Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,129	T→C	E96E (GAA→GAG)	unknown ←	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,129	0	T	C	100.0%	186.2 / NA	68	E96E (GAA→GAG)	unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

CAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGT  >  1/1476895‑1477359                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       cAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCcaccac                                                                                                                                                                                                                          <  1:143813/250‑1 (MQ=255)       tGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACga                                                                                                                                                                                                                   >  1:9290/1‑250 (MQ=255)         cccGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACgac                                                                                                                                                                                                                  <  1:542021/250‑3 (MQ=255)                                  tagttttacactttCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCACTGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCTCTCCACCACTTGACGACAAATCACCAAATCGCCCAATaacgg                                                                                                                                                                                         >  1:433959/13‑246 (MQ=255)                                    gttttacactttCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATaacggcag                                                                                                                                                                                      >  1:200994/11‑244 (MQ=255)                                            ctttCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATc                                                                                                                                                                                                             >  1:28042/3‑220 (MQ=255)                                                ccGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                          <  1:354887/251‑1 (MQ=255)                                                ccGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                          <  1:867279/251‑1 (MQ=255)                                                       tttCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCAt                                                                                                                                                                   >  1:865091/1‑251 (MQ=255)                                                                     cTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGa                                                                                                                                                     >  1:588628/1‑251 (MQ=255)                                                                      tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                    <  1:40111/251‑1 (MQ=255)                                                                      tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                    <  1:630872/251‑1 (MQ=255)                                                                      tCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGAc                                                                                                                                                    <  1:521505/251‑1 (MQ=255)                                                                        aGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCa                                                                                                                                                                <  1:793496/237‑1 (MQ=255)                                                                         gATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAg                                                                                                                                                  >  1:897916/1‑250 (MQ=255)                                                                         gATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAg                                                                                                                                                  >  1:210376/1‑250 (MQ=255)                                                                               gATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcctc                                                                                                                                                                          >  1:211345/1‑217 (MQ=255)                                                                                   gACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATTGCCCAATAACGGCAGTTCCACTTCATCAGGGGATTCAAAAGGGAAAGACAGCACATTAGTgg                                                                                                                                       >  1:350772/1‑251 (MQ=255)                                                                                    aCAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATGTGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgg                                                                                                                                       <  1:85136/250‑1 (MQ=255)                                                                                     cAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgggg                                                                                                                                     <  1:59905/251‑1 (MQ=255)                                                                                         gccaagccCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGc                                                                                                                                                                     >  1:302764/1‑215 (MQ=255)                                                                                                ccTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGcc                                                                                                                          <  1:576486/251‑1 (MQ=255)                                                                                                   aTCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACgac                                                                                                                                                                                                                  <  1:887233/160‑3 (MQ=255)                                                                                                   aTCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAg                                                                                                                                                                        >  1:62584/1‑202 (MQ=255)                                                                                                   aTCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTCCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACg                                                                                                                       >  1:3532/1‑251 (MQ=255)                                                                                                            tGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAAttgag                                                                                                              <  1:390134/251‑4 (MQ=255)                                                                                                                tttCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTgggg                                                                                                                                     <  1:831430/224‑1 (MQ=255)                                                                                                                          ttCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTgg                                                                                                 >  1:588389/1‑250 (MQ=255)                                                                                                                          ttCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTgg                                                                                                 >  1:189549/1‑250 (MQ=255)                                                                                                                                 tcctccGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccat                                                                                         <  1:894455/251‑3 (MQ=255)                                                                                                                                        cTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTcc                                                                                  <  1:676804/251‑1 (MQ=255)                                                                                                                                          tCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCca                                                                                 >  1:440046/1‑250 (MQ=255)                                                                                                                                            gTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAAttg                                                                                                                >  1:871402/1‑216 (MQ=255)                                                                                                                                             tCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                              <  1:196969/250‑1 (MQ=255)                                                                                                                                                 cTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTtcatc                                                                                                                                                                          <  1:422596/154‑1 (MQ=255)                                                                                                                                                    cAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGcac                                                                                                                                               >  1:120992/1‑178 (MQ=255)                                                                                                                                                        atGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCa                                                                  <  1:726057/251‑1 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAg                                                                                                                                          >  1:510259/1‑177 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATg                                                                                                      <  1:365236/213‑1 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCcac                                                                                <  1:760957/235‑1 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                >  1:318178/1‑250 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                >  1:662514/1‑250 (MQ=255)                                                                                                                                                          gATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtt                                                                >  1:646851/1‑250 (MQ=255)                                                                                                                                                           aTCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAttt                                                               <  1:141228/251‑3 (MQ=255)                                                                                                                                                            tCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAggg                                                                                                                                                                      <  1:533433/147‑1 (MQ=255)                                                                                                                                                             cATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                             <  1:769070/251‑5 (MQ=255)                                                                                                                                                               tAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGGTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttccac                                                           <  1:782267/251‑7 (MQ=255)                                                                                                                                                                    gaGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACGTTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttccac                                                           <  1:576827/245‑7 (MQ=255)                                                                                                                                                                    gaGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccatc                                                                                        >  1:778042/2‑214 (MQ=255)                                                                                                                                                                               aaCTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGt                                           <  1:67083/251‑1 (MQ=255)                                                                                                                                                                                      gTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCt                                                                                                                               >  1:81299/1‑160 (MQ=255)                                                                                                                                                                                      gTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCa                                       >  1:876441/1‑248 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCcaccac                                                                                                                                                                                                                          <  1:382014/64‑1 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATc                                                                                                                                                                                                             >  1:21125/1‑77 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTccat                                                                                         >  1:298273/1‑191 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                              <  1:349286/204‑1 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcca                                                            >  1:147480/1‑217 (MQ=255)                                                                                                                                                                                           ccaccaTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTgccgc                                >  1:141467/1‑250 (MQ=255)                                                                                                                                                                                               caTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAGATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGGTAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTg                           <  1:216902/251‑1 (MQ=255)                                                                                                                                                                                                aTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                             >  1:863527/1‑212 (MQ=255)                                                                                                                                                                                                aTGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTcatc                                                     <  1:674036/224‑4 (MQ=255)                                                                                                                                                                                                     tgCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCAt                      <  1:797760/250‑1 (MQ=255)                                                                                                                                                                                                       cccAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACtt                                                         >  1:761752/1‑213 (MQ=255)                                                                                                                                                                                                       cccAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTgc                   <  1:174269/251‑2 (MQ=255)                                                                                                                                                                                                                        ggTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTGCCGCCGTTGCCCATTGCTCAATTTGCTCCGCCGt  >  1:395898/1‑251 (MQ=255)                                                                                                                                                                                                                             ctcttgctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCAtttcc                                                             >  1:384011/1‑183 (MQ=255)                                                                                                                                                                                                                             ctcttgctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACAATGCGCACAGTCATTTCCACTTCATCACTTTGCGGTTGCACTg                                    <  1:691261/212‑1 (MQ=255)                                                                                                                                                                                                                                   ctcttgAGCCTCGCGCTCCACCACTTGACGACAAATCACCAAATCGCCCAATAACGGCAGTTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTGAGATTTAATGCGTGGCTTTCCATCTCGTCCACaa                                                                              <  1:533413/164‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       CAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGCAAATCACCAAATCGCCCAATAGCAGCAGCTCCACTTCATCAGGGCATTCAAAAGGGAAAGACAGCACATTAGTGGGGCGATCTTTGCCACGATAATTAAGATTTAATGCGTGGCTTTCCGTCTCATCCACAATGCGTACAGTCATCTCAACTTCCCCGCTTTGCGGTTGCACTGCCGCCGTTGCCCATTGTTCAATTTGCGCTGCCGT  >  1/1476895‑1477359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑