Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,513	A→G	intergenic (‑97/‑652)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,513	0	A	G	100.0%	76.0 / NA	28	intergenic (‑97/‑652)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/19);  total (9/19)

CATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC  >  1/1477275‑1477753                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 cgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCacagc                                                                                                                                                                                                                                      <  1:32361/249‑4 (MQ=255)                            cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGttttt                                                                                                                                                                                                           >  1:640682/9‑251 (MQ=255)                            cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCACTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGaaaga                                                                                                                                                                                                                        <  1:447021/230‑3 (MQ=255)                                            ttGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAGaaaggtg                                                                                                                                                                                           <  1:532977/251‑5 (MQ=255)                                             tGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGgtgt                                                                                                                                                                                         <  1:155591/251‑1 (MQ=255)                                                  tGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATc                                                                                                                                                                                    <  1:195459/251‑1 (MQ=255)                                                       ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCg                                                                                                                                                                               >  1:876007/1‑251 (MQ=255)                                                          ttGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGccc                                                                                                                                                                            <  1:156731/251‑3 (MQ=255)                                                                 ttgctCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTttaat                                                                                                                                                                                                       >  1:468258/5‑218 (MQ=255)                                                                                 gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGTACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATaaagaa                                                                                                                                                                 <  1:688211/239‑4 (MQ=255)                                                                                 gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGc                                                                                                                                                        <  1:13317/248‑1 (MQ=255)                                                                                                                                                                                             cgttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAag                                         <  1:396304/246‑1 (MQ=255)                                                                                                                                                                                               ttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAagag                                       >  1:896675/4‑251 (MQ=255)                                                                                                                                                                                                     caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                    <  1:489939/245‑1 (MQ=255)                                                                                                                                                                                                     caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                                    <  1:83881/245‑1 (MQ=255)                                                                                                                                                                                                      aatatataGGATGATTTTCACCCAGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                                <  1:299003/249‑2 (MQ=255)                                                                                                                                                                                                       atatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTCAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                                <  1:437414/249‑2 (MQ=255)                                                                                                                                                                                                         atataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCa                             >  1:384226/5‑251 (MQ=255)                                                                                                                                                                                                              ggATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATaaa                        >  1:277698/1‑251 (MQ=255)                                                                                                                                                                                                                atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                      <  1:592584/251‑1 (MQ=255)                                                                                                                                                                                                                atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt                      <  1:706664/251‑1 (MQ=255)                                                                                                                                                                                                                  gatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTg                    >  1:245994/1‑251 (MQ=255)                                                                                                                                                                                                                     tttCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGctc                 <  1:277556/251‑1 (MQ=255)                                                                                                                                                                                                                                   tGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaatata    >  1:407675/1‑246 (MQ=255)                                                                                                                                                                                                                                       cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat       <  1:787465/241‑2 (MQ=255)                                                                                                                                                                                                                                       cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat       <  1:194500/241‑2 (MQ=255)                                                                                                                                                                                                                                         gTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATAc  <  1:488328/245‑1 (MQ=255)                                                                                                                                                                                                                                         gTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCCCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAgagatt                                    >  1:320234/1‑210 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 CATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC  >  1/1477275‑1477753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑