Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,480,576	C→A	A187A (GCC→GCA)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,480,576	0	C	A	100.0%	204.5 / NA	77	A187A (GCC→GCA)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/40);  total (37/40)

TTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCT  >  1/1480361‑1480822                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       tttccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttgt                                                                                                                                                                                                                     >  1:567193/4‑251 (MQ=255)   tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                                                                                                                                   <  1:735964/250‑1 (MQ=255)   tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                                                                                                                                   <  1:591569/250‑1 (MQ=255)   tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                                                                                                                                   <  1:742506/250‑1 (MQ=255)     cAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTagag                                                                                                                                                                                                                 <  1:251446/251‑1 (MQ=255)        aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                                                                                                                                                                                                              >  1:795982/1‑251 (MQ=255)        aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                                                                                                                                                                                                              >  1:862888/1‑251 (MQ=255)           cTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGtaaaa                                                                                                                                                                                                           >  1:837636/1‑250 (MQ=255)                  atctGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATaagaaa                                                                                                                                                                                                     >  1:176042/1‑250 (MQ=255)                      gtgaagaaaGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACttt                                                                                                                                                                                                 >  1:216054/7‑250 (MQ=255)                               gTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGcgat                                                                                                                                                                                       >  1:244491/1‑248 (MQ=255)                                  ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttg                                                                                                                                                                                                                      <  1:546111/214‑1 (MQ=255)                                  ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATggg                                                                                                                                                                                    >  1:781966/4‑251 (MQ=255)                                       ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                                                                                                                               >  1:894327/5‑251 (MQ=255)                                       ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                                                                                                                               >  1:340674/5‑251 (MQ=255)                                       ggcctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGCTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTa                                                                                                                                                                               >  1:653579/5‑251 (MQ=255)                                         cctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAAc                                                                                                                                                                             <  1:226130/249‑1 (MQ=255)                                         cctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAAc                                                                                                                                                                             <  1:117591/249‑1 (MQ=255)                                          ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:237683/250‑1 (MQ=255)                                          ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:878691/250‑1 (MQ=255)                                          ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:159963/250‑1 (MQ=255)                                          ctATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:806362/250‑1 (MQ=255)                                            aTGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTgg                                                                                                                                                                          >  1:17735/1‑251 (MQ=255)                                            aTGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTgg                                                                                                                                                                          >  1:769744/1‑251 (MQ=255)                                               tAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAg                                                                                                                                                                        <  1:548782/250‑1 (MQ=255)                                                   tgaaggtaaaGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTaaagaacggaata                                                                                                                                                                   <  1:175643/244‑12 (MQ=255)                                                     aaggtaaaGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGt                                                                                                                                                                  <  1:766454/245‑1 (MQ=255)                                                                    cacAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            >  1:40864/3‑225 (MQ=255)                                                                      cAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCAtt                                                                                                                                                >  1:686430/1‑251 (MQ=255)                                                                         gaagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                                                                                                                                                                                                   >  1:361749/1‑181 (MQ=255)                                                                         gaagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGt                                                                                                                                             >  1:481702/1‑251 (MQ=255)                                                                          aagaaAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAg                                                                                                                                              <  1:494294/249‑1 (MQ=255)                                                                                gCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGt                                                                                                                                      >  1:276557/1‑251 (MQ=255)                                                                                          tcgtaGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:391127/199‑1 (MQ=255)                                                                                          tcgtaGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGtt                                                                                                                            >  1:647886/5‑251 (MQ=255)                                                                                               gAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGa                                                                                                                       >  1:82274/1‑251 (MQ=255)                                                                                                           aaGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAAt                                                                                                           >  1:412058/1‑251 (MQ=255)                                                                                                             gCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCAt                                                                                                                                                 >  1:305182/1‑211 (MQ=255)                                                                                                             gCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATgg                                                                                                         >  1:225218/1‑251 (MQ=255)                                                                                                                ttAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGaaa                                                                                                      >  1:430707/1‑251 (MQ=255)                                                                                                                    gaaacggtcgAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATc                                                                                                    <  1:808615/240‑1 (MQ=255)                                                                                                                        cggtcgAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGgtg                                                                                              <  1:322667/246‑1 (MQ=255)                                                                                                                          gtcgAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            >  1:175589/4‑171 (MQ=255)                                                                                                                          gtcgAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTa                                                                                            >  1:227691/4‑251 (MQ=255)                                                                                                                               aaGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGACACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACg                                                                                       <  1:92207/251‑1 (MQ=255)                                                                                                                                  gTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGt                                                                                      <  1:341443/249‑1 (MQ=255)                                                                                                                                        gAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAg                                                                                                                                              >  1:804591/1‑187 (MQ=255)                                                                                                                                                    ggggaagatgctttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTAttt                                                                   >  1:527983/19‑250 (MQ=255)                                                                                                                                                     gggaagatgctttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTc                                                                  <  1:628113/233‑1 (MQ=255)                                                                                                                                                           atgctttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTgg                                                                                                                                                                          <  1:121394/129‑1 (MQ=255)                                                                                                                                                             gctttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTttat                                                         >  1:142977/10‑251 (MQ=255)                                                                                                                                                                ttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTAc                                                                                        >  1:41348/7‑217 (MQ=255)                                                                                                                                                                ttgccgttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTaa                                                      <  1:272800/245‑1 (MQ=255)                                                                                                                                                                      ttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACt                                                                                                                                                                            <  1:83425/127‑1 (MQ=255)                                                                                                                                                                      ttCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTg                                                                                                                                                                           >  1:604360/1‑128 (MQ=255)                                                                                                                                                                        cTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAg                                              <  1:653987/251‑1 (MQ=255)                                                                                                                                                                            gACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTttatt                                                        >  1:66897/1‑237 (MQ=255)                                                                                                                                                                                  taccATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAg                                    >  1:751663/4‑251 (MQ=255)                                                                                                                                                                                    ccATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAg                                                                                                                                              <  1:596387/142‑1 (MQ=255)                                                                                                                                                                                    ccATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTa                                                                      <  1:802641/214‑1 (MQ=255)                                                                                                                                                                                          cGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTa                            <  1:24880/251‑1 (MQ=255)                                                                                                                                                                                          cGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTa                            <  1:246843/251‑1 (MQ=255)                                                                                                                                                                                            aTGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTa                            <  1:47155/249‑1 (MQ=255)                                                                                                                                                                                               gtgtTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTg                                                                <  1:897524/210‑1 (MQ=255)                                                                                                                                                                                               gtgtTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCa                                       <  1:683427/235‑1 (MQ=255)                                                                                                                                                                                               gtgtTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGtt                       >  1:764481/1‑251 (MQ=255)                                                                                                                                                                                               gtgtTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGtt                       >  1:591270/1‑251 (MQ=255)                                                                                                                                                                                                 gttgTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCTATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAAt                                                                                                           <  1:363553/162‑1 (MQ=255)                                                                                                                                                                                                 gttgTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGt                                                                                      <  1:259762/183‑1 (MQ=255)                                                                                                                                                                                                 gttgTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAgg                                                                        <  1:412657/197‑1 (MQ=255)                                                                                                                                                                                                   tgTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGac                                                                                >  1:841814/2‑189 (MQ=255)                                                                                                                                                                                                     ttGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGcc                 <  1:520483/251‑1 (MQ=255)                                                                                                                                                                                                     ttGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGcc                 <  1:612325/251‑1 (MQ=255)                                                                                                                                                                                                            cGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGa                                                                                                                                         <  1:843136/124‑1 (MQ=255)                                                                                                                                                                                                            cGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGACGCAAAAGGTATTTCTGCGTTTATTAATGCCTTAGATAACCAAAGTAATGGTAAGGTTCTGGc                  <  1:517859/243‑1 (MQ=255)                                                                                                                                                                                                              cAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGt                                                                                      <  1:254779/173‑1 (MQ=255)                                                                                                                                                                                                                   atTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATGGGTATTAACTGGAGTCACGTTGCCGGTAGTGGTCCA‑TAGTCGGAGGTAACTTCGGTTTTGGAGGGGGATTCAATGGAAATTAAGGGGTATTACGTTTAGACGCAAAAGGTATTTTTGCGTTTATTAATGCCTTAGATTAACAAAGTAATGGTAAGGTTCTGGCCGAACCGAACAttttt  >  1:870228/1‑249 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGGCATTAACTGGAGCCACGTTGCCGGTAGTGGTCCATTAGTCGGAGGTAACTTCGGTTTCGGAGGTGGATTCAATGGAAATCAAGGTGTATTACGTTTAGATGCGAAAGGTATTTCTGCCTTTATTAATGCTTTAGATAACCAAAGTAATGGTAAAGTTCTGGCCGAACCGAACATTTCT  >  1/1480361‑1480822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑