Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,005	A→T	A330A (GCA→GCT)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,005	0	A	T	100.0%	182.0 / NA	68	A330A (GCA→GCT)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (38/30);  total (38/30)

ATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAA  >  1/1480766‑1481237                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          attaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATaa                                                                                                                                                                                                                                >  1:2316/1‑251 (MQ=255)                 aCCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCggg                                                                                                                                                                                                               >  1:522610/1‑251 (MQ=255)                    aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat                                                                                                                                                                                                            <  1:203713/251‑1 (MQ=255)                    aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat                                                                                                                                                                                                            <  1:33771/251‑1 (MQ=255)                       gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATAtt                                                                                                                                                                                                         >  1:183680/1‑251 (MQ=255)                         aaTGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc                                                                                                                                                                                                        >  1:54873/1‑250 (MQ=255)                             gTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc                                                                                                                                                                                                        >  1:610723/1‑246 (MQ=255)                                  gTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCttt                                                                                                                                                                                                <  1:858954/249‑1 (MQ=255)                                     cTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAc                                                                                                                                                                                           <  1:132618/251‑1 (MQ=255)                                              ccgaacATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGc                                                                                                                                                                                  <  1:503844/251‑1 (MQ=255)                                                     tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAACATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                                                                                                                             >  1:619916/1‑247 (MQ=255)                                                     tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAAc                                                                                                                                                                                                                               >  1:591550/1‑200 (MQ=255)                                                     tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                                                                                                                             >  1:42944/1‑247 (MQ=255)                                                     tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGA‑GGAAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                                                                                                                             >  1:699856/1‑247 (MQ=255)                                                               atcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAcc                                                                                                                                                                                          <  1:385210/222‑1 (MQ=255)                                                                tcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGaagt                                                                                                                                                                <  1:458771/248‑4 (MQ=255)                                                                 caggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTa                                                                                                                                                               >  1:299047/3‑251 (MQ=255)                                                                                  atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt                                                                                                                                              >  1:537119/1‑251 (MQ=255)                                                                                  atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt                                                                                                                                              >  1:672845/1‑251 (MQ=255)                                                                                     ttAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTttat                                                                                                                                           <  1:572700/251‑1 (MQ=255)                                                                                                   aaTCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGC‑AAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCt                                                                                                                                                                           <  1:213319/204‑1 (MQ=255)                                                                                                    aTCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATAT‑TAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                                                                                                                           <  1:610478/251‑1 (MQ=255)                                                                                                     tCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                                                                                                                           <  1:298449/251‑1 (MQ=255)                                                                                                        cATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAg                                                                                                                         >  1:686939/1‑250 (MQ=255)                                                                                                                    gagATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTAtt                                                                                                                                                                      >  1:302300/3‑193 (MQ=255)                                                                                                                     agATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTg                                                                                                           >  1:624027/2‑251 (MQ=255)                                                                                                                     agATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTg                                                                                                           >  1:260867/2‑251 (MQ=255)                                                                                                                              tGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                                                                                                                           >  1:373521/1‑226 (MQ=255)                                                                                                                                 ttcgccAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAAc                                                                                                <  1:619642/246‑1 (MQ=255)                                                                                                                                   cgccAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACgg                                                                                                                                                       <  1:476283/191‑1 (MQ=255)                                                                                                                                         caaTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTGTTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTc                                                                                         >  1:426553/1‑249 (MQ=255)                                                                                                                                         caaTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAAc                                                                                                <  1:306676/242‑1 (MQ=255)                                                                                                                                         caaTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAAc                                                                                                >  1:674654/1‑242 (MQ=255)                                                                                                                                         caaTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTc                                                                                         >  1:24560/1‑249 (MQ=255)                                                                                                                                           aTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTc                                                                                         >  1:836875/1‑247 (MQ=255)                                                                                                                                           aTTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCATAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTttatta                                                                                                                                         <  1:161047/199‑1 (MQ=255)                                                                                                                                              atatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAGTCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCtttt                                                                                  >  1:612332/4‑251 (MQ=255)                                                                                                                                              atatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat                                                                                                                                                                                                            <  1:599827/126‑1 (MQ=255)                                                                                                                                              atatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACa                                                                                               <  1:100170/235‑1 (MQ=255)                                                                                                                                               tatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAg                                                                                                                                                                                   >  1:42694/3‑153 (MQ=255)                                                                                                                                               tatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTa                                                                                 >  1:640464/3‑251 (MQ=255)                                                                                                                                               tatataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGCCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                                                                                                                           >  1:510606/3‑209 (MQ=255)                                                                                                                                                atataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAcc                                                                                                                                                                                          >  1:605674/2‑145 (MQ=255)                                                                                                                                                atataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAg                                                                                <  1:168175/250‑1 (MQ=255)                                                                                                                                                 tataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGGTCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAgg                                                                               <  1:137056/251‑1 (MQ=255)                                                                                                                                                 tataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc                                                                                                                                                                                                        >  1:487734/1‑130 (MQ=255)                                                                                                                                                  ataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGga                                                                              <  1:863514/251‑1 (MQ=255)                                                                                                                                                  ataAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGga                                                                              <  1:475289/251‑1 (MQ=255)                                                                                                                                                       gAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGAc                                                                           <  1:647903/249‑1 (MQ=255)                                                                                                                                                         gTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATc                                                                        >  1:136677/1‑250 (MQ=255)                                                                                                                                                              ggATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTa                                                                                                                                                                        <  1:443323/149‑1 (MQ=255)                                                                                                                                                                            gTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTttat                                                                                                                                           <  1:572207/164‑1 (MQ=255)                                                                                                                                                                             ttgctgccaaaTTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTttcttc                                                   <  1:475889/243‑1 (MQ=255)                                                                                                                                                                                           cAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCaacaac                                     <  1:456074/251‑1 (MQ=255)                                                                                                                                                                                           cAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCaacaac                                     <  1:42284/251‑1 (MQ=255)                                                                                                                                                                                             aaaaaaTAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTg                                   <  1:585112/251‑1 (MQ=255)                                                                                                                                                                                                aaaTAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAAt                                                                   >  1:510171/1‑216 (MQ=255)                                                                                                                                                                                                             cGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGttctttct                                                     >  1:831353/1‑217 (MQ=255)                                                                                                                                                                                                             cGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAaga                   >  1:343210/1‑251 (MQ=255)                                                                                                                                                                                                                   gCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAgg                                                                               >  1:415756/1‑185 (MQ=255)                                                                                                                                                                                                                   gCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCaacaac                                     <  1:465256/227‑1 (MQ=255)                                                                                                                                                                                                                   gCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGagttagt             >  1:795596/1‑251 (MQ=255)                                                                                                                                                                                                                    ccTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGagttagtt            <  1:709211/251‑1 (MQ=255)                                                                                                                                                                                                                        aGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCg        >  1:873763/1‑251 (MQ=255)                                                                                                                                                                                                                         gATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAAc                                                                                                >  1:374747/1‑162 (MQ=255)                                                                                                                                                                                                                             aatcAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATg                                                                                                                 >  1:843870/4‑141 (MQ=255)                                                                                                                                                                                                                              atcAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTAGCaa  >  1:352253/3‑251 (MQ=255)                                                                                                                                                                                                                                  gTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAATGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTAt          >  1:303848/1‑239 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          ATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTGAAGGTATAAACAAAGTTCCTCTTTTAGGAGACATCCCAATTTTAGGTTCTTTCTTCCGTAGTGCAACAACTGAGCGCGATAAAAAAGAGTTAGTTATCGTGGCAA  >  1/1480766‑1481237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑