Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,938	A→T	intergenic (‑156/+14)	unknown ← / ← unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,938	0	A	T	100.0%	184.2 / NA	66	intergenic (‑156/+14)	unknown/unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (30/36);  total (30/36)

CGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGC  >  1/1476711‑1477151                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      cGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATa                                                                                                                                                                                        <  1:711569/251‑1 (MQ=255)        ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTT‑AGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGCCTTTTTCTTACTTCTCGCTCAGATACGGATc                                                                                                                                                                                  >  1:43973/1‑250 (MQ=255)        ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAATAAGTATTTTTTTTACATGATATTCC‑TTCAGGTTGAAAAAAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAA‑‑‑‑ACAT‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGGTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTTCGACTTTTTTTTACTTCTCGCTCAGATACGGATCg                                                                                                                                                                                 >  1:386685/1‑251 (MQ=255)         gataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                                                   <  1:444596/248‑1 (MQ=255)               aaccggcacAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGGATTTTTTTTACATGATATTTC‑TTCAGGGTGGAAATAGATTAATTATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAaag                                                                                                                                                                           >  1:189979/9‑250 (MQ=255)               aaccggcacAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                                                   >  1:580545/9‑242 (MQ=255)                                           cGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTCTCCGACTTTTTCTTACTTCTCGCTCAGATAc                                                                                                                                                                                       <  1:42256/210‑1 (MQ=255)                                                                gtaagtatttttttaacatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCAttt                                                                                                                                   >  1:187543‑M2/108‑251 (MQ=255)                                                                    gtatttttttaacatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTtcct                                                                                                                                >  1:702070‑M2/104‑250 (MQ=255)                                                                               acatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCAt                                                                                                                    >  1:842769‑M2/93‑251 (MQ=255)                                                                                ccttcaGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                                                   >  1:391927/6‑178 (MQ=255)                                                                                    atattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTtcc                                                                                                                                 >  1:333155‑M2/88‑233 (MQ=255)                                                                                    atattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCa                                                                                                                     <  1:204574‑M2/158‑1 (MQ=255)                                                                                     tattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCaa                                                                                                              >  1:426788‑M2/87‑251 (MQ=255)                                                                                        tccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                                           <  1:690648‑M2/168‑1 (MQ=255)                                                                                        tccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                                           <  1:722156‑M2/168‑1 (MQ=255)                                                                                           ttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGAt                                                                                                        <  1:446602‑M2/171‑1 (MQ=255)                                                                                            tcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATc                                                                                                       <  1:471959‑M2/172‑1 (MQ=255)                                                                                                aGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                                                   >  1:460939/1‑162 (MQ=255)                                                                                                  tgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAcc                                                                                                 <  1:472576‑M2/178‑1 (MQ=255)                                                                                                      aatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGt                                                                                             <  1:66273‑M2/182‑1 (MQ=255)                                                                                                          gattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                                                  >  1:855640‑M2/66‑242 (MQ=255)                                                                                                          gattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAAt                                                                                         >  1:761325‑M2/66‑251 (MQ=255)                                                                                                             atccggtcccttccgtgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAaccg                                                                                                <  1:333148‑M2/179‑2 (MQ=255)                                                                                                              aatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCaa                                                                                     >  1:845801‑M2/62‑251 (MQ=255)                                                                                                               atcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                                                  <  1:150714‑M2/177‑1 (MQ=255)                                                                                                                    gtcccgctgcagcaaccttaaaagcctgaattacggtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGc                                                                                >  1:618874‑M2/56‑250 (MQ=255)                                                                                                                             gcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                                                  >  1:127641‑M2/47‑223 (MQ=255)                                                                                                                                   accttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgtg                                                                <  1:797600‑M2/211‑1 (MQ=255)                                                                                                                                   accttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgtg                                                                <  1:115923‑M2/211‑1 (MQ=255)                                                                                                                                    ccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATaa                                                                                                                                                           <  1:366629‑M2/120‑1 (MQ=255)                                                                                                                                     cttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                                      <  1:897391‑M2/205‑1 (MQ=255)                                                                                                                                        aaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGccc                                                           <  1:530022‑M2/216‑1 (MQ=255)                                                                                                                                           agcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgt                                                                 >  1:246222‑M2/33‑242 (MQ=255)                                                                                                                                             cctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                                                  <  1:610940‑M2/177‑1 (MQ=255)                                                                                                                                                     acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAAtt                                                                                                                                                         <  1:658073‑M2/122‑1 (MQ=255)                                                                                                                                                     acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAat                                                                                                             <  1:181142‑M2/166‑1 (MQ=255)                                                                                                                                                     acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGAt                                                                                                        >  1:349224‑M2/23‑193 (MQ=255)                                                                                                                                                     acgtataaccatatatggctgtT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                                           <  1:445310‑M2/168‑1 (MQ=255)                                                                                                                                                       gtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGc                                                                                       <  1:482943‑M2/188‑1 (MQ=255)                                                                                                                                                            accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATaa                                                                                                                                                           <  1:383742‑M2/120‑1 (MQ=255)                                                                                                                                                            accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTc                                                                                                                <  1:780432‑M2/163‑1 (MQ=255)                                                                                                                                                            accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtg                                                                  <  1:637499‑M2/209‑1 (MQ=255)                                                                                                                                                            accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGccc                                                           <  1:781562‑M2/216‑1 (MQ=255)                                                                                                                                                            accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGttt                                        >  1:591276‑M2/16‑250 (MQ=255)                                                                                                                                                             ccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                                                  <  1:621619‑M2/177‑1 (MQ=255)                                                                                                                                                             ccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGttt                                        >  1:411858‑M2/15‑249 (MQ=255)                                                                                                                                                               atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAat                                                                                                             >  1:336420‑M2/13‑178 (MQ=255)                                                                                                                                                               atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAAc                                                                                   <  1:497351‑M2/192‑1 (MQ=255)                                                                                                                                                               atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                                      <  1:348005‑M2/205‑1 (MQ=255)                                                                                                                                                                tatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctctt                                   >  1:139343‑M2/12‑251 (MQ=255)                                                                                                                                                                 atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                                      >  1:364300‑M2/11‑215 (MQ=255)                                                                                                                                                                 atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCAccacca                                                                    <  1:192644‑M2/207‑1 (MQ=255)                                                                                                                                                                 atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttg                                  <  1:653899‑M2/241‑1 (MQ=255)                                                                                                                                                                           ttaaTGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctcttgagc                         <  1:606047/249‑4 (MQ=255)                                                                                                                                                                            taaTGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctcttgagcc                        <  1:148120/250‑5 (MQ=255)                                                                                                                                                                                  caaCTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGccc                                                           >  1:645615/3‑210 (MQ=255)                                                                                                                                                                                  caaCTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctc                               >  1:817617/3‑238 (MQ=255)                                                                                                                                                                                         aGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                                      >  1:236684/1‑192 (MQ=255)                                                                                                                                                                                           cAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCcaccac          <  1:143813/250‑1 (MQ=255)                                                                                                                                                                                                 tGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACga   >  1:9290/1‑250 (MQ=255)                                                                                                                                                                                                   cccGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGAGCCTCGCGCTCCACCACTTGACgac  <  1:542021/250‑3 (MQ=255)                                                                                                                                                                                                     cGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctc                               >  1:353159/1‑219 (MQ=255)                                                                                                                                                                                                           aCCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGAt                                                                                                        >  1:172796/1‑140 (MQ=255)                                                                                                                                                                                                              ctgtttttCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                                      <  1:169208/167‑1 (MQ=255)                                                                                                                                                                                                                    ttCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctcttgagc                         >  1:808703/1‑207 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      CGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCTTCGCGCTCCACCACTTGACGGC  >  1/1476711‑1477151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑