Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	225,350	G→A	intergenic (‑26/+42)	unknown ← / ← unknown	hypothetical protein/Superoxide dismutase [Cu‑Zn] precursor (EC 1.15.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	225,350	0	G	A	100.0%	182.4 / NA	69	intergenic (‑26/+42)	unknown/unknown	hypothetical protein/Superoxide dismutase [Cu‑Zn] precursor (EC 1.15.1.1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/38);  total (31/38)

GATTTTACGCCATTAAGCCCTCTTTCACGAAATGCTTTTAGCCAATAAATGACGAGCGCGTGGGAGATTTTATGAAGTTTCGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCGGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATGGGCGT  >  1/225167‑225594                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATAcg                                                                                                                                                                                  <  1:624914/250‑1 (MQ=255)  aTTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATAcgcg                                                                                                                                                                                >  1:661990/1‑251 (MQ=11)   ttttACGCCATTAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATAcg                                                                                                                                                                                  <  1:226559/248‑1 (MQ=255)            aTTAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATAcgc                                                                                                                                                                                 >  1:303672/1‑240 (MQ=11)            aTTAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGAccgccgc                                                                                                                                                                       >  1:675978/1‑250 (MQ=11)                                                     cttgagaacgagaaatctgaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGccac                                                                                                                                >  1:105708/20‑250 (MQ=18)                                                             cgagaaatctgaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGa                                                                                                                       >  1:443709/12‑251 (MQ=21)                                                               agaaatctgaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGAt                                                                                                                      <  1:846594/241‑1 (MQ=21)                                                                     ctgaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAgg                                                                                                               >  1:134948/4‑251 (MQ=255)                                                                      tgaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAggg                                                                                                              <  1:555863/249‑1 (MQ=255)                                                                       gaTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGcc                                                                                                                                  >  1:737475/2‑230 (MQ=255)                                                                        aTGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTGGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAg                                                                                                            <  1:514412/251‑1 (MQ=255)                                                                            agttcagCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGAccgccgccg                                                                                                                                                                     >  1:518583/7‑190 (MQ=255)                                                                             gttcagCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGAc                                                                                                        >  1:475827/6‑250 (MQ=255)                                                                              ttcagCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACcg                                                                                                      <  1:631828/247‑1 (MQ=255)                                                                              ttcagCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACcg                                                                                                      <  1:635018/247‑1 (MQ=255)                                                                                     aCCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAg                                                                                                                <  1:592600/234‑1 (MQ=255)                                                                                     aCCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCAGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAg                                                                                                                >  1:434363/1‑234 (MQ=255)                                                                                      ccTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTa                                                                                                                                       <  1:156129/210‑1 (MQ=255)                                                                                         cACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATc                                                                                                                     <  1:591187/225‑1 (MQ=255)                                                                                              aTACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAg                                                                                       <  1:11409/250‑1 (MQ=255)                                                                                              aTACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAg                                                                                       <  1:93824/250‑1 (MQ=255)                                                                                              aTACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAg                                                                                       <  1:237662/250‑1 (MQ=255)                                                                                               tACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCAc                                                                                                 >  1:504748/1‑239 (MQ=255)                                                                                               tACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAgtg                                                                                     <  1:790635/251‑1 (MQ=255)                                                                                                aCCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTAttt                                                                                                                                                                                                       <  1:666057/136‑1 (MQ=255)                                                                                                      aTCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATg                                                                              <  1:732022/251‑1 (MQ=255)                                                                                                        ccTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCg                                                                            <  1:735912/251‑1 (MQ=255)                                                                                                                     cTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTac                                                                                                                                                                                                 <  1:524268/121‑1 (MQ=255)                                                                                                                     cTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGCCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAAt                                                                               <  1:645318/235‑1 (MQ=255)                                                                                                                       gTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGcc                                                                                                                                  <  1:792451/182‑1 (MQ=255)                                                                                                                       gTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCgg                                                             >  1:493246/1‑251 (MQ=255)                                                                                                                                       aCGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCcg                                             <  1:337967/251‑1 (MQ=255)                                                                                                                                          aaaTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCgcgc                                                                                                   <  1:875171/194‑1 (MQ=255)                                                                                                                                          aaaTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCcgtcg                                          >  1:230440/1‑251 (MQ=255)                                                                                                                                            aTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTacac                                                                                                                                                                                               <  1:294438/100‑1 (MQ=255)                                                                                                                                            aTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCgtg                                        >  1:21222/1‑251 (MQ=255)                                                                                                                                              tGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATAcg                                                                                                                                                                                  <  1:855217/111‑1 (MQ=255)                                                                                                                                               gATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTaa                                     >  1:778704/1‑251 (MQ=255)                                                                                                                                                 tAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATa                                   <  1:606374/251‑1 (MQ=255)                                                                                                                                                  aaaagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATAc                                  <  1:208336/248‑1 (MQ=255)                                                                                                                                                  aaGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATAc                                  <  1:739191/251‑1 (MQ=255)                                                                                                                                                  aaGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATAc                                  <  1:795897/251‑1 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGc                                                                                                                                                          >  1:282042/1‑129 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAg                                                                                                                >  1:93541/1‑171 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAgtg                                                                                     <  1:402779/198‑1 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTaa                                     <  1:116140/246‑1 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACgg                                >  1:643680/1‑251 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACgg                                >  1:705054/1‑251 (MQ=255)                                                                                                                                                    gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGCGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGCGCCGTCGTGTAATACgg                                >  1:672014/1‑251 (MQ=255)                                                                                                                                                     agTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGc                                                                                                                                   >  1:713740/1‑151 (MQ=255)                                                                                                                                                     agTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAg                                                                                                                <  1:548276/170‑1 (MQ=255)                                                                                                                                                      gTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTa                                                                                                                                                                                                  >  1:296328/1‑87 (MQ=255)                                                                                                                                                          gACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGAt                                                                                                                      >  1:272129/1‑159 (MQ=255)                                                                                                                                                          gACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAg                                                                                       >  1:779182/1‑190 (MQ=255)                                                                                                                                                          gACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATg                                                                              >  1:55891/1‑199 (MQ=255)                                                                                                                                                          gACATAATCTGCACCTCAAATTAGGCGTCCAGGTTTTGGGGCGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATg                          >  1:851925/1‑251 (MQ=255)                                                                                                                                                           aCATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGc                         <  1:546932/251‑1 (MQ=255)                                                                                                                                                              tAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCgg                      >  1:506184/1‑251 (MQ=255)                                                                                                                                                                aTCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAg                                                                                                                >  1:667060/1‑159 (MQ=255)                                                                                                                                                                       ccTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTacac                                                                                                                                                                                               <  1:340852/73‑1 (MQ=255)                                                                                                                                                                       ccTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTCAATGCCGGTAAATCAc              >  1:757246/1‑250 (MQ=255)                                                                                                                                                                          cAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACcgcg                                                                                                    >  1:262472/1‑161 (MQ=255)                                                                                                                                                                          cAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTa                                                                                         <  1:510069/172‑1 (MQ=255)                                                                                                                                                                          cAAATTAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGc                         <  1:263269/236‑1 (MQ=255)                                                                                                                                                                              ttAGGTGTCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATgg      <  1:143239/251‑1 (MQ=255)                                                                                                                                                                                  gtgtCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATGGGCg   <  1:288113/250‑1 (MQ=255)                                                                                                                                                                                  gtgtCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATGGGCGt  >  1:216579/1‑251 (MQ=255)                                                                                                                                                                                    gtCCAGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATGGGCg   <  1:543662/248‑1 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GATTTTACGCCATTAAGCCCTCTTTCACGAAATGCTTTTAGCCAATAAATGACGAGCGCGTGGGAGATTTTATGAAGTTTCGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAGTTTTTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCGGGTTTTGGGGTGCAGATCACTTTTTTGTTTCGGAAAGCGGATTATTTAATTACACCACACGCCATACGCGGACCGCCGCCGCCAAGTGGGGCCGGGTGATCGGAGTGGTTATCGCCACCAGCGTGGATCATGAGGGAGTGACCGCGCACGTCGTCTAAGTGTTTAATGCGTGGGGCTAAAACCGGGTTAGCGGCTGTGCCGTCGTGTAATACGGTTAATGCCGGTAAATCACCTAAATGGGCGT  >  1/225167‑225594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑