Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	224,174	C→T	V245V (GTG→GTA)	unknown ←	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	224,174	0	C	T	100.0%	111.0 / NA	41	V245V (GTG→GTA)	unknown	Mobile element protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/18);  total (23/18)

GAGTAAGGCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGCACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAATATCGCAAAAAAGCTTTCAATCACCGCATTATCATAGCAATTCCCT  >  1/223932‑224348                                                                                                                                                                                                                                                   |                                                                                                                                                                               gAGTAAGGCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCggg                                                                                                                                                                          >  1:607234/1‑249 (MQ=255) gAGTAAGGCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGc                                                                                                                                                                         <  1:236116/250‑1 (MQ=255) gAGTAAGGCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAACTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGc                                                                                                                                                                         <  1:440912/250‑1 (MQ=255)        gCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATcc                                                                                                                                                                  <  1:806637/250‑1 (MQ=255)        gCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATc                                                                                                                                                                   <  1:453880/249‑1 (MQ=255)                                    ggTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGt                                                                                                                                     >  1:374175/1‑251 (MQ=255)                                               gCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATTTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATacac                                                                                                                           <  1:105936/250‑1 (MQ=255)                                               gCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATacac                                                                                                                           <  1:71618/250‑1 (MQ=255)                                                     cAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAatat                                                                                                                    <  1:642818/251‑1 (MQ=255)                                                         gtatgtTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATAttctt                                                                                                                >  1:840306/1‑251 (MQ=255)                                                         gtatgtTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATAttctt                                                                                                                >  1:865996/1‑251 (MQ=255)                                                           atgtTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATc                                                                                                                                                                   >  1:40854/1‑198 (MQ=255)                                                                gggTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAAttt                                                                                                          >  1:165060/1‑250 (MQ=255)                                                                gggTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAAttt                                                                                                          >  1:584545/1‑250 (MQ=255)                                                                  gTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATcc                                                                                                                                                                  <  1:595151/192‑1 (MQ=255)                                                                      tGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATc                                                                                                                                                                   >  1:555247/1‑187 (MQ=255)                                                                            gACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAAtt                                                                                              >  1:139501/1‑250 (MQ=255)                                                                                  tacttacCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAAt                                                                                       >  1:359711/1‑251 (MQ=255)                                                                                          cGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGtat                                                                               <  1:706401/251‑1 (MQ=255)                                                                                            cTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATaa                                                                             >  1:841577/1‑251 (MQ=255)                                                                                              gTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGt                                                                           >  1:491354/1‑251 (MQ=255)                                                                                               tttCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTg                                                                          <  1:802534/251‑1 (MQ=255)                                                                                                      caacaTCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGt                                                                   >  1:99813/1‑251 (MQ=255)                                                                                                        acaTCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTaa                                                                 >  1:5179/1‑251 (MQ=255)                                                                                                        acaTCAATGATTCGGCGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTaa                                                                 >  1:755647/1‑251 (MQ=255)                                                                                                         caTCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGc                                                                                                                                                                         >  1:301797/1‑146 (MQ=255)                                                                                                            cAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTaaaaa                                                              >  1:427299/1‑250 (MQ=255)                                                                                                            cAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAac                                                             <  1:620748/251‑1 (MQ=255)                                                                                                                           cAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAata                                              >  1:481531/1‑251 (MQ=255)                                                                                                                           cAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAat                                               <  1:726662/250‑1 (MQ=255)                                                                                                                           cAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAat                                               <  1:841884/250‑1 (MQ=255)                                                                                                                              gaagGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGc                                                                                                                                                                         <  1:88769/125‑1 (MQ=255)                                                                                                                                agGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTaa                                                                                                                                <  1:430077/164‑1 (MQ=255)                                                                                                                                agGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAATATCGCa                                         >  1:640335/1‑251 (MQ=255)                                                                                                                                 ggTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATac                                                                                                                             <  1:56889/166‑1 (MQ=255)                                                                                                                                      aCCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAAt                                                                                                                               <  1:706583/159‑1 (MQ=255)                                                                                                                                            cGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATac                                                                                                                             >  1:665732/1‑155 (MQ=255)                                                                                                                                             gatcaaaGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATAttc                                                                                                                  >  1:602102/5‑165 (MQ=255)                                                                                                                                                         gTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAac                                                             >  1:135309/1‑206 (MQ=38)                                                                                                                                                            aaaaaaCGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAATATCGCAAAAAAGCTTTCAATCACCGCATTATCAt             <  1:216182/251‑1 (MQ=25)                                                                                                                                                                        gaATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGTACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAATATCGCAAAAAAGCTTTCAATCACCGCATTATCATAGCAATTCCCt  >  1:624546/1‑250 (MQ=11)                                                                                                                                                                                                                                                   |                                                                                                                                                                               GAGTAAGGCAAAACGATAAAGTCGTTTGAAATGCAGGTTTCCGACGGCGTGGCAATGTATGTTGGGTCATGATCCGACAATTACTTACCCGCTGTTTCAAACAACATCAATGATTCGGTGGGCAAGAAGGTATACCTTACGCTAAAAGTGCGGTCAAAAAACGTTGAGAATTTTGACCGCACTTTGATCTGCCCCCCAAAAGTTGGACAGGTTAGTTAACTTAAATATTGAGCTCGGTATTGCACCGGGCTTAATCCTTTTAAATCAAGTTTAATTCGTTCTTGGTTGTAATACACCAAATATTCTTCAATTTCCGCCTGTAATTCGGCAATCGAAGTATAAGTGCGTGAGTAAAAACACTCAGATTTTAATATCGCAAAAAAGCTTTCAATCACCGCATTATCATAGCAATTCCCT  >  1/223932‑224348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑