Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,478,003	G→A	intergenic (‑587/‑162)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,478,003	0	G	A	100.0%	36.9 / NA	16	intergenic (‑587/‑162)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/6);  total (10/6)

TTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTAAATAAATGACATTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACCTTAATTACGAAAGCATACATCAAAGCAACCGAAGCAATCCGTTCTTTCAAAAAAAATCAAGCAGGTGTGA  >  1/1477814‑1478246                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ttCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatataccggt                                                                                                                                                                                           >  1:232544/1‑243 (MQ=255)          agaaaaaaGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac                                                                                                                                                                                               <  1:56866/234‑4 (MQ=255)                           cgcgATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCttatta                                                                                                                                                               <  1:354355/251‑1 (MQ=255)                                                                                     gTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATtta                                                                                                                                                                                                                                           >  1:146277/1‑119 (MQ=255)                                                                                                      tCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGAc                                                                                     <  1:225005/251‑1 (MQ=255)                                                                                                                 aGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac                                                                                                                                                                                               >  1:268495/1‑132 (MQ=255)                                                                                                                                  tACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACTTTAACTACTAAAGCa                                                         >  1:484344/1‑251 (MQ=255)                                                                                                                                    cAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAg                                                                                                                                                                      <  1:151415/140‑1 (MQ=255)                                                                                                                                          cccTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAg                                                                                       <  1:497732/213‑1 (MQ=255)                                                                                                                                               aaCATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGAc                                                                                     >  1:199993/1‑210 (MQ=255)                                                                                                                                                                                caATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac                                                                                                                                                                                               >  1:900580/1‑69 (MQ=255)                                                                                                                                                                                       tacatatacataGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATAc                                                                         >  1:382401/1‑182 (MQ=255)                                                                                                                                                                                         cataTACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCcaca                                                                                                                                                                        >  1:744965/1‑85 (MQ=255)                                                                                                                                                                                         cataTACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACac                                                                                                                                                   <  1:693226/106‑2 (MQ=255)                                                                                                                                                                                         cataTACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGAc                                                                                     >  1:566836/1‑168 (MQ=255)                                                                                                                                                                                         cataTACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACTTTAACTACTAAAGCATACATCAAAGCAGCTGAAGCAATCCGTTCTTTCAGAGAAAATCAAGCAGGTGTGa  >  1:110562/1‑251 (MQ=255)                                                                                                                                                                                           tataCATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACac                                                                                                                                                   >  1:666090/1‑103 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                                     TTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTAAATAAATGACATTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACCTTAATTACGAAAGCATACATCAAAGCAACCGAAGCAATCCGTTCTTTCAAAAAAAATCAAGCAGGTGTGA  >  1/1477814‑1478246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑