| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,480,858 | G→A | G281G (GGG→GGA) | unknown → | Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,480,858 | 0 | G | A | 100.0% | 190.7 / NA | 71 | G281G (GGG→GGA) | unknown | Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/39); total (32/39) | |||||||||||
GGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTAT > 1/1480615‑1481100 | ggTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTGTTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGaaa > 1:630824/1‑251 (MQ=255) gTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGaaa < 1:558930/250‑1 (MQ=255) aaataagAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATcc < 1:415451/251‑1 (MQ=255) ataagAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCa > 1:687412/1‑251 (MQ=255) aagaaaCTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCAtt < 1:838673/248‑1 (MQ=255) ttCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGcacaac < 1:501298/251‑3 (MQ=255) gcgaTGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGcacaacgagat < 1:502991/246‑8 (MQ=255) tGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGAt < 1:403054/251‑1 (MQ=255) gtaTTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGcac < 1:474023/232‑1 (MQ=255) gtaTTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGt > 1:195924/3‑251 (MQ=255) aaCTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGcac < 1:644635/229‑1 (MQ=255) tcACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAAt < 1:568677/248‑1 (MQ=255) cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat < 1:760940/251‑3 (MQ=255) cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat < 1:464950/251‑3 (MQ=255) cGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat < 1:372500/251‑3 (MQ=255) ttGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat < 1:13981/251‑1 (MQ=255) gCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat < 1:226137/247‑3 (MQ=255) gCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATaa > 1:420350/1‑251 (MQ=255) tAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAg < 1:627781/250‑1 (MQ=255) ccATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat < 1:753350/237‑1 (MQ=255) ccATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGAt > 1:421541/1‑251 (MQ=255) tCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATaaagc < 1:595627/249‑3 (MQ=255) gTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTAtat < 1:839881/213‑3 (MQ=255) gAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCaa > 1:532332/1‑251 (MQ=255) gATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAaat > 1:2014/1‑251 (MQ=255) aTTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAaata > 1:351871/1‑251 (MQ=255) aaaTCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTAtt > 1:735236/1‑250 (MQ=255) gtATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAgg < 1:134773/187‑1 (MQ=255) gtATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGt < 1:688489/209‑1 (MQ=255) tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt < 1:592527/251‑1 (MQ=255) tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt < 1:300619/251‑1 (MQ=255) tACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt < 1:184271/251‑1 (MQ=255) aCGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTatatat < 1:754848/178‑1 (MQ=255) cGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCa > 1:869532/1‑251 (MQ=255) cGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCa > 1:514622/1‑251 (MQ=255) aGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcca > 1:685726/1‑249 (MQ=255) tttCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAg > 1:249823/1‑164 (MQ=255) ttccgcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagctg < 1:419890/244‑9 (MQ=255) tgcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagctggta < 1:716543/247‑12 (MQ=255) gcgtttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTACTGGCCGAACCGAACATTTCTATGTTGTCAGGTGAAACTGCCGATCTCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAAGTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTACTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGcc < 1:510225/216‑1 (MQ=255) tttATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt > 1:348614/1‑222 (MQ=255) attaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATc < 1:303161/220‑1 (MQ=255) attaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATaa > 1:2316/1‑251 (MQ=255) ttaATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt > 1:398208/1‑218 (MQ=255) gccttAGATAACCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt > 1:17872/4‑213 (MQ=255) aaCCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccatagc < 1:791753/224‑7 (MQ=255) aCCAAAGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCggg > 1:522610/1‑251 (MQ=255) aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat < 1:203713/251‑1 (MQ=255) aaaGTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAat < 1:33771/251‑1 (MQ=255) gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGcac > 1:325592/1‑90 (MQ=255) gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGAt > 1:637786/1‑197 (MQ=255) gTAATGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATAtt > 1:183680/1‑251 (MQ=255) aaTGGTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc > 1:54873/1‑250 (MQ=255) gTAAGGTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTc > 1:610723/1‑246 (MQ=255) gTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcc > 1:269539/1‑200 (MQ=255) gTTCTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCttt < 1:858954/249‑1 (MQ=255) cTGGCCGAACCGAACATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAc < 1:132618/251‑1 (MQ=255) ccgaacATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGc < 1:503844/251‑1 (MQ=255) acATTTCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAcc > 1:582482/1‑184 (MQ=255) tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAACATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt > 1:619916/1‑247 (MQ=255) tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCAccat < 1:580253/184‑4 (MQ=255) tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAAc > 1:591550/1‑200 (MQ=255) tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt > 1:42944/1‑247 (MQ=255) tttCTATGTTATCAGGTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGA‑GGAAGTCGGGAATATTCCGTTCTTTAATACCCGTAAAGCaaagt > 1:699856/1‑247 (MQ=255) atcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATAcc < 1:385210/222‑1 (MQ=255) tcaggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGaagt < 1:458771/248‑4 (MQ=255) caggTGAAACTGCCGATATCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTa > 1:299047/3‑251 (MQ=255) atatCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTa < 1:781322/149‑1 (MQ=255) atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt > 1:672845/1‑251 (MQ=255) atCTTAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGttt > 1:537119/1‑251 (MQ=255) ttAGTGGGAGGTGAAATCCCATTTGCACAACGAGATAAAGATGGTTCGCCAACAATTATATATAAGGAGTTTGGGATAAAGCTCGCTGTTGCTGCCAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAATCAGTAAGCACCATAGCTGGTAACTATAACTTTGAGGGAG‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAGTCTTTATTTGAAGTAGCTAACGGAGAAAGTTttat < 1:572700/251‑1 (MQ=255) | GGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCTATGTTATCCGGTGAAACTGCCGATATCTTAGTGGGGGGAGAAATCCCATTTGCACAGCGGGATAGAGATGGTTCACCAACAATTATCTATAAGGAGTTTGGGATAAAACTCGCTGTTGCGGCTAAATTGCAAAAAAATAATCGTATTCGTTTAGCCTTAGATCAAACAGTAAGCACTATTGCAGGTAACTATAACTTTGATGGAA‑TCGGGAATATTCCGTTCTTTAATACCCGTAAAGCAAAATCTTTATTTGAGGTAGCTAACGGAGAAAGTTTTAT > 1/1480615‑1481100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |