Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	231,601	A→G	T69A (ACG→GCG)	unknown →	Membrane protein insertion efficiency factor YidD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	231,601	0	A	G	100.0%	180.0 / NA	64	T69A (ACG→GCG)	unknown	Membrane protein insertion efficiency factor YidD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/41);  total (23/41)

GTGTTTTAAACAACCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATACGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTATTGCAACGGA  >  1/231354‑231835                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gtgtTTTAAACAACCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTAAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCgg                                                                                                                                                                                                                                         >  1:440217/1‑251 (MQ=255)  tgtTTTAAACAACCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCggg                                                                                                                                                                                                                                        <  1:481800/251‑1 (MQ=255)           caacCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGa                                                                                                                                                                                                                               >  1:390436/1‑251 (MQ=255)            aacCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGAt                                                                                                                                                                                                                              <  1:11185/251‑1 (MQ=255)            aacCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGAt                                                                                                                                                                                                                              <  1:756651/251‑1 (MQ=255)              ccGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATcc                                                                                                                                                                                                                            <  1:892236/251‑1 (MQ=255)                   cTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGtt                                                                                                                                                                                                                        >  1:856593/1‑250 (MQ=255)                       aTGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCAcc                                                                                                                                                                                                                   <  1:365708/251‑1 (MQ=255)                        tGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCg                                                                                                                                                                                                                  <  1:249780/251‑1 (MQ=255)                         ggCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGa                                                                                                                                                                                                                 >  1:667869/1‑251 (MQ=255)                              aCGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATc                                                                                                                                                                                                            <  1:483719/251‑1 (MQ=255)                                 cTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCaat                                                                                                                                                                                                         <  1:170316/251‑1 (MQ=255)                                   ggATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCgggtggg                                                                                                                                                                                                                                    <  1:166708/222‑1 (MQ=255)                                   ggATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCgggtggg                                                                                                                                                                                                                                    <  1:719633/222‑1 (MQ=255)                                    gATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCaataa                                                                                                                                                                                                       <  1:819988/250‑1 (MQ=255)                                     aTAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAataata                                                                                                                                                                                                     >  1:522452/1‑251 (MQ=255)                                        aaTTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACa                                                                                                                                                                                                  >  1:117333/1‑251 (MQ=255)                                            aTGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAAc                                                                                                                                                                                              <  1:118002/251‑1 (MQ=255)                                               gCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACag                                                                                                                                                                                            <  1:123856/250‑1 (MQ=255)                                                     acacATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaataa                                                                                                                                                                                     >  1:712469/1‑251 (MQ=255)                                                       acaTTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaataaat                                                                                                                                                                                   <  1:163851/251‑1 (MQ=255)                                                        caTTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaataaata                                                                                                                                                                                  >  1:287628/1‑251 (MQ=255)                                                            cGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATgg                                                                                                                                                                              <  1:241240/251‑1 (MQ=255)                                                              tttAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGAt                                                                                                                                                                            <  1:451074/251‑1 (MQ=255)                                                                      aaaaaTCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACg                                                                                                                                                                    <  1:726748/251‑1 (MQ=255)                                                                              tCATCGGATTAATTCGTTTTTATCAAATAGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCctgc                                                                                                                                                            <  1:45958/251‑1 (MQ=255)                                                                                    gATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaataaata                                                                                                                                                                                  >  1:655920/1‑223 (MQ=255)                                                                                    gATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTc                                                                                                                                                       <  1:328296/250‑1 (MQ=255)                                                                                                    tCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTAt                                                                                                                                      <  1:292518/251‑1 (MQ=255)                                                                                                    tCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTAt                                                                                                                                      <  1:585797/251‑1 (MQ=255)                                                                                                       aaTCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGAt                                                                                                                                                                                                             <  1:123046/177‑1 (MQ=255)                                                                                                        aTCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCAt                                                                                                                                                     >  1:392410/1‑232 (MQ=255)                                                                                                        aTCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGt                                                                                                                                    >  1:264502/1‑249 (MQ=255)                                                                                                           gTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGCGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGCGGGAACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCttt                                                                                                                                        >  1:733750/1‑242 (MQ=255)                                                                                                            ttATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTa                                                                                                                              >  1:609693/1‑251 (MQ=255)                                                                                                                  gCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaat                                                                                                                                                                                       <  1:239262/188‑1 (MQ=255)                                                                                                                  gCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGAt                                                                                                                                                                            >  1:215115/1‑199 (MQ=255)                                                                                                                  gCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCac                                                                                                                                                <  1:881256/227‑1 (MQ=255)                                                                                                                   cccGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGt                                                                                                                          <  1:797922/248‑1 (MQ=255)                                                                                                                       ttAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAg                                                                                                                   <  1:226647/251‑1 (MQ=255)                                                                                                                               tCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGc                                                                                                            <  1:353845/250‑1 (MQ=255)                                                                                                                                          gTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAg                                                                                                                                                                       <  1:500359/180‑1 (MQ=255)                                                                                                                                           ttttACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATca                                                                                               <  1:328600/251‑1 (MQ=255)                                                                                                                                                  cccACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGcc                                                                                         <  1:264911/250‑1 (MQ=255)                                                                                                                                                    cACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCaaa                                                                                      >  1:655399/1‑251 (MQ=255)                                                                                                                                                      cGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCaaaa                                                                                     >  1:689663/1‑250 (MQ=255)                                                                                                                                                       gtgtTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAAc                                                                                    >  1:75728/1‑250 (MQ=255)                                                                                                                                                         gtTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATcc                                                                                                                                                                                                                            <  1:372478/112‑1 (MQ=255)                                                                                                                                                         gtTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATca                                                                                               <  1:340509/237‑1 (MQ=255)                                                                                                                                                             cTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAATGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGaataaat                                                                                                                                                                                   <  1:842929/149‑1 (MQ=255)                                                                                                                                                               gTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGt                                                                                                                                    >  1:232438/1‑194 (MQ=255)                                                                                                                                                                                                cgCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATcac                                                                                              <  1:223375/199‑1 (MQ=255)                                                                                                                                                                                                cgCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTAc                                          <  1:831418/251‑1 (MQ=255)                                                                                                                                                                                                  cTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATcac                                                                                              <  1:783583/197‑1 (MQ=255)                                                                                                                                                                                                          tggtTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTAAAATGCGGGTGGGTAAGATCCCGTTCCACCGAAAATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTCCTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACACCCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTc                                <  1:247120/251‑1 (MQ=255)                                                                                                                                                                                                            gtTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAACCCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTcc                               <  1:185605/250‑1 (MQ=255)                                                                                                                                                                                                                     cGCTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTa                     >  1:31454/1‑251 (MQ=255)                                                                                                                                                                                                                       cTTAAACGTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTg                                                                                                       <  1:331311/167‑1 (MQ=255)                                                                                                                                                                                                                              gTATATTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTa            >  1:842513/1‑251 (MQ=255)                                                                                                                                                                                                                                  atTAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTATTGc        <  1:359596/251‑1 (MQ=255)                                                                                                                                                                                                                                   ttAAAATGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTATTGCa       >  1:357968/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGTCATCCTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTATTGCAACGGa  >  1:393625/1‑251 (MQ=255)                                                                                                                                                                                                                                                cTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTc                                                                                                                                   >  1:720213/1‑114 (MQ=255)                                                                                                                                                                                                                                                cTTTAAATGCGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTc                                                                                                                                   <  1:283200/114‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GTGTTTTAAACAACCGCACTTTATGGCAAACGCTGGATAAATTATGGCAACGACACATTCGTTTAGCGCAAAAATCCTCATCGGATTAATTCGTTTTTATCAAATCGTTATCAGCCCGTTAATCGGTCCGCGTTGCCGTTTTACGCCCACGTGTTCCTGTTATGGCATTGAGGCGGTAAAAACGCACGGCACGCTAAAAGGTGGTTGGCTTACGCTTAAACGTATATTAAAATGTCATCCTTTAAATACGGGTGGGTACGATCCCGTTCCACCGAAGATCAATAATAACAAAACAGAGAATAAATAATGGATTCAAGACGAGGCCTGCTGGTCATTGCACTACTCTTTATGTCTTTTATTGTTTATCAGCAATGGCAACTGGATTATCACACGCCAAAACCTGTAACAACCGAACAAACCGCCGCATCGGCATCCGATGTACCTGCCTCTTCCAATGCGGGTAATGCGGTTATTGCAACGGA  >  1/231354‑231835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑