Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,479,265	A→G	T26A (ACA→GCA)	unknown →	Flp pilus assembly protein RcpC/CpaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,479,265	0	A	G	100.0%	152.8 / NA	54	T26A (ACA→GCA)	unknown	Flp pilus assembly protein RcpC/CpaB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/23);  total (31/23)

TGGGACTAATTATTATCGGTTGGTTATTTTTCCGTGAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAGACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAACATTTGACAATACTGAAAATCAACAAACAATAATAAATAATCAGCAGGTTCCGGAAGCTGAGAAAAAACCGGAAAAGCTAATTTTAACTGCCGAGTTAAAGAATGACGTAAGCAAAGGTCATTTATTACAGGCAGATGATTATATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTAGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCT  >  1/1479023‑1479505                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 tGGGACTAATTATTATCGGTTGGTTATTTTTCCGTAAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCCTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGAc                                                                                                                                                                                                                                          <  1:433269/251‑1 (MQ=255)    gACTAATTATTATCGGTTGGTTATTTTTCCGTAAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAAt                                                                                                                                                                                                                                       >  1:221779/1‑251 (MQ=255)      cTAATTATTATCGGTTGGTTATTTTTCCGTAAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATAc                                                                                                                                                                                                                                     <  1:583586/251‑1 (MQ=255)                        ttATTTTTCCGTAAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaat                                                                                                                                                                                                                   >  1:109763‑M1/1‑248 (MQ=255)                           tttttCCGTAAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataat                                                                                                                                                                                                                <  1:665856‑M1/251‑7 (MQ=255)                                               aGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggtt                                                                                                                                                                                             >  1:330225‑M1/1‑225 (MQ=255)                                               aGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggtt                                                                                                                                                                                             >  1:248567‑M1/1‑225 (MQ=255)                                                       gATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaac                                                                                                                                                                                     <  1:147695‑M1/250‑34 (MQ=255)                                                           gCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGAc                                                                                                                                                                                                                                          >  1:260366/1‑193 (MQ=255)                                                           gCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCaacaaac                                                                                                                                                                                                                      <  1:281142/213‑1 (MQ=255)                                                           gCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgaga                                                                                                                                                                                >  1:896131‑M1/1‑213 (MQ=255)                                                           gCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaac                                                                                                                                                                                     <  1:48559‑M1/246‑34 (MQ=255)                                                              ttATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtc                                                                                                                                                                                                 <  1:524854‑M1/231‑22 (MQ=255)                                                                        gCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaa                                                                                                                                                                   >  1:807160‑M1/1‑200 (MQ=255)                                                                                        ttATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgc                                                                                                                                                    >  1:31455‑M1/1‑184 (MQ=255)                                                                                          aTGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGaaa                                                                                                                                                                                                                                >  1:900391/1‑172 (MQ=255)                                                                                          aTGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaa                                                                                                                                                                                       >  1:564812‑M1/1‑182 (MQ=255)                                                                                                 aCATGGCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaag                                                                                                                                          >  1:369374‑M1/1‑175 (MQ=255)                                                                                                      gCTCTATCAAATTAATTTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatga                                                                                                                                     >  1:146274‑M1/1‑170 (MQ=255)                                                                                                               aattaattTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaa                                                                                                                            >  1:750939‑M1/1‑161 (MQ=255)                                                                                                                   aattTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggt                                                                                                                        >  1:545283‑M1/1‑157 (MQ=255)                                                                                                                    attTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggt                                                                                                                        >  1:166806‑M1/1‑156 (MQ=255)                                                                                                                    attTTGATTGAGCGGAAACTTTACTTTGTTAAAATTTAATT‑AGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaa                                                                                                                                                                                       >  1:651670‑M1/1‑155 (MQ=255)                                                                                                                          attgaGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCCTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcattta                                                                                                                  <  1:436300‑M1/250‑101 (MQ=255)                                                                                                                              aGCGGAAACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattac                                                                                                              <  1:707386‑M1/250‑105 (MQ=255)                                                                                                                                             ttGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacat                                                                                              <  1:12302‑M1/251‑121 (MQ=255)                                                                                                                                               gTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCaacaaac                                                                                                                                                                                                                      >  1:746964/1‑129 (MQ=255)                                                                                                                                                                ggTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaa                                                                                                                                                                    >  1:465431‑M1/1‑112 (MQ=255)                                                                                                                                                                 gTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtc                                                                           >  1:623529‑M1/1‑111 (MQ=255)                                                                                                                                                                       aaTGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattgcaggcagatgattacattttaaatgagatttctgtccctgaag                                                                    <  1:232269‑M1/251‑147 (MQ=255)                                                                                                                                                                        aTGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaaga                                                                   >  1:497831‑M1/1‑104 (MQ=255)                                                                                                                                                                         tGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaagagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagac                                                                  <  1:212368‑M1/251‑149 (MQ=255)                                                                                                                                                                          gAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattac                                                                                                              >  1:578539‑M1/1‑102 (MQ=255)                                                                                                                                                                            aTTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaat                                                                                                                                       <  1:378179‑M1/179‑80 (MQ=255)                                                                                                                                                                             ttACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacat                                                                                              <  1:789563‑M1/219‑121 (MQ=255)                                                                                                                                                                             ttACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatg                                                                                                     >  1:565284‑M1/1‑99 (MQ=255)                                                                                                                                                                                  gAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgca                                                            >  1:399151‑M1/1‑94 (MQ=255)                                                                                                                                                                                       ctctTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatc                                                                                                                                                                                                     <  1:595066‑M1/106‑18 (MQ=255)                                                                                                                                                                                          ttGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaa                                                 <  1:774508‑M1/251‑166 (MQ=255)                                                                                                                                                                                                 tttCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgaccta                                          >  1:10872‑M1/1‑79 (MQ=255)                                                                                                                                                                                                 tttCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaa                                                                                                                            <  1:460218‑M1/169‑91 (MQ=255)                                                                                                                                                                                                        gCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaag                                                                                                                                          <  1:380861‑M1/148‑77 (MQ=255)                                                                                                                                                                                                             cGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagagaggctacg                              >  1:178130‑M1/1‑67 (MQ=255)                                                                                                                                                                                                                   cGGTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttat                                                                                                                 <  1:186333‑M1/162‑102 (MQ=255)                                                                                                                                                                                                                    ggTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagagaggctacgactaaaa                       >  1:235054‑M1/1‑60 (MQ=255)                                                                                                                                                                                                                    ggTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagagaggc                                  >  1:674694‑M1/1‑60 (MQ=255)                                                                                                                                                                                                                     gTTGGCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagag                                      <  1:237051‑M1/235‑177 (MQ=255)                                                                                                                                                                                                                         gCATTGGTGGCATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgc                                                             >  1:471454‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                                   cATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagagaggctacgactaaaaatggtattcagggat        >  1:481173‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                                   cATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcac                                                           >  1:562842‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                                   cATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggc                                                                                                          <  1:278403‑M1/153‑109 (MQ=255)                                                                                                                                                                                                                                   cATTATGTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcattta                                                                                                                  <  1:883051‑M1/145‑101 (MQ=255)                                                                                                                                                                                                                                         gTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagtcggttccggaaactgagaaaaagccggaaaagctaattttaactgccgaattaaagaatgacgtgagcaaaggtcatttattacaggcagatgattacattttaaatgagatttctgtccctgaagacagtgcactggtcgacaatgacctaagagaggctacgactaaaaatggtattcagggattacaag  >  1:150762‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                                         gTTTATGCCAGCATTTGACAATACTGAAAATCAACAAACaataataataaataatcagt                                                                                                                                                                                                  <  1:467667‑M1/59‑21 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 TGGGACTAATTATTATCGGTTGGTTATTTTTCCGTGAATCAATTAAAGAAAACGGATTGCCTTATGGCGTAGCAATAAGCCTTGGCTTTATGATAAACATGGCTCTATCAAATTAATTTTGATTGAGCGGAGACTTTACTTTGTTAAAATTTAATTAAGGGTAAAAAATGAATTACAGAACGCTCTTGATCATTTCAGTGCTTACGCTAGCGGTTGGCATTGGTGGCATTATGTTTATGCCAACATTTGACAATACTGAAAATCAACAAACAATAATAAATAATCAGCAGGTTCCGGAAGCTGAGAAAAAACCGGAAAAGCTAATTTTAACTGCCGAGTTAAAGAATGACGTAAGCAAAGGTCATTTATTACAGGCAGATGATTATATTTTAAATGAGATTTCTGTCCCTGAAGACAGTGCACTAGTCGACAATGACCTAAGAGAGGCTACGACTAAAAATGGTATTCAGGGATTACAAGGCT  >  1/1479023‑1479505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑