Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,478,605	A→G	E48G (GAG→GGG)	unknown →	Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,478,605	0	A	G	100.0%	115.3 / NA	43	E48G (GAG→GGG)	unknown	Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/17);  total (26/17)

GAGCTATTCTACACTCTATATTAGAATTAATTATGATGGATTTACTGGATTACTTTTATCGTCAAGTAGTCTTTTTTTCGTGTAAGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGCTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGAGGATAACAGCTTTATTAAGGCGTTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCTCTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGATAAAAAAATGAACTGGCTTATTAATACTTTAGTAGTCATAACGATCTTACTGCTGACTACTTTATCTGTAACTGACATTCGTTCTCGGATTATTAGTAACCGAGTCGTTTTGTTTCTGTTATTCGTCATTATTCCGTTCAGTTTATT  >  1/1478431‑1478849                                                                                                                                                                               |                                                                                                                                                                                                                                                     gggctattctttgcttcattttgaaattaattATGATGGATTTACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAg                                                                                                                                                                           >  1:880613/25‑250 (MQ=255)  ggctattctttgcttcattttgaaattaattATGATGGATTTACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGc                                                                                                                                                                          <  1:62568/227‑1 (MQ=255)    ctattctttgcttcattttgaaattaattATGATGGATTTACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCaaa                                                                                                                                                                       <  1:392439/230‑1 (MQ=255)                      tgaaattaattATGATGGATTTACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGa                                                                                                                                                     <  1:159307/248‑1 (MQ=255)                                       gATTTACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTgg                                                                                                                                     >  1:886480/1‑249 (MQ=255)                                         tttACTGGATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCtta                                                                                                                                 >  1:599507/1‑251 (MQ=255)                                               ggATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTaacg                                                                                                                           >  1:230394/1‑249 (MQ=255)                                               ggATTACTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGAATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTaacg                                                                                                                           >  1:452470/1‑249 (MQ=255)                                                     cTTTTATCATCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTttatta                                                                                                                                                                                                                                     <  1:214149/140‑1 (MQ=255)                                                          atcatCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTtagtaatc                                                                                                                <  1:835872/247‑4 (MQ=255)                                                           tcatCAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTtagt                                                                                                                    >  1:190674/4‑246 (MQ=255)                                                               cAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGATTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCttatta                                                                                                                              <  1:757379/232‑1 (MQ=255)                                                               cAAGTAGTCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCttatta                                                                                                                              >  1:263358/1‑232 (MQ=255)                                                                    agtCTTTTCTTCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTtagtaa                                                                                                                  >  1:149626/1‑238 (MQ=255)                                                                          ttcttCGTATAGGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGctctcgctgc                                                                                                <  1:2621/248‑7 (MQ=255)                                                                                gtataggTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGctgctggtta                                                                                          >  1:482912/7‑247 (MQ=255)                                                                                      gTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTAt                                                                                    >  1:366220/1‑251 (MQ=255)                                                                                        tttACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCt                                                                                  <  1:858182/251‑1 (MQ=255)                                                                                         ttACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCta                                                                                 <  1:291182/251‑2 (MQ=255)                                                                                                 aTCGACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGa                                                                          >  1:311721/1‑250 (MQ=255)                                                                                                    gACAAGGGATTACTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTtagtaa                                                                                                                  <  1:281763/207‑2 (MQ=255)                                                                                                                cTTCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCggctt                                                          >  1:674379/1‑248 (MQ=255)                                                                                                                  tCTGTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGACCTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGcttat                                                        >  1:430098/1‑251 (MQ=255)                                                                                                                     gTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGc                                                                                                                                                                                                                                            <  1:519820/69‑1 (MQ=255)                                                                                                                     gTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCttatta                                                                                                                              >  1:869767/1‑178 (MQ=255)                                                                                                                     gTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTtag                                                                                                                     >  1:583085/1‑187 (MQ=255)                                                                                                                     gTTGAGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAAc                                                                             <  1:462671/227‑1 (MQ=255)                                                                                                                         aGTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGt                                                                                                                                                                                                                       >  1:864982/1‑86 (MQ=255)                                                                                                                          gTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCta                                                                                 >  1:857845/1‑217 (MQ=255)                                                                                                                          gTATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAAc                                                 >  1:359386/1‑250 (MQ=255)                                                                                                                           tATGGGTTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAAc                                                 <  1:724868/249‑1 (MQ=255)                                                                                                                              gggttAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACttt                                                                                      <  1:663397/205‑1 (MQ=255)                                                                                                                               ggttAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTaacgc                                                                                                                          <  1:463532/169‑4 (MQ=255)                                                                                                                               ggttAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAAc                                                                             <  1:500053/214‑1 (MQ=255)                                                                                                                               ggttAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAACCGaatt                                           >  1:779923/4‑248 (MQ=255)                                                                                                                                gttAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAAc                                                                             >  1:152763/3‑216 (MQ=255)                                                                                                                                  tAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGc                                                                                                                                    >  1:825586/1‑159 (MQ=255)                                                                                                                                       gCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGctgctgg                                                                                             >  1:215862/1‑192 (MQ=255)                                                                                                                                                      gTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGt                                                                                                                                                                              >  1:698891/1‑98 (MQ=255)                                                                                                                                                      gTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAACCGAATTGTTTTGTTTCTGTTATTCGTCat                    >  1:85047/1‑251 (MQ=255)                                                                                                                                                      gTTTTTGTAGTCGCCGTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAACCGAATTGTTTTGTTTCTGTTATTCGTCat                    >  1:261506/1‑251 (MQ=255)                                                                                                                                                                     gTTTTAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAACCGAATTGTTTTGTTTCTGTTATTCGTCATTATTCCGTTCAGttt     >  1:725234/1‑251 (MQ=255)                                                                                                                                                                        ttAGTCGGGGATAACAGCTTTATTAAGGCAGTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCACTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGAT‑AAAAAATGAACTGGCTTATTAACGCTTTAGTAATCATAACGCTCTCGCTGCTGGTTACTTTATCTATAACTGACATTCGTTTTCGGCTTATTAGTAACCGAATTGTTTTGTTTCTGTTATTCGTCATTATTCCGTTCAGTttatt  <  1:843970/251‑1 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                                                                     GAGCTATTCTACACTCTATATTAGAATTAATTATGATGGATTTACTGGATTACTTTTATCGTCAAGTAGTCTTTTTTTCGTGTAAGTTTTACAGAAATCGACAAGGGATTACTTCTGTTGAGTATGGGCTAATTGCTGTGGCTATTGCTGTTTTTGTAGTCGCCGTTTTAGTCGAGGATAACAGCTTTATTAAGGCGTTGTCAGGTAAGTTCTCGGATTTAACGACCACTGTCTCTGACGCCATAGTTAGCAAAAGTAGCTAAGAAAAATGATAAAAAAATGAACTGGCTTATTAATACTTTAGTAGTCATAACGATCTTACTGCTGACTACTTTATCTGTAACTGACATTCGTTCTCGGATTATTAGTAACCGAGTCGTTTTGTTTCTGTTATTCGTCATTATTCCGTTCAGTTTATT  >  1/1478431‑1478849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑