Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,842	C→T	intergenic (‑60/+110)	unknown ← / ← unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,842	0	C	T	100.0%	118.0 / NA	46	intergenic (‑60/+110)	unknown/unknown	hypothetical protein/Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

ATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTAC‑GTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCA  >  1/1476602‑1477079                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                aTCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑gt                                                                                                                                                                                                                                       <  1:215132/251‑1 (MQ=255)       gATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAAc                                                                                                                                                                                                                                  <  1:357354/250‑1 (MQ=255)           cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACcatat                                                                                                                                                                                                                             <  1:693307/251‑1 (MQ=255)           cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACcatat                                                                                                                                                                                                                             <  1:231242/251‑1 (MQ=255)            tGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACcatata                                                                                                                                                                                                                            <  1:135765/251‑1 (MQ=255)                                                                catCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAAAAGATTAATCATTTTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCatatat                                                                                                                                                                                                                           >  1:762781/3‑200 (MQ=255)                                                                  tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACcatat                                                                                                                                                                                                                             <  1:819786/196‑1 (MQ=255)                                                                  tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggc                                                                                                                                                                                                                        <  1:593899/201‑3 (MQ=255)                                                                  tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAACCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGaa                                                                                                                                                            >  1:710923/1‑251 (MQ=255)                                                                  tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAAAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTTCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCCGTTTTTc                                                                                                                                                                >  1:143318/1‑247 (MQ=255)                                                                                      ggaccGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacac                                                                                                                                               >  1:396556/5‑239 (MQ=255)                                                                                                               cGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATa                                                                                                                <  1:711569/251‑1 (MQ=255)                                                                                                                      ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTT‑AGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGCCTTTTTCTTACTTCTCGCTCAGATACGGATc                                                                                                          >  1:43973/1‑250 (MQ=255)                                                                                                                      ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGAccc                                                                                                                                                                        >  1:330856/1‑188 (MQ=255)                                                                                                                      ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAATAAGTATTTTTTTTACATGATATTCC‑TTCAGGTTGAAAAAAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAA‑‑‑‑ACAT‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGGTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTTCGACTTTTTTTTACTTCTCGCTCAGATACGGATCg                                                                                                         >  1:386685/1‑251 (MQ=255)                                                                                                                       gataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                           <  1:444596/248‑1 (MQ=255)                                                                                                                             aaccggcacAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGGATTTTTTTTACATGATATTTC‑TTCAGGGTGGAAATAGATTAATTATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGATCGACAaag                                                                                                   >  1:189979/9‑250 (MQ=255)                                                                                                                             aaccggcacAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                           >  1:580545/9‑242 (MQ=255)                                                                                                                                           gTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacac                                                                                                                                               >  1:771184/1‑186 (MQ=255)                                                                                                                                                         cGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTCTCCGACTTTTTCTTACTTCTCGCTCAGATAc                                                                                                               <  1:42256/210‑1 (MQ=255)                                                                                                                                                                    gtaagtaTTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccattt                                                                     >  1:187543‑M1/1‑97 (MQ=255)                                                                                                                                                                        gtaTTTTTTTAACATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcct                                                                  >  1:702070‑M1/1‑93 (MQ=255)                                                                                                                                                                                   aCATGATATTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcat                                                      >  1:842769‑M1/1‑82 (MQ=255)                                                                                                                                                                                        atatTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtca                                                       <  1:204574‑M1/245‑169 (MQ=255)                                                                                                                                                                                        atatTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcc                                                                   >  1:333155‑M1/1‑77 (MQ=255)                                                                                                                                                                                         tatTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaa                                                >  1:426788‑M1/1‑76 (MQ=255)                                                                                                                                                                                          atTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacactttc                                                                                                                                           >  1:424091/1‑139 (MQ=255)                                                                                                                                                                                             tccttcaggttgaAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatat                                             <  1:722156‑M1/245‑179 (MQ=255)                                                                                                                                                                                             tccttcaggttgaAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatat                                             <  1:690648‑M1/245‑179 (MQ=255)                                                                                                                                                                                              ccttcaGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                           >  1:391927/6‑178 (MQ=255)                                                                                                                                                                                                ttcaggtTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgat                                          <  1:446602‑M1/248‑182 (MQ=255)                                                                                                                                                                                                 tcaggTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatc                                         <  1:471959‑M1/249‑183 (MQ=255)                                                                                                                                                                                                       tGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataacc                                   <  1:472576‑M1/251‑189 (MQ=255)                                                                                                                                                                                                           aaTAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagt                               <  1:66273‑M1/251‑193 (MQ=255)                                                                                                                                                                                                              aGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt                                                                                                           >  1:460939/1‑162 (MQ=255)                                                                                                                                                                                                               gattaatcatTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaat                           >  1:761325‑M1/5‑55 (MQ=255)                                                                                                                                                                                                               gattaatcatTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataac                                    >  1:855640‑M1/5‑55 (MQ=255)                                                                                                                                                                                                                  atccggtcccttccgtgcagcaaccttaaaaGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccg                                  <  1:333148‑M1/226‑190 (MQ=255)                                                                                                                                                                                                                   aaTCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaatgcaa                       >  1:845801‑M1/1‑51 (MQ=255)                                                                                                                                                                                                                    aTCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataac                                    <  1:150714‑M1/237‑188 (MQ=255)                                                                                                                                                                                                                          gtcccgctgCAGCAACCTTAAAAGCCTGAATTACGGTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaatgcaaactgc                  >  1:618874‑M1/5‑45 (MQ=255)                                                                                                                                                                                                                                  gcagcaACCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataac                                    >  1:127641‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                                        aCCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaatgcaaactgccgtgcaccaccatgtg  <  1:797600‑M1/251‑222 (MQ=255)                                                                                                                                                                                                                                        aCCTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaatgcaaactgccgtgcaccaccatgtg  <  1:115923‑M1/251‑222 (MQ=255)                                                                                                                                                                                                                                         ccTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataa                                                                                             <  1:366629‑M1/159‑131 (MQ=255)                                                                                                                                                                                                                                          cTTAAAAGCCTGAATTAC‑GTATAACCATatatggcttttaatggcaactgaagcaagaatgcccgtttgaccctgtttttccgaaatagttttacactttccgactttttcttacttctcgctcagatacggatcgacaaagccaagccctatcataatttgcgtttccaagctttccatttcctccgcttcgtcatcttcaatatgatcataaccgagtaaatgcaaactgccgtgcaccac        <  1:897391‑M1/243‑216 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                ATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTAC‑GTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCGACAAAGCCAAGCCCTATCATAATTTGCGTTTCCAGGCTTTCCATTTCCTCCGCTTCGTCATCTTCAATATGATCATAACCAAGTAAATGCAAACTGCCGTGCA  >  1/1476602‑1477079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑