Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,887	G→A	intergenic (‑105/+65)	unknown ← / ← unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,887	0	G	A	100.0%	163.2 / NA	62	intergenic (‑105/+65)	unknown/unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/33);  total (29/33)

TCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCT  >  1/1476667‑1477129                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAACCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGaa                                                                                                                                                                                                              >  1:710923/1‑251 (MQ=255) tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAAAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTTCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCCGTTTTTc                                                                                                                                                                                                                  >  1:143318/1‑247 (MQ=255)                     ggaccGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacac                                                                                                                                                                                                 >  1:396556/5‑239 (MQ=255)                                              cGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATa                                                                                                                                                                  <  1:711569/251‑1 (MQ=255)                                                     ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTT‑AGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGCCTTTTTCTTACTTCTCGCTCAGATACGGATc                                                                                                                                                            >  1:43973/1‑250 (MQ=255)                                                     ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGAccc                                                                                                                                                                                                                          >  1:330856/1‑188 (MQ=255)                                                     ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAATAAGTATTTTTTTTACATGATATTCC‑TTCAGGTTGAAAAAAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAA‑‑‑‑ACAT‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGGTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTTCGACTTTTTTTTACTTCTCGCTCAGATACGGATCg                                                                                                                                                           >  1:386685/1‑251 (MQ=255)                                                      gataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                             <  1:444596/248‑1 (MQ=255)                                                            aaccggcacAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGGATTTTTTTTACATGATATTTC‑TTCAGGGTGGAAATAGATTAATTATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAaag                                                                                                                                                     >  1:189979/9‑250 (MQ=255)                                                            aaccggcacAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                             >  1:580545/9‑242 (MQ=255)                                                                          gTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacac                                                                                                                                                                                                 >  1:771184/1‑186 (MQ=255)                                                                                        cGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTCTCCGACTTTTTCTTACTTCTCGCTCAGATAc                                                                                                                                                                 <  1:42256/210‑1 (MQ=255)                                                                                                             gtaagtatttttttaacatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCAttt                                                                                                             >  1:187543‑M2/108‑251 (MQ=255)                                                                                                                 gtatttttttaacatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTtcct                                                                                                          >  1:702070‑M2/104‑250 (MQ=255)                                                                                                                         atTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacactttc                                                                                                                                                                                             >  1:424091/1‑139 (MQ=255)                                                                                                                            acatgatattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCAt                                                                                              >  1:842769‑M2/93‑251 (MQ=255)                                                                                                                             ccttcaGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                             >  1:391927/6‑178 (MQ=255)                                                                                                                                 atattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTtcc                                                                                                           >  1:333155‑M2/88‑233 (MQ=255)                                                                                                                                 atattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCa                                                                                               <  1:204574‑M2/158‑1 (MQ=255)                                                                                                                                  tattccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCaa                                                                                        >  1:426788‑M2/87‑251 (MQ=255)                                                                                                                                     tccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                     <  1:690648‑M2/168‑1 (MQ=255)                                                                                                                                     tccttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                     <  1:722156‑M2/168‑1 (MQ=255)                                                                                                                                        ttcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGAt                                                                                  <  1:446602‑M2/171‑1 (MQ=255)                                                                                                                                         tcaggttgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATc                                                                                 <  1:471959‑M2/172‑1 (MQ=255)                                                                                                                                             aGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                                                                             >  1:460939/1‑162 (MQ=255)                                                                                                                                               tgaaaatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAcc                                                                           <  1:472576‑M2/178‑1 (MQ=255)                                                                                                                                                   aatagattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGt                                                                       <  1:66273‑M2/182‑1 (MQ=255)                                                                                                                                                       gattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                            >  1:855640‑M2/66‑242 (MQ=255)                                                                                                                                                       gattaatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAAt                                                                   >  1:761325‑M2/66‑251 (MQ=255)                                                                                                                                                          atccggtcccttccgtgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAaccg                                                                          <  1:333148‑M2/179‑2 (MQ=255)                                                                                                                                                           aatcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCaa                                                               >  1:845801‑M2/62‑251 (MQ=255)                                                                                                                                                            atcattgtcccgctgcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                            <  1:150714‑M2/177‑1 (MQ=255)                                                                                                                                                                 gtcccgctgcagcaaccttaaaagcctgaattacggtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGc                                                          >  1:618874‑M2/56‑250 (MQ=255)                                                                                                                                                                          gcagcaaccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                            >  1:127641‑M2/47‑223 (MQ=255)                                                                                                                                                                                accttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgtg                                          <  1:115923‑M2/211‑1 (MQ=255)                                                                                                                                                                                accttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgtg                                          <  1:797600‑M2/211‑1 (MQ=255)                                                                                                                                                                                 ccttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATaa                                                                                                                                     <  1:366629‑M2/120‑1 (MQ=255)                                                                                                                                                                                  cttaaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                <  1:897391‑M2/205‑1 (MQ=255)                                                                                                                                                                                     aaaagcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGccc                                     <  1:530022‑M2/216‑1 (MQ=255)                                                                                                                                                                                        agcctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtgt                                           >  1:246222‑M2/33‑242 (MQ=255)                                                                                                                                                                                          cctgaattacgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                            <  1:610940‑M2/177‑1 (MQ=255)                                                                                                                                                                                                  acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAAtt                                                                                                                                   <  1:658073‑M2/122‑1 (MQ=255)                                                                                                                                                                                                  acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAat                                                                                       <  1:181142‑M2/166‑1 (MQ=255)                                                                                                                                                                                                  acgtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGAt                                                                                  >  1:349224‑M2/23‑193 (MQ=255)                                                                                                                                                                                                  acgtataaccatatatggctgtT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAatat                                                                                     <  1:445310‑M2/168‑1 (MQ=255)                                                                                                                                                                                                    gtataaccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGc                                                                 <  1:482943‑M2/188‑1 (MQ=255)                                                                                                                                                                                                         accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATaa                                                                                                                                     <  1:383742‑M2/120‑1 (MQ=255)                                                                                                                                                                                                         accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTc                                                                                          <  1:780432‑M2/163‑1 (MQ=255)                                                                                                                                                                                                         accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCAtg                                            <  1:637499‑M2/209‑1 (MQ=255)                                                                                                                                                                                                         accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGccc                                     <  1:781562‑M2/216‑1 (MQ=255)                                                                                                                                                                                                         accatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGttt                  >  1:591276‑M2/16‑250 (MQ=255)                                                                                                                                                                                                          ccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAAc                                                                            <  1:621619‑M2/177‑1 (MQ=255)                                                                                                                                                                                                          ccatatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGttt                  >  1:411858‑M2/15‑249 (MQ=255)                                                                                                                                                                                                            atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAat                                                                                       >  1:336420‑M2/13‑178 (MQ=255)                                                                                                                                                                                                            atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAAc                                                             <  1:497351‑M2/192‑1 (MQ=255)                                                                                                                                                                                                            atatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                <  1:348005‑M2/205‑1 (MQ=255)                                                                                                                                                                                                             tatatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctctt             >  1:139343‑M2/12‑251 (MQ=255)                                                                                                                                                                                                              atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGcaccac                                                >  1:364300‑M2/11‑215 (MQ=255)                                                                                                                                                                                                              atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCAccacca                                              <  1:192644‑M2/207‑1 (MQ=255)                                                                                                                                                                                                              atatggctttT‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttg            <  1:653899‑M2/241‑1 (MQ=255)                                                                                                                                                                                                                        ttaaTGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctcttgagc   <  1:606047/249‑4 (MQ=255)                                                                                                                                                                                                                         taaTGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAAGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCGAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTctcttgctcttgagcc  <  1:148120/250‑5 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCACCACCATGTGTGCCCAATGCGCCATGAGCGGTTTCTCTTGCTCTTGGGCT  >  1/1476667‑1477129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑