Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	240,724	A→G	H198R (CAT→CGT)	unknown →	Uracil‑DNA glycosylase, family 1 (EC 3.2.2.27)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	240,724	0	A	G	100.0%	184.1 / NA	66	H198R (CAT→CGT)	unknown	Uracil‑DNA glycosylase, family 1 (EC 3.2.2.27)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

TGCTGAATTCGGTGTTAACCGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTATCCGCCCATCGCGGTTTTTTCGGTTGCCATCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACACCGGCGGAT  >  1/240484‑240959                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            tGCTGAATTCGGTGTTAACCGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTcc                                                                                                                                                                                                                                   <  1:322556/251‑1 (MQ=255) tGCTGAATTCGGTGTTAACCGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTcc                                                                                                                                                                                                                                   <  1:543661/251‑1 (MQ=255)              gtTAACCGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCtt                                                                                                                                                                                                                      >  1:862518/1‑251 (MQ=255)                    cGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTa                                                                                                                                                                                                                 >  1:696857/1‑250 (MQ=255)                     gTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTaca                                                                                                                                                                                                               >  1:604428/1‑251 (MQ=255)                        gAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGcc                                                                                                                                                                                                                             >  1:681664/1‑234 (MQ=255)                        gAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTacaac                                                                                                                                                                                                             <  1:49954/250‑1 (MQ=255)                              ggTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCa                                                                                                                                                                                                      >  1:772565/1‑251 (MQ=255)                               gTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTtc                                                                                                                                                                                                                                      >  1:29410/1‑218 (MQ=255)                                 cTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   <  1:879538/251‑1 (MQ=255)                                 cTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   <  1:579857/251‑1 (MQ=255)                                       cATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAGCCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:118409/250‑1 (MQ=255)                                       cATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:400765/250‑1 (MQ=255)                                       cATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:320619/250‑1 (MQ=255)                                       cATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:463312/250‑1 (MQ=255)                                       cATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:676291/250‑1 (MQ=255)                                         ttCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTacaac                                                                                                                                                                                                             <  1:598762/233‑1 (MQ=255)                                         ttCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   <  1:535199/243‑1 (MQ=255)                                           ctcATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              >  1:76239/1‑246 (MQ=255)                                                  cAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATTTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   <  1:429930/234‑1 (MQ=255)                                                   aaTTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTa                                                                                                                                                                                                 <  1:535735/235‑1 (MQ=255)                                                   aaTTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              >  1:198842/1‑238 (MQ=255)                                                                    tttCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGc                                                                                                                                                                <  1:611529/251‑1 (MQ=255)                                                                         ccGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGcc                                                                                                                                                                                                                             >  1:430831/1‑185 (MQ=255)                                                                         ccGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTtta                                                                                                                                                           >  1:195466/1‑251 (MQ=255)                                                                         ccGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTtta                                                                                                                                                           >  1:540235/1‑251 (MQ=255)                                                                           gATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTAc                                                                                                                                                                                                <  1:372506/212‑1 (MQ=255)                                                                                 gTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTAc                                                                                                                                                                                                <  1:37146/206‑1 (MQ=255)                                                                                   gATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTac                                                                                                                                                                                                                >  1:304811/1‑188 (MQ=255)                                                                                   gATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGaa                                                                                                                                                 >  1:310820/1‑251 (MQ=255)                                                                                         aaCCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTa                                                                                                                                           <  1:162754/251‑1 (MQ=255)                                                                                                      caACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGattat                                                                                                                              >  1:89204/1‑251 (MQ=255)                                                                                                         cGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGc                                                                                                                                                                                              <  1:165220/184‑1 (MQ=255)                                                                                                         cGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTATCTGGCAATTAGCTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTtc                                                                                                                           <  1:622994/251‑1 (MQ=255)                                                                                                                     gTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   >  1:732592/1‑167 (MQ=255)                                                                                                                              ttGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTAttt                                                                                                                            <  1:309714/229‑1 (MQ=255)                                                                                                                              ttGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCCAAAACCTTAGAttt                                                                                                      >  1:643354/1‑251 (MQ=255)                                                                                                                                        gTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTa                                                                                                                                                                                                 >  1:383849/1‑150 (MQ=255)                                                                                                                                        gTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAAc                                                                                                                                                                           >  1:632623/1‑172 (MQ=255)                                                                                                                                        gTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCa                                                                                            >  1:406438/1‑251 (MQ=255)                                                                                                                                         tCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAg                                                                                           <  1:387781/251‑1 (MQ=255)                                                                                                                                                  gAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCatat                                                                                                                                                                                                   <  1:159131/138‑1 (MQ=255)                                                                                                                                                   aaaaaaGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGaaaa                                                                                 <  1:360526/251‑1 (MQ=255)                                                                                                                                                              gTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGc                                                                                                                                                                                                                        <  1:759798/105‑1 (MQ=255)                                                                                                                                                              gTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTTCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAAt                                                                      >  1:852910/1‑251 (MQ=255)                                                                                                                                                                            aaaaaCATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACttt                                                        <  1:250251/251‑1 (MQ=255)                                                                                                                                                                             aaaaCATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGc                                                                                                                                                                >  1:534342/1‑146 (MQ=255)                                                                                                                                                                               aaCATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTg                                                     >  1:493058/1‑251 (MQ=255)                                                                                                                                                                                       gTGCTGACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAg                                             >  1:688002/1‑251 (MQ=255)                                                                                                                                                                                            gACATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCcaa                                         >  1:90623/1‑250 (MQ=255)                                                                                                                                                                                              cATCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCcaacaa                                      <  1:877088/251‑1 (MQ=255)                                                                                                                                                                                               aTCCGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAAt                                     <  1:892889/251‑1 (MQ=255)                                                                                                                                                                                                 ccGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTa                                   >  1:637730/1‑251 (MQ=255)                                                                                                                                                                                                 ccGTGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTa                                   >  1:535633/1‑251 (MQ=255)                                                                                                                                                                                                    tGCATCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATc                                <  1:875222/251‑1 (MQ=255)                                                                                                                                                                                                        tCCTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACa                            >  1:766995/1‑251 (MQ=255)                                                                                                                                                                                                         ccTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACAt                           <  1:500470/251‑1 (MQ=255)                                                                                                                                                                                                         ccTTCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACAt                           >  1:788724/1‑251 (MQ=255)                                                                                                                                                                                                            tCGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTAttt                                                                                                                            <  1:632940/151‑1 (MQ=255)                                                                                                                                                                                                             cGCCGTTGTCCGCCCATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTgc                       <  1:130504/251‑1 (MQ=255)                                                                                                                                                                                                                          ccATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGacac          <  1:711196/251‑1 (MQ=255)                                                                                                                                                                                                                           cATCGCGGTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACAcc         <  1:509907/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAAt                                     >  1:421258/1‑216 (MQ=255)                                                                                                                                                                                                                                  gTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACACCGGCGGat  >  1:311240/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACACCGGCGGat  >  1:214442/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTTTTTCGGTTGCCGTCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACACCGGCGGat  >  1:103296/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            TGCTGAATTCGGTGTTAACCGTGGAACAAGGTCTGGCGCATTCTCATGCCAATTGGGGCTGGGAAATTTTCACCGATAAAGTGATTGAAACCTTGAATTCACAACGGGAAAATCTGGTCTTTTTATTGTGGGGCAGTCCGGCGCAGAAAAAAGGGCGGTTTATTGACAGACAAAAACATTGCGTGCTGACATCCGTGCATCCTTCGCCGTTATCCGCCCATCGCGGTTTTTTCGGTTGCCATCATTTCTCCCAAGCCAATGCTTATTTACAACAACACCATATTACGCCGATTAACTGGCAATTAACTTCAATTTAGCTTTTATTAATAGGAATTTTTATGCTCGCGATTATTTCTCCGGCAAAAACCTTAGATTTTCAGTCCGCAGTGCCGGAAAAACTCCCGAAATTTCAACCGCACTTTTTGGCGCAAAGCCAACAATTAATCGACATTTGCCGCCGCCTGACACCGGCGGAT  >  1/240484‑240959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑