Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,180	C→T	E79E (GAG→GAA)	unknown ←	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,180	0	C	T	100.0%	193.9 / NA	72	E79E (GAG→GAA)	unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/37);  total (35/37)

CCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCC  >  1/1476941‑1477414                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           ccGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                                                                                 <  1:867279/251‑1 (MQ=255) ccGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                                                                                 <  1:354887/251‑1 (MQ=255)        tttCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCAt                                                                                                                                                                                                                          >  1:865091/1‑251 (MQ=255)                      cTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGa                                                                                                                                                                                                            >  1:588628/1‑251 (MQ=255)                       tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                                                                           <  1:521505/251‑1 (MQ=255)                       tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                                                                           <  1:40111/251‑1 (MQ=255)                       tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                                                                           <  1:630872/251‑1 (MQ=255)                         aGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCa                                                                                                                                                                                                                       <  1:793496/237‑1 (MQ=255)                          gATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAg                                                                                                                                                                                                         >  1:897916/1‑250 (MQ=255)                          gATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAg                                                                                                                                                                                                         >  1:210376/1‑250 (MQ=255)                                gATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcctc                                                                                                                                                                                                                                 >  1:211345/1‑217 (MQ=255)                                    gACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATTGCCCAATAACGGCAGTTCCACTTCATCAGGGGATTCAAAAGGGAAAGACAGCACATTAGTgg                                                                                                                                                                                              >  1:350772/1‑251 (MQ=255)                                     aCAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATGTGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgg                                                                                                                                                                                              <  1:85136/250‑1 (MQ=255)                                      cAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgggg                                                                                                                                                                                            <  1:59905/251‑1 (MQ=255)                                          gccaagccCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGc                                                                                                                                                                                                                            >  1:302764/1‑215 (MQ=255)                                                 ccTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGcc                                                                                                                                                                                 <  1:576486/251‑1 (MQ=255)                                                    aTCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAg                                                                                                                                                                                                                               >  1:62584/1‑202 (MQ=255)                                                    aTCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTCCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACg                                                                                                                                                                              >  1:3532/1‑251 (MQ=255)                                                             tGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAAttgag                                                                                                                                                                     <  1:390134/251‑4 (MQ=255)                                                                 tttCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgggg                                                                                                                                                                                            <  1:831430/224‑1 (MQ=255)                                                                           ttCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTgg                                                                                                                                                        >  1:588389/1‑250 (MQ=255)                                                                           ttCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTgg                                                                                                                                                        >  1:189549/1‑250 (MQ=255)                                                                                  tcctccGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccat                                                                                                                                                <  1:894455/251‑3 (MQ=255)                                                                                         cTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTcc                                                                                                                                         <  1:676804/251‑1 (MQ=255)                                                                                           tCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCca                                                                                                                                        >  1:440046/1‑250 (MQ=255)                                                                                             gTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAAttg                                                                                                                                                                       >  1:871402/1‑216 (MQ=255)                                                                                              tCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                                                                                     <  1:196969/250‑1 (MQ=255)                                                                                                  cTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                                                                                 <  1:422596/154‑1 (MQ=255)                                                                                                     cAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGcac                                                                                                                                                                                                      >  1:120992/1‑178 (MQ=255)                                                                                                         atGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCa                                                                                                                         <  1:726057/251‑1 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAg                                                                                                                                                                                                 >  1:510259/1‑177 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATg                                                                                                                                                             <  1:365236/213‑1 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCcac                                                                                                                                       <  1:760957/235‑1 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                                                                       >  1:646851/1‑250 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                                                                       >  1:318178/1‑250 (MQ=255)                                                                                                           gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                                                                       >  1:662514/1‑250 (MQ=255)                                                                                                            aTCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAttt                                                                                                                      <  1:141228/251‑3 (MQ=255)                                                                                                             tCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAggg                                                                                                                                                                                                                             <  1:533433/147‑1 (MQ=255)                                                                                                              cATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                                                                                    <  1:769070/251‑5 (MQ=255)                                                                                                                tAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGGTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttccac                                                                                                                  <  1:782267/251‑7 (MQ=255)                                                                                                                     gaGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACGTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttccac                                                                                                                  <  1:576827/245‑7 (MQ=255)                                                                                                                     gaGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccatc                                                                                                                                               >  1:778042/2‑214 (MQ=255)                                                                                                                                aaCTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGt                                                                                                  <  1:67083/251‑1 (MQ=255)                                                                                                                                       gTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCt                                                                                                                                                                                      >  1:81299/1‑160 (MQ=255)                                                                                                                                       gTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCa                                                                                              >  1:876441/1‑248 (MQ=255)                                                                                                                                            ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccat                                                                                                                                                >  1:298273/1‑191 (MQ=255)                                                                                                                                            ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                                                                                     <  1:349286/204‑1 (MQ=255)                                                                                                                                            ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcca                                                                                                                   >  1:147480/1‑217 (MQ=255)                                                                                                                                            ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTgccgc                                                                                       >  1:141467/1‑250 (MQ=255)                                                                                                                                                caTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAGATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGGTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTg                                                                                  <  1:216902/251‑1 (MQ=255)                                                                                                                                                 aTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                                                                                    >  1:863527/1‑212 (MQ=255)                                                                                                                                                 aTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTcatc                                                                                                            <  1:674036/224‑4 (MQ=255)                                                                                                                                                      tgCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCAt                                                                             <  1:797760/250‑1 (MQ=255)                                                                                                                                                        cccAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACtt                                                                                                                >  1:761752/1‑213 (MQ=255)                                                                                                                                                        cccAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTgc                                                                          <  1:174269/251‑2 (MQ=255)                                                                                                                                                                         ggTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGt                                                         >  1:395898/1‑251 (MQ=255)                                                                                                                                                                              ctcttgctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                                                                                    >  1:384011/1‑183 (MQ=255)                                                                                                                                                                              ctcttgctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTg                                                                                           <  1:691261/212‑1 (MQ=255)                                                                                                                                                                                    ctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                                                                                     <  1:533413/164‑1 (MQ=255)                                                                                                                                                                                           gcctCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAAttg                                                                                                                                                                       >  1:847556/4‑122 (MQ=255)                                                                                                                                                                                                   cTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGcc                                                                                >  1:313794/1‑202 (MQ=255)                                                                                                                                                                                                     ccaccaCTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGTGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                                                                                     >  1:644194/1‑147 (MQ=255)                                                                                                                                                                                                     ccaccaCTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGt                                                                                                                           >  1:328432/1‑157 (MQ=255)                                                                                                                                                                                                        ccacTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGcc                                                                                                                                                                                 <  1:396210/100‑1 (MQ=255)                                                                                                                                                                                                           cTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATc                                       >  1:166424/1‑235 (MQ=255)                                                                                                                                                                                                              gacgacAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTaaa                    >  1:546676/1‑251 (MQ=255)                                                                                                                                                                                                                      aTCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCaataat            <  1:190239/251‑1 (MQ=255)                                                                                                                                                                                                                       tCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatc           <  1:275563/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaTCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAatcatttt      >  1:843914/1‑248 (MQ=255)                                                                                                                                                                                                                               tCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAcc   <  1:2257/251‑1 (MQ=255)                                                                                                                                                                                                                                cGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc  <  1:282504/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGTCGGCAAGCCATTTTCATCTGCGCTGGCGATTTGTAAATCAATAATCATTTTAccc  <  1:121083/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           CCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGTCGGCAAGCCATTTTCATCTGCGCAGGCGATTTGTAAATCAATAATCATCTTACCC  >  1/1476941‑1477414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑