Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,479,751	A→G	I188V (ATT→GTT)	unknown →	Flp pilus assembly protein RcpC/CpaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,479,751	0	A	G	100.0%	119.4 / NA	42	I188V (ATT→GTT)	unknown	Flp pilus assembly protein RcpC/CpaB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/13);  total (29/13)

CTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAACGATCCTAGATTCTTTACATCCAGCCTAGATCCGAATCAAGAAGTTGCATATCGCATCTATGTTAAATCTGGTGATGATTACATTCTAAATACTATTTTAAGTGGAGACTATGTTGCTATCTATAGCCAACAAATTGCTTCAGATTCGCGCAACACTTATGATCGTAAAGATTTAGTTAAAATTTTAGGTAAAGTATTGGTTTTACAAGCAAGAAACTTTACTCAATCAACAGAACAAGGTGCTCCTGAAGCTGAAAAGGCAGCA‑GA‑CGGGATTCCAAACAATCAAGAATATGCTGGTTATGTAGCACTAAAAATAACTGCTGAACAAGCAAAAAAATTCTATTCACTGGATAGAGAATCAAAGTTAGTTATATTACCTGCCGAAAATAATACTCAAGAAGTTGATTCA  >  1/1479504‑1479978                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      cTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGttt                                                                                                                                                                                                                                    >  1:263498/1‑251 (MQ=255)   aTTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTa                                                                                                                                                                                                                                  >  1:105407/1‑251 (MQ=255)    tttAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAg                                                                                                                                                                                                                                 <  1:35820/251‑1 (MQ=255)            cAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggc                                                                                                                                                                                                                          <  1:366099/250‑4 (MQ=255)                  aTGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggcagtggtt                                                                                                                                                                                                                   >  1:605186/1‑241 (MQ=255)                             ggTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacg                                                                                                                                                                                                        >  1:412097/1‑249 (MQ=255)                             ggTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaag                                                                                                                                                                                                            >  1:141345/1‑247 (MQ=255)                              gTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacgg                                                                                                                                                                                                       >  1:682175/1‑248 (MQ=255)                                   tttCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattt                                                                                                                                                                                                  >  1:564018/1‑243 (MQ=255)                                           caacaaATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCTTATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggc                                                                                                                                                                                                                          <  1:841199/219‑4 (MQ=255)                                                aaTCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggcagtggtt                                                                                                                                                                                                                   >  1:716179/1‑211 (MQ=255)                                                 aTCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCCAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcag                                                                                                                                                                                    >  1:87582/1‑229 (MQ=255)                                                  tCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcag                                                                                                                                                                                    >  1:154701/1‑228 (MQ=255)                                                    tattatCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcagaac                                                                                                                                                                                 <  1:47873/251‑26 (MQ=255)                                                         tctctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggc                                                                                                                                                                            <  1:740715/251‑2 (MQ=255)                                                            ctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggca                                                                                                                                                                           >  1:91141/1‑247 (MQ=255)                                                            ctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCCATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggca                                                                                                                                                                           >  1:54289/1‑247 (MQ=255)                                                                       ccTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggcactgaaactgagaa                                                                                                                                                              >  1:167761/1‑236 (MQ=255)                                                                                                                                                         tAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTc                                                                       <  1:543537/251‑1 (MQ=255)                                                                                                                                                         tAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTc                                                                       <  1:2960/251‑1 (MQ=255)                                                                                                                                                                 aTTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTA‑GATCTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCa                                                                 >  1:454188/1‑249 (MQ=255)                                                                                                                                                                 aTTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTA‑GATCTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCa                                                                 >  1:448427/1‑249 (MQ=255)                                                                                                                                                                         cggAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATagag                                                       <  1:885580/250‑1 (MQ=255)                                                                                                                                                                           gagaCTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTGTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAgaga                                                      <  1:453155/250‑1 (MQ=255)                                                                                                                                                                             gactatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTc                                                                       >  1:279359/16‑231 (MQ=255)                                                                                                                                                                               ctatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGAt                                                           <  1:459579/228‑1 (MQ=255)                                                                                                                                                                               ctatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATcga                                                 >  1:894938/14‑249 (MQ=255)                                                                                                                                                                               ctatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATcga                                                 >  1:479787/14‑249 (MQ=255)                                                                                                                                                                               ctatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATcga                                                 >  1:62278/14‑249 (MQ=255)                                                                                                                                                                               ctatgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATcga                                                 >  1:773401/14‑249 (MQ=255)                                                                                                                                                                                 atgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAag                                               <  1:551970/240‑1 (MQ=255)                                                                                                                                                                                 atgtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAag                                               >  1:780671/12‑251 (MQ=255)                                                                                                                                                                                   gtatctgtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAagtt                                             >  1:606079/10‑251 (MQ=255)                                                                                                                                                                                         gtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAagttagtcat                                       >  1:877339/4‑248 (MQ=255)                                                                                                                                                                                         gtttataGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAagttagtcat                                       >  1:464845/4‑248 (MQ=255)                                                                                                                                                                                                   agcaaaccgCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAgaga                                                      >  1:445055/9‑226 (MQ=255)                                                                                                                                                                                                         ccgCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAAGTTAGTCATATTACCTGTCGAAaat                       <  1:725284/249‑1 (MQ=255)                                                                                                                                                                                                                     cccgcAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAAAAAGCTAAAAAATTCTATTc                                                                  >  1:63357/3‑196 (MQ=255)                                                                                                                                                                                                                         caatacTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAAGTTAGTCATATTACCTGTCGAAAATAATACTCAAGAAGTTg       >  1:410931/5‑251 (MQ=255)                                                                                                                                                                                                                           atacTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAAt                                                    <  1:857967/202‑1 (MQ=255)                                                                                                                                                                                                                             acTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACGAGCTAAAAAATTCTATTCACTGGATagag                                                       >  1:454210/1‑199 (MQ=255)                                                                                                                                                                                                                              cTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAGAGAATCGAAGTTAGTCATATTGCCTGTCGAAAATAATACTCAAGAAGTTGATTCa  >  1:108817/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      CTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAACGATCCTAGATTCTTTACATCCAGCCTAGATCCGAATCAAGAAGTTGCATATCGCATCTATGTTAAATCTGGTGATGATTACATTCTAAATACTATTTTAAGTGGAGACTATGTTGCTATCTATAGCCAACAAATTGCTTCAGATTCGCGCAACACTTATGATCGTAAAGATTTAGTTAAAATTTTAGGTAAAGTATTGGTTTTACAAGCAAGAAACTTTACTCAATCAACAGAACAAGGTGCTCCTGAAGCTGAAAAGGCAGCA‑GA‑CGGGATTCCAAACAATCAAGAATATGCTGGTTATGTAGCACTAAAAATAACTGCTGAACAAGCAAAAAAATTCTATTCACTGGATAGAGAATCAAAGTTAGTTATATTACCTGCCGAAAATAATACTCAAGAAGTTGATTCA  >  1/1479504‑1479978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑