Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,436	C→T	intergenic (‑20/‑729)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,436	0	C	T	100.0%	122.5 / NA	47	intergenic (‑20/‑729)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/32);  total (15/32)

CAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGC  >  1/1477200‑1477603                                                                                                                                                                                                                                             |                                                                                                                                                                        cAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCg                                                                                                                                                           >  1:164981/1‑251 (MQ=255)         aaaGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGc                                                                                                                                                   <  1:897207/251‑1 (MQ=255)                   caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                                                                                                          >  1:310007/1‑245 (MQ=255)                   caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                                                                                                          >  1:533243/1‑245 (MQ=255)                   caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                                              <  1:851079/246‑2 (MQ=255)                     ttAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccag                                                                                                                                       >  1:866114/1‑243 (MQ=255)                         tGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaa                                                                                                                                    <  1:583102/250‑12 (MQ=255)                              cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                                               <  1:514291/250‑17 (MQ=255)                              cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                                               <  1:210651/250‑17 (MQ=255)                                aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                                              <  1:896642/233‑2 (MQ=255)                                aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTCTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatatagg                                                                                                                            <  1:430183/251‑20 (MQ=255)                                  cTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                                          <  1:526905/251‑1 (MQ=255)                                          cGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                                              <  1:549858/223‑2 (MQ=255)                                            aTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAc                                                                                                                 <  1:823374/250‑1 (MQ=255)                                             tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                                               <  1:231436/235‑17 (MQ=255)                                             tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                                                                                               <  1:4973/251‑1 (MQ=255)                                             tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                                                                                               <  1:458737/251‑1 (MQ=255)                                                ttgaGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                                          >  1:856449/4‑237 (MQ=255)                                                          aTGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGcacgg                                                                                                  <  1:48612/251‑3 (MQ=255)                                                                  cTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                          <  1:369172/251‑4 (MQ=255)                                                                          ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                                          <  1:408800/207‑1 (MQ=255)                                                                          ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                                          <  1:257383/207‑1 (MQ=255)                                                                            cgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCacagc                                                                                <  1:32361/249‑4 (MQ=255)                                                                                cacaATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                           <  1:776726/236‑3 (MQ=255)                                                                                      gcgcaCAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                          >  1:685900/5‑228 (MQ=255)                                                                                             gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGc                                                                                                      <  1:69061/194‑1 (MQ=255)                                                                                             gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAgg                                                                                            >  1:246810/19‑221 (MQ=255)                                                                                                       cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAc                                                                                                                 >  1:138331/9‑191 (MQ=255)                                                                                                       cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                                          >  1:498878/9‑211 (MQ=255)                                                                                                       cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGttttt                                                     >  1:640682/9‑251 (MQ=255)                                                                                                       cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCACTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGaaaga                                                                  <  1:447021/230‑3 (MQ=255)                                                                                                               cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                                               >  1:218064/1‑153 (MQ=255)                                                                                                               cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagc                                                                                                                                      >  1:360669/1‑153 (MQ=255)                                                                                                               cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                           <  1:144967/205‑3 (MQ=255)                                                                                                                      gTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                                               <  1:522133/162‑17 (MQ=255)                                                                                                                       ttGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAGaaaggtg                                     <  1:532977/251‑5 (MQ=255)                                                                                                                        tGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGgtgt                                   <  1:155591/251‑1 (MQ=255)                                                                                                                             tGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATc                              <  1:195459/251‑1 (MQ=255)                                                                                                                                  ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccag                                                                                                                                       <  1:302464/142‑9 (MQ=255)                                                                                                                                  ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCg                         >  1:876007/1‑251 (MQ=255)                                                                                                                                    gTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                                           <  1:303120/184‑3 (MQ=255)                                                                                                                                     ttGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGccc                      <  1:156731/251‑3 (MQ=255)                                                                                                                                            ttgctCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAgg                                                                                            >  1:626506/5‑174 (MQ=255)                                                                                                                                            ttgctCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTttaat                                                 >  1:468258/5‑218 (MQ=255)                                                                                                                                                            gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGTACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATaaagaa           <  1:688211/239‑4 (MQ=255)                                                                                                                                                            gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                                          <  1:591108/129‑1 (MQ=255)                                                                                                                                                            gCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGc  <  1:13317/248‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                        CAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGC  >  1/1477200‑1477603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑