Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,480,402	T→C	A129A (GCT→GCC)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,480,402	0	T	C	100.0%	106.6 / NA	38	A129A (GCT→GCC)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/21);  total (17/21)

ATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGG  >  1/1480157‑1480644                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                                               <  1:737393/251‑1 (MQ=255) aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                                               <  1:641929/251‑1 (MQ=255) aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                                               <  1:368532/251‑1 (MQ=255) aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                                               <  1:580437/251‑1 (MQ=255)      cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          <  1:873240/251‑1 (MQ=255)      cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          <  1:682816/251‑1 (MQ=255)      cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          <  1:639839/251‑1 (MQ=255)      cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          <  1:554590/251‑1 (MQ=255)          aTTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                                               <  1:673619/242‑1 (MQ=255)                       cACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTtca                                                                                                                                                                                                                         >  1:75060/1‑250 (MQ=255)                                                  tAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGa                                                                                                                                                                                              <  1:144644/251‑1 (MQ=255)                                                            cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTc                                                                                                                                                                                                   <  1:244589/236‑1 (MQ=255)                                                            cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGgag                                                                                                                                                                                    >  1:605228/1‑251 (MQ=255)                                                            cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGgag                                                                                                                                                                                    >  1:597552/1‑251 (MQ=255)                                                                tttATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTaa                                                                                                                                                                                                                                             >  1:337503/1‑190 (MQ=255)                                                                tttATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAg                                                                                                                                                                                 >  1:703990/1‑250 (MQ=255)                                                                             cAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCt                                                                                                                                                                               >  1:676765/1‑239 (MQ=255)                                                                                  gaggaAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGaaggt                                                                                                                                                                                                                                     <  1:224996/180‑5 (MQ=255)                                                                                         gTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          >  1:519009/1‑168 (MQ=255)                                                                                         gTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTa                                                                                                                                                         >  1:726356/1‑249 (MQ=255)                                                                                            gAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGaa                                                                                                                                                    >  1:818469/1‑251 (MQ=255)                                                                                             aaCAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGa                                                                                                                                                     <  1:80305/249‑1 (MQ=255)                                                                                              aCAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGa                                                                                                                                                                         <  1:395681/228‑1 (MQ=255)                                                                                               cAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                                          <  1:455650/162‑1 (MQ=255)                                                                                                 gAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGa                                                                                                                                                                         <  1:556071/225‑1 (MQ=255)                                                                                                                                                                                                             tttccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttgt                                   >  1:567193/4‑251 (MQ=255)                                                                                                                                                                                                               tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                 <  1:735964/250‑1 (MQ=255)                                                                                                                                                                                                               tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                 <  1:742506/250‑1 (MQ=255)                                                                                                                                                                                                               tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                                 <  1:591569/250‑1 (MQ=255)                                                                                                                                                                                                                 cAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTagag                               <  1:251446/251‑1 (MQ=255)                                                                                                                                                                                                                    aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                            >  1:795982/1‑251 (MQ=255)                                                                                                                                                                                                                    aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta                            >  1:862888/1‑251 (MQ=255)                                                                                                                                                                                                                       cTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGtaaaa                         >  1:837636/1‑250 (MQ=255)                                                                                                                                                                                                                              atctGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATaagaaa                   >  1:176042/1‑250 (MQ=255)                                                                                                                                                                                                                                  gtgaagaaaGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACttt               >  1:216054/7‑250 (MQ=255)                                                                                                                                                                                                                                           gTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGcgat     >  1:244491/1‑248 (MQ=255)                                                                                                                                                                                                                                              ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttg                                    <  1:546111/214‑1 (MQ=255)                                                                                                                                                                                                                                              ggtaaGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAGAAACTTTCTGAGGCGATggg  >  1:781966/4‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAACTTTCTGAGGCAATGGG  >  1/1480157‑1480644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑