Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,480,897	A→G	S294S (TCA→TCG)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,480,897	0	A	G	100.0%	184.3 / NA	68	S294S (TCA→TCG)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/34);  total (34/34)

CCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTG  >  1/1480657‑1481132                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             tcACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAAt                                                                                                                                                                                                                                      <  1:568677/248‑1 (MQ=255)    cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat                                                                                                                                                                                                                                 <  1:372500/251‑3 (MQ=255)    cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat                                                                                                                                                                                                                                 <  1:760940/251‑3 (MQ=255)    cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat                                                                                                                                                                                                                                 <  1:464950/251‑3 (MQ=255)      ttGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat                                                                                                                                                                                                                               <  1:13981/251‑1 (MQ=255)        gCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat                                                                                                                                                                                                                                 <  1:226137/247‑3 (MQ=255)        gCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATaa                                                                                                                                                                                                                             >  1:420350/1‑251 (MQ=255)             tAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAg                                                                                                                                                                                                                         <  1:627781/250‑1 (MQ=255)                    ccATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat                                                                                                                                                                                                                               <  1:753350/237‑1 (MQ=255)                    ccATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGAt                                                                                                                                                                                                                 >  1:421541/1‑251 (MQ=255)                           tCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATaaagc                                                                                                                                                                                                            <  1:595627/249‑3 (MQ=255)                                          gTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat                                                                                                                                                                                                                                 <  1:839881/213‑3 (MQ=255)                                                gAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCaa                                                                                                                                                                                     >  1:532332/1‑251 (MQ=255)                                                      gATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAaat                                                                                                                                                                               >  1:2014/1‑251 (MQ=255)                                                       aTTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAaata                                                                                                                                                                              >  1:351871/1‑251 (MQ=255)                                                                aaaTCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTAtt                                                                                                                                                                      >  1:735236/1‑250 (MQ=255)                                                                          gtATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAgg                                                                                                                                                                                                                           <  1:134773/187‑1 (MQ=255)                                                                          gtATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGt                                                                                                                                                                                                     <  1:688489/209‑1 (MQ=255)                                                                              tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       <  1:592527/251‑1 (MQ=255)                                                                              tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       <  1:300619/251‑1 (MQ=255)                                                                              tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       <  1:184271/251‑1 (MQ=255)                                                                               aCGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat                                                                                                                                                                                                                               <  1:754848/178‑1 (MQ=255)                                                                                cGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCa                                                                                                                                                     >  1:869532/1‑251 (MQ=255)                                                                                cGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCa                                                                                                                                                     >  1:514622/1‑251 (MQ=255)                                                                                              aGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcca                                                                                                                                       >  1:685726/1‑249 (MQ=255)                                                                                                   tttCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAg                                                                                                                                                                                                                         >  1:249823/1‑164 (MQ=255)                                                                                                    ttccgcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagctg                                                                                                                                 <  1:419890/244‑9 (MQ=255)                                                                                                       tgcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagctggta                                                                                                                              <  1:716543/247‑12 (MQ=255)                                                                                                        gcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTACTGGCCGAACCGAACATTTCTATGTTGTCAGGTGAAACTGCCGATCTCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAAGTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTACTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGcc                                                                                                                                                             <  1:510225/216‑1 (MQ=255)                                                                                                           tttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       >  1:348614/1‑222 (MQ=255)                                                                                                              attaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATc                                                                                                                                                      <  1:303161/220‑1 (MQ=255)                                                                                                              attaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATaa                                                                                                                       >  1:2316/1‑251 (MQ=255)                                                                                                               ttaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       >  1:398208/1‑218 (MQ=255)                                                                                                                    gccttAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       >  1:17872/4‑213 (MQ=255)                                                                                                                             aaCCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagc                                                                                                                                   <  1:791753/224‑7 (MQ=255)                                                                                                                              aCCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCggg                                                                                                      >  1:522610/1‑251 (MQ=255)                                                                                                                                 aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat                                                                                                   <  1:203713/251‑1 (MQ=255)                                                                                                                                 aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat                                                                                                   <  1:33771/251‑1 (MQ=255)                                                                                                                                    gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt                                                                                                                                                       >  1:637786/1‑197 (MQ=255)                                                                                                                                    gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATAtt                                                                                                >  1:183680/1‑251 (MQ=255)                                                                                                                                      aaTGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc                                                                                               >  1:54873/1‑250 (MQ=255)                                                                                                                                          gTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc                                                                                               >  1:610723/1‑246 (MQ=255)                                                                                                                                               gTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcc                                                                                                                                        >  1:269539/1‑200 (MQ=255)                                                                                                                                               gTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCttt                                                                                       <  1:858954/249‑1 (MQ=255)                                                                                                                                                  cTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAc                                                                                  <  1:132618/251‑1 (MQ=255)                                                                                                                                                           ccgaacATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGc                                                                         <  1:503844/251‑1 (MQ=255)                                                                                                                                                               acATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcc                                                                                                                                        >  1:582482/1‑184 (MQ=255)                                                                                                                                                                  tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAACATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                    >  1:619916/1‑247 (MQ=255)                                                                                                                                                                  tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccat                                                                                                                                      <  1:580253/184‑4 (MQ=255)                                                                                                                                                                  tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAAc                                                                                                                      >  1:591550/1‑200 (MQ=255)                                                                                                                                                                  tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                    >  1:42944/1‑247 (MQ=255)                                                                                                                                                                  tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGA‑GGAAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt                                                                    >  1:699856/1‑247 (MQ=255)                                                                                                                                                                            atcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAcc                                                                                 <  1:385210/222‑1 (MQ=255)                                                                                                                                                                             tcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGaagt                                                       <  1:458771/248‑4 (MQ=255)                                                                                                                                                                              caggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTa                                                      >  1:299047/3‑251 (MQ=255)                                                                                                                                                                                             atatCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTa                                                                                                                                              <  1:781322/149‑1 (MQ=255)                                                                                                                                                                                               atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt                                     >  1:672845/1‑251 (MQ=255)                                                                                                                                                                                               atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt                                     >  1:537119/1‑251 (MQ=255)                                                                                                                                                                                                  ttAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTttat                                  <  1:572700/251‑1 (MQ=255)                                                                                                                                                                                                                aaTCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGC‑AAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCt                                                                  <  1:213319/204‑1 (MQ=255)                                                                                                                                                                                                                 aTCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATAT‑TAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                  <  1:610478/251‑1 (MQ=255)                                                                                                                                                                                                                  tCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                  <  1:298449/251‑1 (MQ=255)                                                                                                                                                                                                                     cATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCaatca                                                                                                                                                 >  1:489199/1‑119 (MQ=255)                                                                                                                                                                                                                     cATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAg                >  1:686939/1‑250 (MQ=255)                                                                                                                                                                                                                                 gagATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTAtt                                                             >  1:302300/3‑193 (MQ=255)                                                                                                                                                                                                                                  agATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTg  >  1:260867/2‑251 (MQ=255)                                                                                                                                                                                                                                  agATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTg  >  1:624027/2‑251 (MQ=255)                                                                                                                                                                                                                                           tGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTc                  >  1:373521/1‑226 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             CCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTATTATCGGTGGCTTATTCAGCTCAAATGACCTTG  >  1/1480657‑1481132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑