| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 223,107 | G→A | D143D (GAC→GAT) | unknown ← | NAD‑dependent malic enzyme (EC 1.1.1.38) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 223,107 | 0 | G | A | 98.7% | 206.9 / NA | 74 | D143D (GAC→GAT) | unknown | NAD‑dependent malic enzyme (EC 1.1.1.38) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (34/39); minor base C (1/0); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTCAGGTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCGTCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAGTTGGTCG > 1/222872‑223344 | ttttCAGGTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATc < 1:646508/251‑1 (MQ=255) tttCAGGTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCa < 1:338025/251‑1 (MQ=255) gTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGAt < 1:18680/248‑1 (MQ=255) gTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATcc < 1:822992/250‑1 (MQ=255) ttCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt < 1:167104/251‑1 (MQ=255) ttCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt < 1:366565/251‑1 (MQ=255) ttCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt < 1:222720/251‑1 (MQ=255) tCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGGTCACGGCAAAGGCGCGGGCGGGGGCAATCCCTGCGGCAGCAGTGGAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGGTGCCGAGGAGTTCTTCCGCATCCGACACCCCAATCAGATc > 1:67789/1‑245 (MQ=255) gtcgtAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGAcaccac < 1:549854/222‑1 (MQ=255) tcgtAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATc < 1:93643/250‑1 (MQ=255) gtAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGGTCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGGTCACGGCAATGGCGCGGGCGGGGGCAATCCCTGCGGCAGCAGTGTAAAACGCCAGCTTGCCGACGGGAAAATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACCATCAGATCCACAACGTCCGCAccccgtct > 1:347507/1‑244 (MQ=255) aGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGGCAATCCCTGCGGCAGCAGTGTAAACCGCCCGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCCTCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAg > 1:144244/1‑251 (MQ=255) cGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAgg < 1:711614/250‑1 (MQ=255) cGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAgg < 1:485790/250‑1 (MQ=255) ccgcGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGGTCACGGCAATGGCGCGGGCGGGGGCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGGCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCAcc > 1:246961/1‑233 (MQ=255) cgcACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGa < 1:77430/251‑1 (MQ=255) tACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGa < 1:415249/251‑1 (MQ=255) aCCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt < 1:545921/200‑1 (MQ=255) gagaTATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGt > 1:73063/1‑251 (MQ=255) gaTATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACaa > 1:608451/1‑183 (MQ=255) atGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGata > 1:200535/1‑251 (MQ=255) tGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGatat > 1:619694/1‑251 (MQ=255) gATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt < 1:613669/186‑1 (MQ=255) tCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGa > 1:378957/1‑234 (MQ=255) tCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATa > 1:191697/1‑251 (MQ=255) tCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATa > 1:326100/1‑251 (MQ=255) cGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATaa < 1:709103/251‑1 (MQ=255) gAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCg > 1:385248/1‑251 (MQ=255) gggCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGAt < 1:262890/225‑1 (MQ=255) ggCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCAcgcg > 1:850796/1‑251 (MQ=255) cTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGCCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGa > 1:542453/1‑221 (MQ=255) tATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGAt > 1:262838/1‑159 (MQ=255) ccGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTcgcg < 1:375456/251‑1 (MQ=255) ggTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGa < 1:756815/186‑1 (MQ=255) gTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCAttttt > 1:266975/1‑251 (MQ=255) gCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCg > 1:596902/1‑250 (MQ=255) gCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGGCGCGACTCCATTTTTTAATCg > 1:185217/1‑250 (MQ=255) gTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCAcc < 1:209432/132‑1 (MQ=255) cccTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACggtcgggt < 1:564387/245‑1 (MQ=255) ccTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑acac < 1:596986/251‑1 (MQ=255) ccTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑acac < 1:504786/251‑1 (MQ=255) ccTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑acac < 1:818584/251‑1 (MQ=255) ccTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCAGCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑acac < 1:549219/251‑1 (MQ=255) cGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt < 1:370809/250‑1 (MQ=255) cGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt < 1:327526/250‑1 (MQ=255) cGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt < 1:400208/250‑1 (MQ=255) cGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt < 1:477511/250‑1 (MQ=255) gtgtAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACgg > 1:135781/1‑141 (MQ=255) gtgtAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGa > 1:810900/1‑153 (MQ=255) gtgtAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACggtcggggcg > 1:156730/1‑231 (MQ=255) gtgtAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt < 1:625281/242‑1 (MQ=255) gAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt > 1:269360/1‑218 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATc > 1:362268/1‑65 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACAt > 1:776378/1‑75 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTTATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACggt < 1:864481/199‑1 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAAt > 1:879714/1‑141 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACggtcgggtcg < 1:705107/206‑1 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑acac < 1:480951/211‑1 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTg > 1:194419/1‑251 (MQ=255) tatCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTg > 1:812719/1‑251 (MQ=255) tACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATAc < 1:338815/251‑1 (MQ=255) tACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATAc < 1:257031/251‑1 (MQ=255) tACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATAc < 1:686525/251‑1 (MQ=255) gTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGTACACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAg > 1:845017/1‑251 (MQ=255) gTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAAt > 1:857643/1‑204 (MQ=255) ttCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCAc < 1:456176/251‑1 (MQ=255) cGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGc > 1:655906/1‑67 (MQ=255) tCGCCGATGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCgg < 1:175206/249‑1 (MQ=255) aTGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAg < 1:469739/251‑1 (MQ=255) aTGCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAg > 1:732036/1‑251 (MQ=255) gCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGccc > 1:394444/1‑163 (MQ=255) gCCGAGGATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGGCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAGtt > 1:2307/1‑251 (MQ=255) ggATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAGGTGGTCg > 1:476712/1‑251 (MQ=255) gATTTCTTCCGCATCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATAc > 1:842852/1‑220 (MQ=255) | TTTTCAGGTATTCGGCAATCATCGCGTCGTAGCGTTCGCCGCGCACGCGGGCATGGCGGTTACCGAGATATGCGGGATCGCTGAGCAGGGCTTCGTTATCTGTGCCGACATCAAGGTTCACGGCAATGGCGCGGGCGGGGTCAATCCCTGCGGCAGCAGTGTAAACCGCCAGCTTGCCGACGGAAATATCTGTACCGTTCACGCCCCAGTCGCCGATGCCGAGGATTTCTTCCGCGTCCGACACCACAATCAGATCCACATCGTCCGCACCCAATCCCAAGGTTTCAAAGGATTCACGGATGGCTTCGGGATGGTTGATATCCAAATAAACCGCACGCGAACGGCGGTAGTCGCGACTCCATTTTTTAATCGCTTCGCCCACGGTCGGGTCGT‑‑ACACAATCGGCAGCATTTCCGCCAAATGGTCGGTCAGCAGTTTGTAATACAGCACTTCGTTGCGGTTGTGCAGTTGGTCG > 1/222872‑223344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |