Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,309	G→A	S36S (AGC→AGT)	unknown ←	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,309	0	G	A	100.0%	147.9 / NA	57	S36S (AGC→AGT)	unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/37);  total (20/37)

AACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGAC  >  1/1477068‑1477525                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                         aaCTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGt                                                                                                                                                                                                                 <  1:67083/251‑1 (MQ=255)        gTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCa                                                                                                                                                                                                             >  1:876441/1‑248 (MQ=255)             ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTgccgc                                                                                                                                                                                                      >  1:141467/1‑250 (MQ=255)                 caTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAGATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGGTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTg                                                                                                                                                                                                 <  1:216902/251‑1 (MQ=255)                       tgCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCAt                                                                                                                                                                                            <  1:797760/250‑1 (MQ=255)                         cccAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTgc                                                                                                                                                                                         <  1:174269/251‑2 (MQ=255)                                          ggTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGt                                                                                                                                                                        >  1:395898/1‑251 (MQ=255)                                               ctcttgctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTg                                                                                                                                                                                                          <  1:691261/212‑1 (MQ=255)                                                                    cTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGcc                                                                                                                                                                                               >  1:313794/1‑202 (MQ=255)                                                                            cTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATc                                                                                                                                                      >  1:166424/1‑235 (MQ=255)                                                                               gacgacAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTaaa                                                                                                                                   >  1:546676/1‑251 (MQ=255)                                                                                       aTCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCaataat                                                                                                                           <  1:190239/251‑1 (MQ=255)                                                                                        tCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatc                                                                                                                          <  1:275563/251‑1 (MQ=255)                                                                                             aaaTCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatcatttt                                                                                                                     >  1:843914/1‑248 (MQ=255)                                                                                                tCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAcc                                                                                                                  <  1:2257/251‑1 (MQ=255)                                                                                                 cGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc                                                                                                                 <  1:282504/251‑1 (MQ=255)                                                                                                  gCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc                                                                                                                 <  1:121083/250‑1 (MQ=255)                                                                                                                 ttCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTcccata                                                                                                   <  1:764983/248‑4 (MQ=255)                                                                                                                  tCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                <  1:643511/251‑1 (MQ=255)                                                                                                                  tCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                <  1:462168/251‑1 (MQ=255)                                                                                                                   ccACTTCATCAGGGGATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAtt                                                                                               <  1:770484/251‑1 (MQ=255)                                                                                                                    cACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAAt                                                                                                >  1:127274/1‑249 (MQ=255)                                                                                                                     aCTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTc                                                                                              >  1:382044/1‑250 (MQ=255)                                                                                                                      cTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCAt                                                                                            >  1:888932/1‑251 (MQ=255)                                                                                                                      cTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCAt                                                                                            >  1:545251/1‑251 (MQ=255)                                                                                                                        tcatcaGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAcc                                                                                                                  <  1:80159/227‑1 (MQ=255)                                                                                                                              gggCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTg                                                                                                                                                                                                               <  1:275061/128‑1 (MQ=255)                                                                                                                                gCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTc                                                                                                                                                         <  1:279376/180‑1 (MQ=255)                                                                                                                                     cAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCg                                                                             >  1:164981/1‑251 (MQ=255)                                                                                                                                          gggAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAAttt                                                                                                                                                                                  >  1:405567/1‑145 (MQ=255)                                                                                                                                          gggAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGcc                                                                                                                                                               >  1:673872/1‑164 (MQ=255)                                                                                                                                             aaaGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGc                                                                     <  1:897207/251‑1 (MQ=255)                                                                                                                                                       caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTc                                                                                              <  1:195714/216‑1 (MQ=255)                                                                                                                                                       caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                            >  1:533243/1‑245 (MQ=255)                                                                                                                                                       caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttc                                                            >  1:310007/1‑245 (MQ=255)                                                                                                                                                       caTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                <  1:851079/246‑2 (MQ=255)                                                                                                                                                         ttAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatc                                                                                                                          >  1:884109/1‑186 (MQ=255)                                                                                                                                                         ttAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccag                                                         >  1:866114/1‑243 (MQ=255)                                                                                                                                                             tGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaa                                                      <  1:583102/250‑12 (MQ=255)                                                                                                                                                                  cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                 <  1:514291/250‑17 (MQ=255)                                                                                                                                                                  cGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                 <  1:210651/250‑17 (MQ=255)                                                                                                                                                                    aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                <  1:896642/233‑2 (MQ=255)                                                                                                                                                                    aTCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTCTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatatagg                                              <  1:430183/251‑20 (MQ=255)                                                                                                                                                                      cTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                            <  1:526905/251‑1 (MQ=255)                                                                                                                                                                              cGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGac                                                                <  1:549858/223‑2 (MQ=255)                                                                                                                                                                                aTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAc                                   <  1:823374/250‑1 (MQ=255)                                                                                                                                                                                 tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGacgttccagcaatatat                                                 <  1:231436/235‑17 (MQ=255)                                                                                                                                                                                 tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                 <  1:458737/251‑1 (MQ=255)                                                                                                                                                                                 tAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCAccc                                 <  1:4973/251‑1 (MQ=255)                                                                                                                                                                                    ttgaGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                            >  1:856449/4‑237 (MQ=255)                                                                                                                                                                                              aTGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGcacgg                    <  1:48612/251‑3 (MQ=255)                                                                                                                                                                                                      cTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta            <  1:369172/251‑4 (MQ=255)                                                                                                                                                                                                              ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                            <  1:257383/207‑1 (MQ=255)                                                                                                                                                                                                              ctcgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGgat                                            <  1:408800/207‑1 (MQ=255)                                                                                                                                                                                                                cgtCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAGGTAAACCCacagc  <  1:32361/249‑4 (MQ=255)                                                                                                                                                                                                                    cacaATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggt             <  1:776726/236‑3 (MQ=255)                                                                                                                                                                                                                          gcgcaCAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTACCCATGTTGCTCCCATAAATTCATTGCCATTTCCAACCGCACTTTGCACGACGTTCCAGCAATATATAGGATGATTTTCACCCCGCAGTTGCACGGTAAAggta            >  1:685900/5‑228 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                         AACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCCATGTTGCTCCCCTAAATTCATCGCCATTTCCGACCGCATTTTGCACGAGTTTACAGCAGTATACAGGATGATTTTCACCCTGCAATTGCACAGTAAAGATAAAACCACGAC  >  1/1477068‑1477525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑