Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,468	G→A	S25N (AGT→AAT)	unknown →	Flp pilus assembly protein RcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,468	0	G	A	100.0%	137.8 / NA	53	S25N (AGT→AAT)	unknown	Flp pilus assembly protein RcpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/24);  total (29/24)

CAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCG  >  1/1481238‑1481671                                                                                                                                                                                                                                       |                                                                                                                                                                                                            cAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAGTGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGa                                                                                                                                                                                         >  1:26189/1‑251 (MQ=255)          tagtaAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGcac                                                                                                                                                                                <  1:315531/251‑1 (MQ=255)             taAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAAc                                                                                                                                                                             <  1:569516/251‑1 (MQ=255)                                           aCCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTacac                                                                                                                                               <  1:886097/251‑1 (MQ=255)                                                         aaaaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGt                                                                                                                                 <  1:480649/251‑1 (MQ=255)                                                           aaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGTCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTa                                                                                                                               >  1:676821/1‑251 (MQ=255)                                                           aaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCg                                                                                                                                  >  1:424317/1‑248 (MQ=255)                                                                gAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATg                                                                                                                             >  1:830495/1‑248 (MQ=255)                                                                 aaCAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAt                                                                                                                          <  1:685583/250‑1 (MQ=255)                                                                   cAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTAc                                                                                                                       <  1:610757/251‑1 (MQ=255)                                                                            gATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTc                                                                                                                                       <  1:229865/226‑1 (MQ=255)                                                                             aTTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTattc                                                                                                             <  1:143061/251‑4 (MQ=255)                                                                                cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGt                                                                                                                                            >  1:750070/1‑217 (MQ=255)                                                                                cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaat                                                                                                                                    >  1:500870/1‑222 (MQ=255)                                                                                cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGt                                                                                                                            >  1:758341/1‑233 (MQ=255)                                                                                  tcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAt                                                                                                                          <  1:386682/233‑1 (MQ=255)                                                                                  tcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATg                                                                                                        <  1:438033/251‑1 (MQ=255)                                                                                   ccATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCg                                                                                                                     <  1:761327/237‑1 (MQ=255)                                                                                             ccGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAtt                                                                                                                         <  1:407852/223‑1 (MQ=255)                                                                                               gTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaa                                                                                                                                     <  1:845507/209‑3 (MQ=255)                                                                                               gTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGt                                                                                                                            >  1:5876/1‑218 (MQ=255)                                                                                                     gAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCg                                                                                                                     >  1:359177/1‑219 (MQ=255)                                                                                                         gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTa                                                                                                                               >  1:730276/1‑205 (MQ=255)                                                                                                         gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATg                                                                                                        >  1:160/1‑228 (MQ=255)                                                                                                         gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGt                                                                                                       <  1:882225/229‑1 (MQ=255)                                                                                                         gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAAt                                                                                 >  1:801448/1‑251 (MQ=255)                                                                                                              ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAAt                                                                                                                                                                  >  1:735330/1‑165 (MQ=255)                                                                                                              ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAg                                                                                                                                         >  1:542475/1‑190 (MQ=255)                                                                                                              ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTAttct                                                                                                            <  1:855606/219‑1 (MQ=255)                                                                                                                      cATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaa                                                                                                                                     <  1:465160/186‑3 (MQ=255)                                                                                                                      cATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTAc                                                                                                                       >  1:382933/1‑200 (MQ=255)                                                                                                                           aCATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTAcc                                                                                                                      <  1:465830/196‑1 (MQ=255)                                                                                                                            cATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAtt                                                                                                                         <  1:600156/192‑1 (MQ=255)                                                                                                                                   cttcttAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTaa                                                       <  1:508324/251‑1 (MQ=255)                                                                                                                                     tcttAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAAt                                                                                                                                                                  <  1:503614/142‑1 (MQ=255)                                                                                                                                     tcttAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaat                                                                                                                                    >  1:868363/1‑169 (MQ=255)                                                                                                                                      cttAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTg                                                                                                                                                              >  1:353717/1‑145 (MQ=255)                                                                                                                                            gaaTAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCaaaa                                              >  1:504045/2‑251 (MQ=255)                                                                                                                                                aGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACaa                                          >  1:211/1‑251 (MQ=255)                                                                                                                                                    ggTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAg                                                                                                                                                                >  1:867137/1‑129 (MQ=255)                                                                                                                                                    ggTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTacac                                                                                                                                               >  1:309365/1‑146 (MQ=255)                                                                                                                                                                gagaAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGAt                                                                                                                  >  1:528979/1‑163 (MQ=255)                                                                                                                                                                  gaAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAg                                                                                              >  1:121544/1‑181 (MQ=255)                                                                                                                                                                                aCGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCAttt                             >  1:676897/1‑232 (MQ=255)                                                                                                                                                                                  ggCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaa                                                                                                                                     <  1:372559/126‑3 (MQ=255)                                                                                                                                                                                   gCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa       >  1:497763/1‑251 (MQ=255)                                                                                                                                                                                    ccTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCaaagaa       <  1:767128/250‑1 (MQ=255)                                                                                                                                                                                       ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAg                                                                                                                                                                >  1:759196/1‑94 (MQ=255)                                                                                                                                                                                       ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCg                         >  1:24003/1‑229 (MQ=255)                                                                                                                                                                                       ccATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGc    >  1:578163/1‑250 (MQ=255)                                                                                                                                                                                         attgcattgGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTAACAACCAAAAACAAATCAATATCATTTGGCGTGATGCTACGGCAAAGAAAGCCg  >  1:228237/1‑250 (MQ=255)                                                                                                                                                                                            gcattgGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAATTCTGTCATTCGTGATATAGGTAGTaa                                                       <  1:222257/194‑1 (MQ=255)                                                                                                                                                                                                 ggCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACaa                                                                                                                                                                     <  1:681778/79‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                            CAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAATTCCGTTATTCGTGATATAGGTAGTAACAACCAAAAGCAAATCAATATAATTTGGCGTGATGCTACGGCAAAGAAAGCCG  >  1/1481238‑1481671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑