Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,732	G→A	C17C (TGC→TGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,732	0	G	A	100.0%	98.5 / NA	36	C17C (TGC→TGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/26);  total (10/26)

TTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCG  >  1/1476488‑1476976                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         tttCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                                                                                                                                                    <  1:107284/251‑1 (MQ=255)          cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                                                                                                                                            <  1:421049/248‑1 (MQ=255)          cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                                                                                                                                            <  1:335878/248‑1 (MQ=255)          cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAAACAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                                                                                                                                                    >  1:804429/3‑242 (MQ=255)           aatAACACGTTCGTCCCATTTCTTTCCCAATTCCCGCAACCAACAAAATTCCAAGTTTTTTTTTGCTTAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                                                                                                                                          <  1:157874/249‑1 (MQ=255)            gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGCTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTtaaaaaaa                                                                                                                                                                                                                                         >  1:783462/1‑247 (MQ=255)                        tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                                                                                                                                             <  1:126569/251‑2 (MQ=255)                        tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                                                                                                                                             <  1:267454/251‑2 (MQ=255)                             ttgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagta                                                                                                                                                                                                                        <  1:664378/251‑1 (MQ=255)                              tgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                                                                                                                                          <  1:162855/232‑1 (MQ=255)                                cttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAAtaagtaag                                                                                                                                                                                                                      <  1:586646/250‑1 (MQ=255)                                   gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                                                                                                                                          <  1:157371/227‑1 (MQ=255)                                                     acaaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTcc                                                                                                                                                                                               <  1:417865/251‑1 (MQ=255)                                                      caaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaag                                                                                                                                                                                                                          <  1:318695/224‑1 (MQ=255)                                                        atatTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                                                                                                                                          <  1:161632/206‑1 (MQ=255)                                                                                    aaTTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTgtcc                                                                                                                                                                <  1:23965/250‑4 (MQ=255)                                                                                      tttttCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTgtcccgc                                                                                                                                                             <  1:772262/251‑7 (MQ=255)                                                                                                                   aTCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACgt                                                                                                                                <  1:215132/251‑1 (MQ=255)                                                                                                                         gATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAAc                                                                                                                           <  1:357354/250‑1 (MQ=255)                                                                                                                             cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat                                                                                                                      <  1:231242/251‑1 (MQ=255)                                                                                                                             cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat                                                                                                                      <  1:693307/251‑1 (MQ=255)                                                                                                                              tGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatata                                                                                                                     <  1:135765/251‑1 (MQ=255)                                                                                                                                                              gAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagtaagt                                                                                                                                                                                                                     <  1:673166/125‑1 (MQ=255)                                                                                                                                                                                  catCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAAAAGATTAATCATTTTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCatatat                                                                                                                    >  1:762781/3‑200 (MQ=255)                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat                                                                                                                      <  1:819786/196‑1 (MQ=255)                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCATatatggc                                                                                                                 <  1:593899/201‑3 (MQ=255)                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGGAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAACCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGaa                                                     >  1:710923/1‑251 (MQ=255)                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAAAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGGTGAAAATAGATTAATCATTGTTCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCCGTTTTTc                                                         >  1:143318/1‑247 (MQ=255)                                                                                                                                                                                    tCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAAAGAAAACGGTAAAATAAGTAAGTATTTTTTTAACATGATATTTCT‑TT‑AGGTTGAAAAAAGATTAATCATTGTCCCGCTGCAGCAACCTTaaaaacctgaattaagt                                                                                                                                >  1:840948/1‑173 (MQ=255)                                                                                                                                                                                         aTGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAAt                                                                                                                                                                                <  1:409835/133‑1 (MQ=255)                                                                                                                                                                                                        ggaccGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAG‑ttttacac                                        >  1:396556/5‑239 (MQ=255)                                                                                                                                                                                                                                 cGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATa         <  1:711569/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCCTTT‑AGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAAAGCCCGTTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTCCGCCTTTTTCTTACTTCTCGCTCAGATACGGATc   >  1:43973/1‑250 (MQ=255)                                                                                                                                                                                                                                        ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGAccc                                                                 >  1:330856/1‑188 (MQ=255)                                                                                                                                                                                                                                        ggataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAATAAGTATTTTTTTTACATGATA‑TTCC‑TTCAGGTTGAAAAAAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAA‑‑‑‑ACAT‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGGTTGACCCTGTTTTTTCGAAATAGTTTTACACTTTTCGACTTTTTTTTACTTCTCGCTCAGATACGGATCg  >  1:386685/1‑251 (MQ=255)                                                                                                                                                                                                                                         gataaTAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TTCAGGGTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACCAT‑‑‑‑‑ATATGGCTTT‑‑‑‑T‑AATGGCAACTGAAGCAAGAATGCCCGTTTGACCCTGTTTTTCCGAAATAGTTTTACACTTTCCGACTTTTTCTTACTTCTCGCTCAGATACGGAt    <  1:444596/248‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         TTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATA‑TTCC‑TT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATATGATATGGCTTTAATATAAATGGCGACTGAAGCAAGAATGCCCGTTTGACCCTGATTTTCCGAAATAG‑TTTACGCATTCCGAATTTTTCTTACTTCTCGCTCAGATACGGATCG  >  1/1476488‑1476976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑