Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,353	A→G	K446K (AAA→AAG)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,353	0	A	G	100.0%	185.4 / NA	65	K446K (AAA→AAG)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/34);  total (31/34)

GGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAAT  >  1/1481108‑1481592                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ggCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACAtt                                                                                                                                                                                                                                            >  1:348098/1‑251 (MQ=255)  gCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTa                                                                                                                                                                                                                                           >  1:418738/1‑251 (MQ=255)    ttATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAAc                                                                                                                                                                                                                                         <  1:19889/251‑1 (MQ=255)     tATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACa                                                                                                                                                                                                                                        <  1:713673/251‑1 (MQ=255)      aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       <  1:734551/251‑1 (MQ=255)      aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       >  1:343454/1‑251 (MQ=255)      aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       <  1:570967/251‑1 (MQ=255)         cAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       >  1:627371/1‑248 (MQ=255)            cTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                                                                                                                                                  <  1:156225/250‑1 (MQ=255)              cAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAActt                                                                                                                                                                                                                                >  1:283624/1‑250 (MQ=255)                                    cAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                                                                                                                                                         <  1:397528/251‑1 (MQ=255)                                    cAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                                                                                                                                                         <  1:633376/251‑1 (MQ=255)                                            ctctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGaa                                                                                                                                                                                                 >  1:584154/1‑251 (MQ=255)                                              ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       >  1:19574/1‑211 (MQ=255)                                              ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                                                                                                                                                  >  1:191150/1‑216 (MQ=255)                                              ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                                                                                                                                                  >  1:374242/1‑216 (MQ=255)                                                         cATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                                                                                                                                                         <  1:350451/230‑1 (MQ=255)                                                                        ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                                                                                                                                                      <  1:299605/250‑1 (MQ=255)                                                                        ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                                                                                                                                                      <  1:677226/250‑1 (MQ=255)                                                                        ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                                                                                                                                                      <  1:390986/250‑1 (MQ=255)                                                                         tctttcttCCGTAATG‑AACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGtt                                                                                                                                                                                                              >  1:893751/1‑208 (MQ=255)                                                                            ttcttcCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGc                                                                                                                                                                  >  1:524493/1‑250 (MQ=255)                                                                                      atGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACa                                                                                                                                                                                                                                        >  1:673363/2‑170 (MQ=255)                                                                                      atGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTatcg                                                                                                                                                       >  1:25320/2‑248 (MQ=255)                                                                                           acaacTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACgg                                                                                                                                                                                  <  1:477827/219‑1 (MQ=255)                                                                                                        aTAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACgg                                                                                                                                                                                  <  1:47135/206‑1 (MQ=255)                                                                                                        aTAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTg                                                                                                                                     <  1:277222/251‑1 (MQ=255)                                                                                                          aaaaaaGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCa                                                                                                                                   >  1:743436/1‑251 (MQ=255)                                                                                                                  gttagttaTCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTaa                                                                                                                                                                                                                           >  1:521202/1‑155 (MQ=255)                                                                                                                                  aCAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                                                                                                                                                       <  1:328686/127‑1 (MQ=255)                                                                                                                                  aCAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTatcg                                                                                                                                                       >  1:47207/1‑204 (MQ=255)                                                                                                                                   cAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAGTGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGa                                                                                                          >  1:26189/1‑251 (MQ=255)                                                                                                                                            tagtaAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGcac                                                                                                 <  1:315531/251‑1 (MQ=255)                                                                                                                                               taAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAAc                                                                                              <  1:569516/251‑1 (MQ=255)                                                                                                                                                              aaaaaGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAg                                                                                                                                                                                           <  1:160012/143‑1 (MQ=255)                                                                                                                                                                        tgtttACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATc                                                                                                                                                                                                                                      <  1:866502/88‑1 (MQ=255)                                                                                                                                                                             aCCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTacac                                                                <  1:886097/251‑1 (MQ=255)                                                                                                                                                                                           aaaaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGt                                                  <  1:480649/251‑1 (MQ=255)                                                                                                                                                                                             aaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGTCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTa                                                >  1:676821/1‑251 (MQ=255)                                                                                                                                                                                             aaCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCg                                                   >  1:424317/1‑248 (MQ=255)                                                                                                                                                                                                  gAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATg                                              >  1:830495/1‑248 (MQ=255)                                                                                                                                                                                                   aaCAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAt                                           <  1:685583/250‑1 (MQ=255)                                                                                                                                                                                                     cAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTAc                                        <  1:610757/251‑1 (MQ=255)                                                                                                                                                                                                       aTGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACAt                                                                                                                                                                                                                                             <  1:580306/52‑1 (MQ=255)                                                                                                                                                                                                         ggAAAGATTCTTCCATACGACTCCGTTAAAGATTGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                                                                                                                                                      >  1:403531/1‑121 (MQ=255)                                                                                                                                                                                                              gATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTc                                                        <  1:229865/226‑1 (MQ=255)                                                                                                                                                                                                               aTTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTattc                              <  1:143061/251‑4 (MQ=255)                                                                                                                                                                                                                  cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGt                                                             >  1:750070/1‑217 (MQ=255)                                                                                                                                                                                                                  cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaat                                                     >  1:500870/1‑222 (MQ=255)                                                                                                                                                                                                                  cttcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGt                                             >  1:758341/1‑233 (MQ=255)                                                                                                                                                                                                                    tcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAt                                           <  1:386682/233‑1 (MQ=255)                                                                                                                                                                                                                    tcCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATg                         <  1:438033/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTatcg                                                                                                                                                       <  1:5970/124‑4 (MQ=255)                                                                                                                                                                                                                     ccATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCg                                      <  1:761327/237‑1 (MQ=255)                                                                                                                                                                                                                               ccGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTAtt                                          <  1:407852/223‑1 (MQ=255)                                                                                                                                                                                                                                 gTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTcaa                                                      <  1:845507/209‑3 (MQ=255)                                                                                                                                                                                                                                 gTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGt                                             >  1:5876/1‑218 (MQ=255)                                                                                                                                                                                                                                       gAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCg                                      >  1:359177/1‑219 (MQ=255)                                                                                                                                                                                                                                           gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTa                                                >  1:730276/1‑205 (MQ=255)                                                                                                                                                                                                                                           gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATg                         >  1:160/1‑228 (MQ=255)                                                                                                                                                                                                                                           gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGt                        <  1:882225/229‑1 (MQ=255)                                                                                                                                                                                                                                           gTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTATTCTAATGTCAGTTATAGTAATTTATTCAAt  >  1:801448/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAAt                                                                                   >  1:735330/1‑165 (MQ=255)                                                                                                                                                                                                                                                ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAg                                                          >  1:542475/1‑190 (MQ=255)                                                                                                                                                                                                                                                ttaCAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCAATCAATGAATTTGTTGACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCAATCGTTATGTATTACCGGATTAttct                             <  1:855606/219‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCAATCAGTGAATTTGTTAACGATACGACTGTTGCACAACCGGTACAAAATAGTGAATTTAATCGTACACAGTTAGTCGATCGTTATGTATTACCGGATTACTCTAATGTCAGTTATAGTAATTTATTTAAT  >  1/1481108‑1481592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑