Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,613	T→A	intergenic (‑197/‑552)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,613	0	T	A	100.0%	67.5 / NA	26	intergenic (‑197/‑552)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/15);  total (11/15)

GTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAG  >  1/1477463‑1477797                                                                                                                                                       |                                                                                                                                                                                          cgttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAag                                                                                      <  1:396304/246‑1 (MQ=255)   ttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAagag                                                                                    >  1:896675/4‑251 (MQ=255)         caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                                                                 <  1:83881/245‑1 (MQ=255)         caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                                                                 <  1:489939/245‑1 (MQ=255)          aatatataGGATGATTTTCACCCAGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                                                                             <  1:299003/249‑2 (MQ=255)           atatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTCAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                                                                             <  1:437414/249‑2 (MQ=255)             atataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCa                                                                          >  1:384226/5‑251 (MQ=255)                  ggATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATaaa                                                                     >  1:277698/1‑251 (MQ=255)                    atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                                                                   <  1:706664/251‑1 (MQ=255)                    atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                                                                   <  1:592584/251‑1 (MQ=255)                      gatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTg                                                                 >  1:245994/1‑251 (MQ=255)                         tttCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGctc                                                              <  1:277556/251‑1 (MQ=255)                                       tGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaatata                                                 >  1:407675/1‑246 (MQ=255)                                           cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                    <  1:194500/241‑2 (MQ=255)                                           cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                    <  1:787465/241‑2 (MQ=255)                                             gTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATAc                                               <  1:488328/245‑1 (MQ=255)                                             gTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCCCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAgagatt                                                                                 >  1:320234/1‑210 (MQ=255)                                                  ggtaaacccacagcgGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGTCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                                                                   <  1:644614/208‑1 (MQ=255)                                                       acccacagcgGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                    <  1:408661/223‑2 (MQ=255)                                                                      cagaaagatcaaaaTGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                                                                   >  1:720724/11‑201 (MQ=255)                                                                                   aTGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTaaaaaa   >  1:552195/1‑251 (MQ=255)                                                                                    tGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAg  <  1:48135/251‑1 (MQ=255)                                                                                                          tgtTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGatg                                                                            >  1:333472/1‑155 (MQ=255)                                                                                                           gtTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGCTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                                                                 <  1:463876/151‑1 (MQ=255)                                                                                                                tCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGACACt                                                                                 >  1:129475/1‑146 (MQ=255)                                                                                                                                      gTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                                                                 >  1:408369/1‑124 (MQ=255)                                                                                                                                                       |                                                                                                                                                                                          GTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAG  >  1/1477463‑1477797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑