Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,479,690	C→T	I167V (ATC→GTT)	unknown →	Flp pilus assembly protein RcpC/CpaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,479,690	0	C	T	100.0%	98.3 / NA	38	I167V (ATC→GTT)	unknown	Flp pilus assembly protein RcpC/CpaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

ACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAACGATCCTAGATTCTTTACATCCAGCCTAGATCCGAATCAAGAAGTTGCATATCGCATCTATGTTAAATCTGGTGATGATTACATTCTAAATACTATTTTAAGTGGAGACTATGTTGCTATCTATAGCCAACAAATTGCTTCAGATTCGCGCAACACTTATGATCGTAAAGATTTAGTTAAAATTTTAGGTAAAGTATTGGTTTTACAAGCAAGAAACTTTACTCAATCAACAGAACAAGGTGCTCCTGAAGCTGAAAAGGCAGCA‑GA‑CGGGATTCCAAACAATCAAGAATATGCTGGTTATGTAGCACTAAAAATAACTGCTGAACAAGCAAAAAAATTCTATTCACTGGATAGAGA  >  1/1479458‑1479926                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               aCCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCaaaccg                                                                                                                                                                                                                                >  1:144065/1‑246 (MQ=255)     aaGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAg                                                                                                                                                                                                                          >  1:125742/1‑251 (MQ=255)                  aCTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCaatac                                                                                                                                                                                                             <  1:769804/251‑4 (MQ=255)                       aaaTGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATg                                                                                                                                                                                                        <  1:98335/251‑1 (MQ=255)                         aTGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCaatac                                                                                                                                                                                                             >  1:187440/1‑241 (MQ=255)                         aTGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGAt                                                                                                                                                                                                      <  1:10668/251‑1 (MQ=255)                         aTGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGAt                                                                                                                                                                                                      <  1:626647/251‑1 (MQ=255)                            gTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGt                                                                                                                                                                                                   >  1:218883/1‑251 (MQ=255)                                   gggATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATg                                                                                                                                                                                                        <  1:209340/239‑1 (MQ=255)                                    ggATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGa                                                                                                                                                                                              <  1:667185/248‑1 (MQ=255)                                      aTTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAg                                                                                                                                                                                         >  1:553043/1‑251 (MQ=255)                                            aGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGtt                                                                                                                                                                                       <  1:8770/247‑1 (MQ=255)                                              gCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTaaagtt                                                                                                                                                                                 >  1:283197/1‑248 (MQ=255)                                               cTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGttt                                                                                                                                                                                >  1:263498/1‑251 (MQ=255)                                                 aTTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTa                                                                                                                                                                              >  1:105407/1‑251 (MQ=255)                                                  tttAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAg                                                                                                                                                                             <  1:35820/251‑1 (MQ=255)                                                          cAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggc                                                                                                                                                                      <  1:366099/250‑4 (MQ=255)                                                                aTGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggcagtggtt                                                                                                                                                               >  1:605186/1‑241 (MQ=255)                                                                           ggTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacg                                                                                                                                                    >  1:412097/1‑249 (MQ=255)                                                                           ggTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaag                                                                                                                                                        >  1:141345/1‑247 (MQ=255)                                                                            gTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacgg                                                                                                                                                   >  1:682175/1‑248 (MQ=255)                                                                               cTTTTCTATCCAACAAATCTATTATCTTTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGAt                                                                                                                                                                                                      <  1:432130/197‑1 (MQ=255)                                                                                 tttCTATCCAACAAATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattt                                                                                                                                              >  1:564018/1‑243 (MQ=255)                                                                                         caacaaATCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCTTATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggc                                                                                                                                                                      <  1:841199/219‑4 (MQ=255)                                                                                              aaTCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTaaggcagtggtt                                                                                                                                                               >  1:716179/1‑211 (MQ=255)                                                                                               aTCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCCAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcag                                                                                                                                >  1:87582/1‑229 (MQ=255)                                                                                                tCTATTATCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcag                                                                                                                                >  1:154701/1‑228 (MQ=255)                                                                                                  tattatCTCTCCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTAcaagcacggaattttgctcaagtagcagaac                                                                                                                             <  1:47873/251‑26 (MQ=255)                                                                                                       tctctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggc                                                                                                                        <  1:740715/251‑2 (MQ=255)                                                                                                          ctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggca                                                                                                                       >  1:91141/1‑247 (MQ=255)                                                                                                          ctcCGAATGATCCTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCCATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggca                                                                                                                       >  1:54289/1‑247 (MQ=255)                                                                                                                     ccTAGATTCTTTACTTCCAGCTTGAATCCAAGTCAAGAAGTCGCATATCGCATCTATGTTAGATCTGGCGATGATTACATTCTAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAggcactgaaactgagaa                                                                                                          >  1:167761/1‑236 (MQ=255)                                                                                                                                                                                                       tAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTc                   <  1:543537/251‑1 (MQ=255)                                                                                                                                                                                                       tAAATACTATTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTc                   <  1:2960/251‑1 (MQ=255)                                                                                                                                                                                                               aTTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTA‑GATCTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCa             >  1:454188/1‑249 (MQ=255)                                                                                                                                                                                                               aTTTTAAGCGGAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTA‑GATCTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCa             >  1:448427/1‑249 (MQ=255)                                                                                                                                                                                                                       cggAGACTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTTTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATagag   <  1:885580/250‑1 (MQ=255)                                                                                                                                                                                                                         gagaCTATGTATCTGTTTATAGCCAGCAAACCGCTTCAGATTCCCGCAATACTTATGATCGTAAAGATTTAGTTAAAGTTGTAGGTAAGGCAGTGGTTCTACAAGCACGGAATTTTGCTCAAGTAGCAGAACAAG‑‑GC‑ACTGAAACTGAGAAAGCGTTAGGT‑CTGG‑CTCCAAATAGTGAAGTATATGCCGGTTATGTAGCATTAAAAATAACTGCTGAACAAGCTAAAAAATTCTATTCACTGGATAgaga  <  1:453155/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               ACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATCTCTCCGAACGATCCTAGATTCTTTACATCCAGCCTAGATCCGAATCAAGAAGTTGCATATCGCATCTATGTTAAATCTGGTGATGATTACATTCTAAATACTATTTTAAGTGGAGACTATGTTGCTATCTATAGCCAACAAATTGCTTCAGATTCGCGCAACACTTATGATCGTAAAGATTTAGTTAAAATTTTAGGTAAAGTATTGGTTTTACAAGCAAGAAACTTTACTCAATCAACAGAACAAGGTGCTCCTGAAGCTGAAAAGGCAGCA‑GA‑CGGGATTCCAAACAATCAAGAATATGCTGGTTATGTAGCACTAAAAATAACTGCTGAACAAGCAAAAAAATTCTATTCACTGGATAGAGA  >  1/1479458‑1479926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑