Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,233	G→A	V406V (GTG→GTA)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,233	0	G	A	100.0%	271.8 / NA	99	V406V (GTG→GTA)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/60);  total (39/60)

AACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAATCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCA  >  1/1480987‑1481463                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       atcAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCaa                                                                                                                                                                                                                                    >  1:352253/3‑251 (MQ=255)          caccatagctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           >  1:38271/11‑251 (MQ=255)          caccatagctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           <  1:852076/241‑1 (MQ=255)          caccatagctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           <  1:776905/241‑1 (MQ=255)            ccatagctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAAt                                                                                                                                                                                                                            >  1:170905/9‑248 (MQ=255)            ccatagctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATgta                                                                                                                                                                                                                         >  1:102933/9‑251 (MQ=255)                  ctggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAA‑TTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAAc                                                                                                                                                                                                                  <  1:573617/249‑1 (MQ=255)                   tggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAAc                                                                                                                                                                                                                  <  1:185719/250‑1 (MQ=255)                   tggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAAc                                                                                                                                                                                                                  <  1:216929/250‑1 (MQ=255)                    ggTAACTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAAc                                                                                                                                                                                                                  <  1:409790/250‑1 (MQ=255)                       aaCTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAg                                                                                                                                                                                                                                 >  1:516222/1‑232 (MQ=255)                       aaCTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGt                                                                                                                                                                                                              >  1:10381/1‑251 (MQ=255)                         cTATAACTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTaa                                                                                                                                                                                                            >  1:345520/1‑251 (MQ=255)                               cTTTGAGGGAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGaaa                                                                                                                                                                                                      >  1:706333/1‑251 (MQ=255)                                  tgagggagtCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAg                                                                                                                                                                                                   <  1:85344/243‑1 (MQ=255)                                         gtCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttgt                                                                                                                                                                                            >  1:460604/2‑249 (MQ=255)                                         gtCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttgt                                                                                                                                                                                            >  1:193632/2‑249 (MQ=255)                                           cGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAAc                                                                                                                                                                                                                                   <  1:71690/210‑1 (MQ=255)                                              gAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTAc                                                                                                                                                                                        >  1:402589/1‑250 (MQ=255)                                               aaTATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTAccc                                                                                                                                                                                      <  1:730686/251‑1 (MQ=255)                                                          cTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTAc                                                                                                                                                                                        <  1:87451/238‑1 (MQ=255)                                                          cTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGaaa                                                                                                                                                                           <  1:40447/251‑1 (MQ=255)                                                               aTACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAAcc                                                                                                                                                                      <  1:73225/251‑1 (MQ=255)                                                                  cccGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAAt                                                                                                                                                                                                                            <  1:664807/194‑1 (MQ=255)                                                                   ccGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAg                                                                                                                                                                                                                                 >  1:83664/1‑188 (MQ=255)                                                                    cGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGa                                                                                                                                                                                                  >  1:829034/1‑218 (MQ=255)                                                                            aaagtCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGaaa                                                                                                                                                         <  1:578680/247‑1 (MQ=255)                                                                               gtCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           <  1:329633/181‑1 (MQ=255)                                                                               gtCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGAt                                                                                                                                                      >  1:698302/2‑251 (MQ=255)                                                                                 cTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGAttc                                                                                                                                                    <  1:266667/251‑1 (MQ=255)                                                                                    tATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttgt                                                                                                                                                                                            >  1:637077/1‑206 (MQ=255)                                                                                    tATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGAttcttc                                                                                                                                                 <  1:523771/251‑1 (MQ=255)                                                                                     aTTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTcc                                                                                                                                                <  1:118996/251‑1 (MQ=255)                                                                                       ttGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCAt                                                                                                                                              <  1:367296/251‑1 (MQ=255)                                                                                          aagTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATAc                                                                                                                                            <  1:56696/248‑1 (MQ=255)                                                                                          aagTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATAc                                                                                                                                            <  1:726876/248‑1 (MQ=255)                                                                                          aagTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATAc                                                                                                                                            <  1:822528/248‑1 (MQ=255)                                                                                             tAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGAc                                                                                                                                         <  1:61851/250‑1 (MQ=255)                                                                                             tAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGAc                                                                                                                                         <  1:872205/250‑1 (MQ=255)                                                                                              aGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGt                                                                                                                                                                                               >  1:479905/1‑195 (MQ=255)                                                                                              aGCTAACGGAGAAAGTTTTATTATCGGGGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttg                                                                                                                                                                                             <  1:456642/197‑2 (MQ=255)                                                                                                cTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTc                                                                                                                                       <  1:301665/249‑1 (MQ=255)                                                                                                cTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTc                                                                                                                                       <  1:498393/249‑1 (MQ=255)                                                                                                       agaAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAg                                                                                                                               <  1:621188/250‑1 (MQ=255)                                                                                                       agaAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAg                                                                                                                               <  1:575743/250‑1 (MQ=255)                                                                                                       agaAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAg                                                                                                                               <  1:56193/250‑1 (MQ=255)                                                                                                         aaaGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGaa                                                                                                                             <  1:443364/250‑1 (MQ=255)                                                                                                         aaaGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGaa                                                                                                                             <  1:629169/250‑1 (MQ=255)                                                                                                            gTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAAcc                                                                                                                                                                                                                 <  1:868296/163‑1 (MQ=255)                                                                                                             ttttATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGt                                                                                                                          <  1:283383/249‑1 (MQ=255)                                                                                                                tattatCGGTAGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATATTGTTTACCCTGATTTTGAAAAATCCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTttatt                                                                                                                     <  1:890265/251‑1 (MQ=255)                                                                                                                  ttatCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGCTGTTTACCCTGATTTTGAAAAAACTGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTAc                                                                                                                   <  1:148992/251‑1 (MQ=255)                                                                                                                  ttatCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGACACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTAc                                                                                                                   <  1:612029/251‑1 (MQ=255)                                                                                                                        gTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACaagac                                                                                                              <  1:547811/250‑4 (MQ=255)                                                                                                                          ggCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           <  1:76018/139‑1 (MQ=255)                                                                                                                          ggCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGaa                                                                                                                             <  1:281560/233‑1 (MQ=255)                                                                                                                          ggCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACAtt                                                                                                           >  1:348098/1‑251 (MQ=255)                                                                                                                           gCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTa                                                                                                          >  1:418738/1‑251 (MQ=255)                                                                                                                             ttATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAAc                                                                                                        <  1:19889/251‑1 (MQ=255)                                                                                                                              tATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACa                                                                                                       <  1:713673/251‑1 (MQ=255)                                                                                                                               aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                      <  1:570967/251‑1 (MQ=255)                                                                                                                               aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                      >  1:343454/1‑251 (MQ=255)                                                                                                                               aTTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                      <  1:734551/251‑1 (MQ=255)                                                                                                                                  cAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                      >  1:627371/1‑248 (MQ=255)                                                                                                                                     cTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                 <  1:156225/250‑1 (MQ=255)                                                                                                                                       cAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAActt                                                                                               >  1:283624/1‑250 (MQ=255)                                                                                                                                            gACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATgg                                                                                                                                                            <  1:92958/184‑1 (MQ=255)                                                                                                                                             aCCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           >  1:868615/1‑120 (MQ=255)                                                                                                                                             aCCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttg                                                                                                                                                                                             <  1:353824/150‑2 (MQ=255)                                                                                                                                             aCCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGa                                                                                                                                                                                   <  1:450934/160‑1 (MQ=255)                                                                                                                                              ccTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACaagac                                                                                                              >  1:402423/1‑225 (MQ=255)                                                                                                                                                ttGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGAttcttc                                                                                                                                                 >  1:692825/1‑191 (MQ=255)                                                                                                                                                             cAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                        <  1:633376/251‑1 (MQ=255)                                                                                                                                                             cAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                        <  1:397528/251‑1 (MQ=255)                                                                                                                                                                     ctctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGaa                                                                >  1:584154/1‑251 (MQ=255)                                                                                                                                                                       ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGaa                                                                                                                             <  1:254106/188‑1 (MQ=255)                                                                                                                                                                       ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACAt                                                                                                      >  1:19574/1‑211 (MQ=255)                                                                                                                                                                       ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                 >  1:374242/1‑216 (MQ=255)                                                                                                                                                                       ctTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAAc                                                                                                 >  1:191150/1‑216 (MQ=255)                                                                                                                                                                                  cATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATgt                                                                                                                                                                                                                          <  1:274517/84‑1 (MQ=255)                                                                                                                                                                                  cATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTc                                                                                                                                       >  1:432591/1‑167 (MQ=255)                                                                                                                                                                                  cATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATAc                                                                        <  1:350451/230‑1 (MQ=255)                                                                                                                                                                                         aTTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATg                                                                                                                                                                                                                           >  1:424564/1‑76 (MQ=255)                                                                                                                                                                                                 ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                     <  1:299605/250‑1 (MQ=255)                                                                                                                                                                                                 ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                     <  1:677226/250‑1 (MQ=255)                                                                                                                                                                                                 ttctttctTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCcattgcat                                     <  1:390986/250‑1 (MQ=255)                                                                                                                                                                                                  tctttcttCCGTAATG‑AACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGtt                                                                             >  1:893751/1‑208 (MQ=255)                                                                                                                                                                                                     ttcttcCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGc                                 >  1:524493/1‑250 (MQ=255)                                                                                                                                                                                                               atGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACa                                                                                                       >  1:673363/2‑170 (MQ=255)                                                                                                                                                                                                               atGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTatcg                      >  1:25320/2‑248 (MQ=255)                                                                                                                                                                                                                  caacaaCTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGttgtt                                                                                                                                                                                           >  1:560922/1‑80 (MQ=255)                                                                                                                                                                                                                    acaacTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACgg                                                 <  1:477827/219‑1 (MQ=255)                                                                                                                                                                                                                     caacTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTa                                                                                                                                  >  1:460959/1‑137 (MQ=255)                                                                                                                                                                                                                                 aTAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAg                                                                                                                               <  1:475864/128‑1 (MQ=255)                                                                                                                                                                                                                                 aTAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACgg                                                 <  1:47135/206‑1 (MQ=255)                                                                                                                                                                                                                                 aTAAAAAAGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTg    <  1:277222/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaaaaGAGTTAGTTATCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTAAAGAATAGTGGGTTCATACAATGAGAAAATTAGTTATTACGGCCTCCATTGCATTGGCCTCAATTATCGTTCCGGCAAAAACATTTGCa  >  1:743436/1‑251 (MQ=255)                                                                                                                                                                                                                                           gttagttaTCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAGACATTAACATCTAACTTCTTaa                                                                                          >  1:521202/1‑155 (MQ=255)                                                                                                                                                                                                                                              agttaTCGTAGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTGTTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACaagac                                                                                                              >  1:759512/1‑129 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       AACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAATCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAACAGTAAATGTAGTAAAACCGGTAAATGAAAAAGATGTTATTTACCCTGATTTTGAAAAAACCGGAACAATGGAAAGATTCTTCCATACGACTCCGTTAAAGAATGTTTATTACAAAACATTAACATCTAACTTCTTAAAAAATAGTGGGTTCATACAATGAGAAAATTAGTTATCACGGCCTCCATTGCATTGGCCTCAATTACCGCCCCGGTAACAACATTTGCA  >  1/1480987‑1481463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑