Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,479,333	A→G	K48K (AAA→AAG)	unknown →	Flp pilus assembly protein RcpC/CpaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,479,333	0	A	G	100.0%	158.3 / NA	59	K48K (AAA→AAG)	unknown	Flp pilus assembly protein RcpC/CpaB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/23);  total (36/23)

GTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAGACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAACATTTGACAATACTGAAAATCAACAAACAATAATAAATAATCAGCAGGTTCCGGAAGCTGAGAAAAAACCGGAAAAGCTAATTTTAACTGCCGAGTTAAAGAATGACGTAAGCAAAGGTCATTTATTACAGGCAGATGATTATATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTAGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATC  >  1/1479091‑1479561                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     gcaataagccttggctttatgataaacatggctctatcaaattaattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGaaaa                                                                                                                                                                                                                              >  1:807160‑M2/204‑251 (MQ=255)                 ttatgataaacatggctctatcaaattaattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGc                                                                                                                                                                                                               >  1:31455‑M2/188‑250 (MQ=255)                          acatggctctatcaaattaattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAg                                                                                                                                                                                                     >  1:369374‑M2/179‑251 (MQ=255)                               gctctatcaaattaattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGa                                                                                                                                                                                                >  1:146274‑M2/174‑251 (MQ=255)                                        aattaattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCaa                                                                                                                                                                                       >  1:750939‑M2/165‑251 (MQ=255)                                            aattttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGt                                                                                                                                                                                   >  1:545283‑M2/161‑251 (MQ=255)                                             attttgattgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGt                                                                                                                                                                                   >  1:166806‑M2/160‑250 (MQ=255)                                                   attgagcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcctacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATtta                                                                                                                                                                             <  1:436300‑M2/97‑1 (MQ=255)                                                       agcggaaactttactttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTAc                                                                                                                                                                         <  1:707386‑M2/101‑1 (MQ=255)                                                                      ttgttaaaatttaattaagggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATgattacat                                                                                                                                                         <  1:12302‑M2/117‑4 (MQ=255)                                                                                         ggtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGaaa                                                                                                                                                                                                                               >  1:465431‑M2/116‑162 (MQ=255)                                                                                          gtaaaaaatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTc                                                                                                                                      >  1:623529‑M2/115‑250 (MQ=255)                                                                                                aatgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTGCAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAg                                                                                                                               <  1:232269‑M2/143‑1 (MQ=255)                                                                                                 atgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGa                                                                                                                              >  1:497831‑M2/108‑251 (MQ=255)                                                                                                  tgaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAGAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGAc                                                                                                                             <  1:212368‑M2/145‑1 (MQ=255)                                                                                                   gaattacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTAc                                                                                                                                                                         >  1:578539‑M2/106‑206 (MQ=255)                                                                                                     attacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAAt                                                                                                                                                                                                  <  1:378179‑M2/76‑1 (MQ=255)                                                                                                      ttacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAgatg                                                                                                                                                                >  1:565284‑M2/103‑212 (MQ=255)                                                                                                      ttacagaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATgattacat                                                                                                                                                         <  1:789563‑M2/117‑4 (MQ=255)                                                                                                           gaacgctcttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCa                                                                                                                       >  1:399151‑M2/98‑248 (MQ=255)                                                                                                                   ttgatcatttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACaa                                                                                                            <  1:774508‑M2/162‑1 (MQ=255)                                                                                                                          tttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCaa                                                                                                                                                                                       <  1:460218‑M2/87‑1 (MQ=255)                                                                                                                          tttcagtgcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTa                                                                                                     >  1:10872‑M2/83‑251 (MQ=255)                                                                                                                                 gcttacgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAg                                                                                                                                                                                                     <  1:380861‑M2/73‑1 (MQ=255)                                                                                                                                      cgctagcggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACg                                                                                         >  1:178130‑M2/71‑251 (MQ=255)                                                                                                                                            cggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATttat                                                                                                                                                                            <  1:186333‑M2/98‑1 (MQ=255)                                                                                                                                             ggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGc                                                                                             >  1:674694‑M2/64‑240 (MQ=255)                                                                                                                                             ggttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTaaaa                                                                                  >  1:235054‑M2/64‑251 (MQ=255)                                                                                                                                              gttggcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAagag                                                                                                 <  1:237051‑M2/173‑1 (MQ=255)                                                                                                                                                  gcattggtggcattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGc                                                                                                                        >  1:471454‑M2/59‑208 (MQ=255)                                                                                                                                                            cattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATtta                                                                                                                                                                             <  1:883051‑M2/97‑1 (MQ=255)                                                                                                                                                            cattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGc                                                                                                                                                                     <  1:278403‑M2/105‑1 (MQ=255)                                                                                                                                                            cattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCAc                                                                                                                      >  1:562842‑M2/49‑200 (MQ=255)                                                                                                                                                            cattatgtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGAt                                                                   >  1:481173‑M2/49‑251 (MQ=255)                                                                                                                                                                  gtttatgccagcatttgacaatactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAg                                                             >  1:150762‑M2/43‑251 (MQ=255)                                                                                                                                                                                      atactgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAAt                                         >  1:873148‑M2/23‑251 (MQ=255)                                                                                                                                                                                         ctgaaaatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAgg                                                                      >  1:37633‑M2/20‑219 (MQ=255)                                                                                                                                                                                              aatcaacaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGc                                 <  1:376894‑M2/237‑1 (MQ=255)                                                                                                                                                                                                   acaaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACCATGACCTAAGAGAGGCTACGAc                                                                                       >  1:433360‑M2/10‑192 (MQ=255)                                                                                                                                                                                                    caaacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTc                           <  1:828673‑M2/243‑1 (MQ=255)                                                                                                                                                                                                      aacaatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGAc                                                                                       <  1:502241‑M2/183‑1 (MQ=255)                                                                                                                                                                                                        caataataatAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTg                                                                                                                                  >  1:41512/5‑144 (MQ=255)                                                                                                                                                                                                        caatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTAc                                                                >  1:504269‑M2/5‑210 (MQ=255)                                                                                                                                                                                                        caatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGc                                                           >  1:641245‑M2/5‑215 (MQ=255)                                                                                                                                                                                                        caatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCtttt                       >  1:133137‑M2/5‑251 (MQ=255)                                                                                                                                                                                                        caatAATAATAAATAATCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCtttt                       >  1:732558‑M2/5‑251 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAagag                                                                                                 <  1:286945/168‑1 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAagag                                                                                                 >  1:159570/1‑168 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTaa                                                    >  1:106041/1‑213 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGc                                 >  1:242615/1‑232 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCcaa               >  1:90435/1‑250 (MQ=255)                                                                                                                                                                                                                 taaataaTCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCcaa               >  1:100097/1‑250 (MQ=255)                                                                                                                                                                                                                     taatCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAagag                                                                                                 <  1:232937/164‑1 (MQ=255)                                                                                                                                                                                                                     taatCAGTCGGTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAAt          >  1:485190/1‑251 (MQ=255)                                                                                                                                                                                                                      aatcagtcggTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAAt          >  1:11954/9‑250 (MQ=255)                                                                                                                                                                                                                             cggTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTAtc  <  1:665572/250‑1 (MQ=255)                                                                                                                                                                                                                             cggTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTAtc  <  1:279032/250‑1 (MQ=255)                                                                                                                                                                                                                               gTTCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTAtc  <  1:105153/249‑1 (MQ=255)                                                                                                                                                                                                                                 tCCGGAAACTGAGAAAAAGCCGGAAAAGCTAATTTTAACTGCCGAATTAAAGAATGACGTGAGCAAAGGTCATTTATTACAGGCAGATGATTACATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTGGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGc                                 <  1:347805/216‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     GTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAGACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAACATTTGACAATACTGAAAATCAACAAACAATAATAAATAATCAGCAGGTTCCGGAAGCTGAGAAAAAACCGGAAAAGCTAATTTTAACTGCCGAGTTAAAGAATGACGTAAGCAAAGGTCATTTATTACAGGCAGATGATTATATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTAGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCTATTTAATGGCAGAAAATGTCAGAGCTGGTTCTTTTCTATCCAACAAATCTATTATC  >  1/1479091‑1479561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑