                                                                                                                                          gTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGc                                                                                            <  1:163066/199‑1 (MQ=255)                                                                                                                                                                                                                  ttACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGa                                                           <  1:332138/224‑1 (MQ=255)                                                                                                                                                                                                                            attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATcc                                                                       <  1:737580/200‑1 (MQ=255)                                                                                                                                                                                                                            attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                         >  1:88097/3‑216 (MQ=255)                                                                                                                                                                                                                            attctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTgcgcagaga                                     <  1:295766/234‑6 (MQ=255)                                                                                                                                                                                                                             ttctAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAAt                                               <  1:744994/224‑1 (MQ=255)                                                                                                                                                                                                                                  aTGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGt                <  1:1760/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAg                                                         <  1:266230/206‑1 (MQ=255)                                                                                                                                                                                                                                         ttATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAAtt                                                                                                                                                       >  1:682100/1‑109 (MQ=255)                                                                                                                                                                                                                                           ataGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCCTGTGCATATCGTACCGGAGaa       >  1:475489/1‑251 (MQ=255)                                                                                                                                                                                                                                                aaTTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGGAAATTCGTACAAAATTGATTAAAAATGGTATCGCCTCTAAATCCATTTACTTAGAGAAAAAAGCACATAAAGCAAAAATATATCCAATCTATATTGAAGTAGCTCAAATTGCGCAGAGACCGGCGCCTTGTGCATATCGTACCGGAGAagtagt  >  1:696406/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCGCAATGAAAATTCGTACAAAATTGATTAAAAATGGTATTACCTCTAAATCTATTTACTTAGAGAAAAAAGCACATAAAGCAAAAACATATCCAATCTATATTGAAGTAGCTCAAATTGCGCCCAAACCGGCACCTTGTGCATATCGTACCGGAGAAGTAGT  >  1/1481342‑1481831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑