Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,495	T→C	intergenic (‑79/‑670)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,495	0	T	C	97.0%	84.9 / NA	33	intergenic (‑79/‑670)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (11/21);  minor base A (0/1);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTC  >  1/1477257‑1477738                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    aTGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGcacgg                                                                                                                                                                                                                                         <  1:48612/251‑3 (MQ=255)         cTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                                                                                                                                                                 <  1:369172/251‑4 (MQ=255)                   cgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCacagc                                                                                                                                                                                                                       <  1:32361/249‑4 (MQ=255)                       cacaATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                                                                                                                                                                  <  1:776726/236‑3 (MQ=255)                             gcgcaCAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                                                                                                                                                                 >  1:685900/5‑228 (MQ=255)                                    gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGc                                                                                                                                                                                                                                             <  1:69061/194‑1 (MQ=255)                                    gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAgg                                                                                                                                                                                                                                   >  1:246810/19‑221 (MQ=255)                                              cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                                                                                                                                                                 >  1:498878/9‑211 (MQ=255)                                              cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGttttt                                                                                                                                                                                            >  1:640682/9‑251 (MQ=255)                                              cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCACTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGaaaga                                                                                                                                                                                                         <  1:447021/230‑3 (MQ=255)                                                      cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                                                                                                                                                                  <  1:144967/205‑3 (MQ=255)                                                              ttGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAGaaaggtg                                                                                                                                                                            <  1:532977/251‑5 (MQ=255)                                                               tGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGgtgt                                                                                                                                                                          <  1:155591/251‑1 (MQ=255)                                                                    tGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATc                                                                                                                                                                     <  1:195459/251‑1 (MQ=255)                                                                         ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCg                                                                                                                                                                >  1:876007/1‑251 (MQ=255)                                                                           gTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                                                                                                                                                                  <  1:303120/184‑3 (MQ=255)                                                                            ttGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGccc                                                                                                                                                             <  1:156731/251‑3 (MQ=255)                                                                                   ttgctCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAgg                                                                                                                                                                                                                                   >  1:626506/5‑174 (MQ=255)                                                                                   ttgctCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTttaat                                                                                                                                                                                        >  1:468258/5‑218 (MQ=255)                                                                                                   gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGTACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATaaagaa                                                                                                                                                  <  1:688211/239‑4 (MQ=255)                                                                                                   gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGc                                                                                                                                         <  1:13317/248‑1 (MQ=255)                                                                                                                                                                                                               cgttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAag                          <  1:396304/246‑1 (MQ=255)                                                                                                                                                                                                                 ttccagcaaTATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAagag                        >  1:896675/4‑251 (MQ=255)                                                                                                                                                                                                                       caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                     <  1:83881/245‑1 (MQ=255)                                                                                                                                                                                                                       caatatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACt                     <  1:489939/245‑1 (MQ=255)                                                                                                                                                                                                                        aatatataGGATGATTTTCACCCAGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                 <  1:299003/249‑2 (MQ=255)                                                                                                                                                                                                                         atatataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTCAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGat                 <  1:437414/249‑2 (MQ=255)                                                                                                                                                                                                                           atataGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCa              >  1:384226/5‑251 (MQ=255)                                                                                                                                                                                                                                ggATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATaaa         >  1:277698/1‑251 (MQ=255)                                                                                                                                                                                                                                  atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt       <  1:592584/251‑1 (MQ=255)                                                                                                                                                                                                                                  atgatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAAt       <  1:706664/251‑1 (MQ=255)                                                                                                                                                                                                                                    gatTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTg     >  1:245994/1‑251 (MQ=255)                                                                                                                                                                                                                                       tttCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGctc  <  1:277556/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTC  >  1/1477257‑1477738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑