Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,810	A→G	intergenic (‑394/‑355)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,810	0	A	G	100.0%	29.7 / NA	12	intergenic (‑394/‑355)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

CAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATAC  >  1/1477620‑1478002                                                                                                                                                                                                |                                                                                                                                                                                                   cAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAg                                                                                                                                        <  1:563103/251‑1 (MQ=255)          aaGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGtg                                                                                                                                      >  1:578954/1‑243 (MQ=255)                        tgcttgttCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCcacaca                                                                                                                                                >  1:813579/4‑220 (MQ=255)                              ttCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTaaa                                                                                                                                                        >  1:702739/1‑206 (MQ=255)                                                                 gCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAAt                                                                        <  1:276276/250‑1 (MQ=255)                                                                      cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGtg                                                                                                                                      <  1:66297/184‑2 (MQ=255)                                                                      cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCa                                                                  >  1:654655/1‑251 (MQ=255)                                                                                                          cAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGTGtttttg                                                                                                                                >  1:438452/1‑153 (MQ=255)                                                                                                                              taaaTATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAatacg           <  1:512730/250‑2 (MQ=255)                                                                                                                               aaaTATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGtg                                                                                                                                      <  1:104600/128‑2 (MQ=255)                                                                                                                                       ttttttAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAc  <  1:895687/250‑1 (MQ=255)                                                                                                                                                                         agttaaAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGttaatt                                    >  1:234983/1‑183 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                   CAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATAC  >  1/1477620‑1478002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑