Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,477,342 T→C E25E (GAA→GAG)  unknown ← Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,477,3420TC100.0% 189.0 / NA 66E25E (GAA→GAG) unknownMetal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/41);  total (25/41)

GGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTC  >  1/1477109‑1477583
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
ggTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGt                                                                                                                                                                                                                                  >  1:395898/1‑251 (MQ=255)
                                  cTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATc                                                                                                                                                                                                                >  1:166424/1‑235 (MQ=255)
                                     gacgacAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTaaa                                                                                                                                                                                             >  1:546676/1‑251 (MQ=255)
                                             aTCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCaataat                                                                                                                                                                                     <  1:190239/251‑1 (MQ=255)
                                              tCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatc                                                                                                                                                                                    <  1:275563/251‑1 (MQ=255)
                                                   aaaTCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatcatttt                                                                                                                                                                               >  1:843914/1‑248 (MQ=255)
                                                      tCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAcc                                                                                                                                                                            <  1:2257/251‑1 (MQ=255)
                                                       cGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc                                                                                                                                                                           <  1:282504/251‑1 (MQ=255)
                                                        gCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc                                                                                                                                                                           <  1:121083/250‑1 (MQ=255)
                                                                       ttCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTcccata                                                                                                                                                             <  1:764983/248‑4 (MQ=255)
                                                                        tCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                                                                          <  1:643511/251‑1 (MQ=255)
                                                                        tCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                                                                          <  1:462168/251‑1 (MQ=255)
                                                                         ccACTTCATCAGGGGATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAtt                                                                                                                                                         <  1:770484/251‑1 (MQ=255)
                                                                          cACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                                                                          >  1:127274/1‑249 (MQ=255)
                                                                           aCTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTc                                                                                                                                                        >  1:382044/1‑250 (MQ=255)
                                                                            cTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCAt                                                                                                                                                      >  1:545251/1‑251 (MQ=255)
                                                                            cTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCAt                                                                                                                                                      >  1:888932/1‑251 (MQ=255)
                                                                              tcatcaGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAcc                                                                                                                                                                            <  1:80159/227‑1 (MQ=255)
                                                                                      gCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTc                                                                                                                                                                                                                   <  1:279376/180‑1 (MQ=255)
                                                                                           cAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCg                                                                                                                                       >  1:164981/1‑251 (MQ=255)
                                                                                                gggAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAAttt                                                                                                                                                                                                                                            >  1:405567/1‑145 (MQ=255)
                                                                                                gggAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGcc                                                                                                                                                                                                                         >  1:673872/1‑164 (MQ=255)
                                                                                                   aaaGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGc                                                                                                                               <  1:897207/251‑1 (MQ=255)
                                                                                                             caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTc                                                                                                                                                        <  1:195714/216‑1 (MQ=255)
                                                                                                             caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                                                                                      >  1:310007/1‑245 (MQ=255)
                                                                                                             caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                                                                                      >  1:533243/1‑245 (MQ=255)
                                                                                                             caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                          <  1:851079/246‑2 (MQ=255)
                                                                                                               ttAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatc                                                                                                                                                                                    >  1:884109/1‑186 (MQ=255)
                                                                                                               ttAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccag                                                                                                                   >  1:866114/1‑243 (MQ=255)
                                                                                                                   tGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaa                                                                                                                <  1:583102/250‑12 (MQ=255)
                                                                                                                        cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                           <  1:210651/250‑17 (MQ=255)
                                                                                                                        cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                           <  1:514291/250‑17 (MQ=255)
                                                                                                                          aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                          <  1:896642/233‑2 (MQ=255)
                                                                                                                          aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTCTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatatagg                                                                                                        <  1:430183/251‑20 (MQ=255)
                                                                                                                            cTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                      <  1:526905/251‑1 (MQ=255)
                                                                                                                                    cGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                                                                          <  1:549858/223‑2 (MQ=255)
                                                                                                                                      aTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAc                                                                                             <  1:823374/250‑1 (MQ=255)
                                                                                                                                       tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                           <  1:231436/235‑17 (MQ=255)
                                                                                                                                       tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                                                                           <  1:4973/251‑1 (MQ=255)
                                                                                                                                       tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                                                                           <  1:458737/251‑1 (MQ=255)
                                                                                                                                          ttgaGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                      >  1:856449/4‑237 (MQ=255)
                                                                                                                                                    aTGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGcacgg                                                                              <  1:48612/251‑3 (MQ=255)
                                                                                                                                                            cTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                      <  1:369172/251‑4 (MQ=255)
                                                                                                                                                                    ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                      <  1:257383/207‑1 (MQ=255)
                                                                                                                                                                    ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                                                                                      <  1:408800/207‑1 (MQ=255)
                                                                                                                                                                      cgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCacagc                                                            <  1:32361/249‑4 (MQ=255)
                                                                                                                                                                          cacaATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                       <  1:776726/236‑3 (MQ=255)
                                                                                                                                                                                gcgcaCAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                      >  1:685900/5‑228 (MQ=255)
                                                                                                                                                                                       gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGc                                                                                  <  1:69061/194‑1 (MQ=255)
                                                                                                                                                                                       gtcatttccacttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAgg                                                                        >  1:246810/19‑221 (MQ=255)
                                                                                                                                                                                                 cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAc                                                                                             >  1:138331/9‑191 (MQ=255)
                                                                                                                                                                                                 cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta                                                                      >  1:498878/9‑211 (MQ=255)
                                                                                                                                                                                                 cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGttttt                                 >  1:640682/9‑251 (MQ=255)
                                                                                                                                                                                                 cttcatcacTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCACTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGaaaga                                              <  1:447021/230‑3 (MQ=255)
                                                                                                                                                                                                        acTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                                                                          >  1:486571/2‑123 (MQ=255)
                                                                                                                                                                                                         cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                           >  1:218064/1‑153 (MQ=255)
                                                                                                                                                                                                         cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagc                                                                                                                  >  1:360669/1‑153 (MQ=255)
                                                                                                                                                                                                         cTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                       <  1:144967/205‑3 (MQ=255)
                                                                                                                                                                                                                gTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                                                                           <  1:522133/162‑17 (MQ=255)
                                                                                                                                                                                                                 ttGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAGaaaggtg                 <  1:532977/251‑5 (MQ=255)
                                                                                                                                                                                                                  tGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGgtgt               <  1:155591/251‑1 (MQ=255)
                                                                                                                                                                                                                       tGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATc          <  1:195459/251‑1 (MQ=255)
                                                                                                                                                                                                                            ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccag                                                                                                                   <  1:302464/142‑9 (MQ=255)
                                                                                                                                                                                                                            ccgTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCg     >  1:876007/1‑251 (MQ=255)
                                                                                                                                                                                                                              gTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt                                                                       <  1:303120/184‑3 (MQ=255)
                                                                                                                                                                                                                               ttGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGccc  <  1:156731/251‑3 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
GGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTC  >  1/1477109‑1477583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑