Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,480,384	A→G	V123V (GTA→GTG)	unknown →	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,480,384	0	A	G	100.0%	120.4 / NA	43	V123V (GTA→GTG)	unknown	Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/26);  total (17/26)

CAAACAAAAGAAAAGATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAA  >  1/1480142‑1480627                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    caaacaaaAGAAAAGATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGt                                                                                                                                                                                                                                             <  1:230276/251‑1 (MQ=255)      aaaagaaaagATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAggta                                                                                                                                                                                                                                        <  1:391479/251‑3 (MQ=255)           aaaagaTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGcc                                                                                                                                                                                                                                   <  1:226588/251‑2 (MQ=255)           aaaagaTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGc                                                                                                                                                                                                                                    <  1:321199/250‑1 (MQ=255)                aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                              <  1:737393/251‑1 (MQ=255)                aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                              <  1:641929/251‑1 (MQ=255)                aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                              <  1:580437/251‑1 (MQ=255)                aTCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                              <  1:368532/251‑1 (MQ=255)                     cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         <  1:873240/251‑1 (MQ=255)                     cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         <  1:682816/251‑1 (MQ=255)                     cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         <  1:639839/251‑1 (MQ=255)                     cacTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         <  1:554590/251‑1 (MQ=255)                         aTTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGt                                                                                                                                                                                                                              <  1:673619/242‑1 (MQ=255)                                      cACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGcc                                                                                                                                                                                                                                   >  1:259137/1‑223 (MQ=255)                                      cACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTtca                                                                                                                                                                                                        >  1:75060/1‑250 (MQ=255)                                          aaaTATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGcc                                                                                                                                                                                                                                   >  1:266301/1‑219 (MQ=255)                                            atatTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGcc                                                                                                                                                                                                                                   <  1:296150/218‑2 (MQ=255)                                                                 tAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGa                                                                                                                                                                             <  1:144644/251‑1 (MQ=255)                                                                           cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTc                                                                                                                                                                                  <  1:244589/236‑1 (MQ=255)                                                                           cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGgag                                                                                                                                                                   >  1:597552/1‑251 (MQ=255)                                                                           cacCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGgag                                                                                                                                                                   >  1:605228/1‑251 (MQ=255)                                                                               tttATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTaa                                                                                                                                                                                                                            >  1:337503/1‑190 (MQ=255)                                                                               tttATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAg                                                                                                                                                                >  1:703990/1‑250 (MQ=255)                                                                                            cAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCt                                                                                                                                                              >  1:676765/1‑239 (MQ=255)                                                                                                 gaggaAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGaaggt                                                                                                                                                                                                                    <  1:224996/180‑5 (MQ=255)                                                                                                        gTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         >  1:519009/1‑168 (MQ=255)                                                                                                        gTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTa                                                                                                                                        >  1:726356/1‑249 (MQ=255)                                                                                                          aGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGcc                                                                                                                                                                                                                                   <  1:658551/156‑2 (MQ=255)                                                                                                           gAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGc                                                                                                                                                                                                                                    >  1:112598/1‑154 (MQ=255)                                                                                                           gAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGaa                                                                                                                                   >  1:818469/1‑251 (MQ=255)                                                                                                            aaCAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGa                                                                                                                                    <  1:80305/249‑1 (MQ=255)                                                                                                             aCAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGa                                                                                                                                                        <  1:395681/228‑1 (MQ=255)                                                                                                              cAGAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTg                                                                                                                                                                                                                         <  1:455650/162‑1 (MQ=255)                                                                                                                gAAGTTACTGCTTTCGGAGCAGATGGTCGGCCTTTGACGTCTGATACCGTTAATGTTGATTCTGTTGTCACCAGCATTGCAGATACGAATAAACAACTGAAGAGCCATTTTCCAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGa                                                                                                                                                        <  1:556071/225‑1 (MQ=255)                                                                                                                                                                                                                            tttccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCtgttgt                  >  1:567193/4‑251 (MQ=255)                                                                                                                                                                                                                              tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                <  1:591569/250‑1 (MQ=255)                                                                                                                                                                                                                              tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                <  1:735964/250‑1 (MQ=255)                                                                                                                                                                                                                              tccAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTag                <  1:742506/250‑1 (MQ=255)                                                                                                                                                                                                                                cAAATACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTagag              <  1:251446/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta           >  1:795982/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTACTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGta           >  1:862888/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTAATCTATCTGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGtaaaa        >  1:837636/1‑250 (MQ=255)                                                                                                                                                                                                                                             atctGTGAAGAAAGTAGGTAAGGCCTATGTAATTGAAGGTAAAGCTCGTTCACAAGAAGAAAGCGATGAAGTTCGTAGAATTGTAGGAGAAGCTTTAGGAAACGGTCGAAAGGTTACTGAAACAAAGTTAGGGGAAGATGCTTTGCCGTTCTTAGACAAATACCATTACGATGGTGTTGTTGATAACGCAAATATTGCAGACACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATaagaaa  >  1:176042/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CAAACAAAAGAAAAGATCGACACTATTTTCGTCTCTTCACCAAATATTGCCGACTATGAAATTTTAGACGACAACACCTTTATTATCTACGCAAAGGAGGAAGGTAGAACAGAAGTTACTGCTTTCGGAGCAGATGGTCGTCCCTTAACATCCGATACTGTTAATGTTGATTCTGTTGTGACCAGTATTGCAGACACGAATAAACAACTTAAAAGCCGTTTCCCAAATACTAATCTATCTGTAAAAAAAGTAGGCAAGGCTTATGTAATTGAGGGAAAAGCCCGTTCTCAAGAAGAAAGCGATGAGGTTCGCAGAATTGTAGGAGAAGCTTTAGGAAGTGGTAGAAAGGTTACTGAAACAAAATTAGGAGAAGACTCTTTACCATTCTTAGACAAATATCATTACGATGGTGTCGTTGATAACGCAAATATTGCCGATACAACCCAAATTAATGTGAAGCTTTCTGTTGTAGAGGTAAATAAAAAA  >  1/1480142‑1480627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑