Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,666	G→A	D39D (GAC→GAT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,666	0	G	A	100.0%	162.4 / NA	60	D39D (GAC→GAT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/45);  total (15/45)

TACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCCTT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATA  >  1/1476422‑1476862                                                                                                                                                                                                                                                     |                                                                                                                                                                                                       tgcgcgCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTa                                                                                                                                                                                                  <  1:344240/249‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:485292/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:359877/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:14263/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:590613/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:311624/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:841660/250‑1 (MQ=255)     cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:663833/250‑1 (MQ=255)       ccgatggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:877428/241‑1 (MQ=255)           tggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACgg                                                                                                                                                                                        >  1:33720/4‑251 (MQ=255)             gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                                                                                                                      >  1:689318/2‑251 (MQ=255)             gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                                                                                                                      >  1:99062/2‑251 (MQ=255)              tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                                                                                                                     <  1:591671/251‑2 (MQ=255)              tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                                                                                                                     <  1:585668/251‑2 (MQ=255)                tGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaaggg                                                                                                                                                                                   <  1:803649/251‑4 (MQ=255)                   ccACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc                                                                                                                                                                                <  1:735009/251‑7 (MQ=255)                        aTCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               >  1:760806/1‑231 (MQ=255)                           ttCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                                                                                                                        <  1:13011/251‑15 (MQ=255)                            tcgccAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                                                                                                                       <  1:810757/247‑16 (MQ=255)                                  aCTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTTCTGAAAACGGCCTTTTCTTCATCATTATTATGCTGCACGGaagggggcgga                                                                                                                                                                             >  1:677864/1‑230 (MQ=255)                                     cGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccaggttcaaat                                                                                                                                                              <  1:895388/251‑25 (MQ=255)                                            gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                                                                                                                        <  1:155253/234‑15 (MQ=255)                                            gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGGTCAAATTGCggat                                                                                                                                                      >  1:802080/1‑251 (MQ=255)                                                  atCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataaggat                                                                                                                                                >  1:474733/1‑251 (MQ=255)                                                   tCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                                                                                                                       <  1:841763/228‑16 (MQ=255)                                                     ttttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataat                                                                                                                                             <  1:136211/251‑1 (MQ=255)                                                      tttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGGTAACCAGGGTCAAATTGCGGATAAGGataata                                                                                                                                            >  1:585978/1‑251 (MQ=255)                                                       ttaataagcGTTTTTCAAGGGCAATAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                                                                                                                           <  1:412543/243‑1 (MQ=255)                                                        taataagcGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                                                                                                                           >  1:521803/8‑250 (MQ=255)                                                                   tttCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                               <  1:107284/251‑1 (MQ=255)                                                                            cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccag                                                                                                                                                                      >  1:100859/3‑188 (MQ=255)                                                                            cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                       <  1:421049/248‑1 (MQ=255)                                                                            cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                       <  1:335878/248‑1 (MQ=255)                                                                            cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAAACAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                               >  1:804429/3‑242 (MQ=255)                                                                             aatAACACGTTCGTCCCATTTCTTTCCCAATTCCCGCAACCAACAAAATTCCAAGTTTTTTTTTGCTTAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:157874/249‑1 (MQ=255)                                                                              gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCAc                                                                                                                                                                                          <  1:778236/182‑1 (MQ=255)                                                                              gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataata                                                                                                                                            >  1:248727/1‑227 (MQ=255)                                                                              gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGCTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTtaaaaaaa                                                                                                                    >  1:783462/1‑247 (MQ=255)                                                                                          tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:417308/165‑1 (MQ=255)                                                                                          tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                        <  1:126569/251‑2 (MQ=255)                                                                                          tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                        <  1:267454/251‑2 (MQ=255)                                                                                               ttgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagta                                                                                                   <  1:664378/251‑1 (MQ=255)                                                                                                tgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:162855/232‑1 (MQ=255)                                                                                                  cttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAAtaagtaag                                                                                                 <  1:586646/250‑1 (MQ=255)                                                                                                     gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:409028/154‑1 (MQ=255)                                                                                                     gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataagga                                                                                                                                                 >  1:312469/1‑199 (MQ=255)                                                                                                     gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:157371/227‑1 (MQ=255)                                                                                                                       acaaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTcc                                                                           <  1:417865/251‑1 (MQ=255)                                                                                                                        caaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc                                                                                                                                                                                <  1:524781/150‑7 (MQ=255)                                                                                                                        caaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaag                                                                                                     <  1:318695/224‑1 (MQ=255)                                                                                                                          atatTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:161632/206‑1 (MQ=255)                                                                                                                                                      aaTTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTgtcc                                             <  1:23965/250‑4 (MQ=255)                                                                                                                                                        tttttCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTgtcccgc                                          <  1:772262/251‑7 (MQ=255)                                                                                                                                                                                     aTCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACgt             <  1:215132/251‑1 (MQ=255)                                                                                                                                                                                           gATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAAc        <  1:357354/250‑1 (MQ=255)                                                                                                                                                                                               cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat   <  1:231242/251‑1 (MQ=255)                                                                                                                                                                                               cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat   <  1:693307/251‑1 (MQ=255)                                                                                                                                                                                                tGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatata  <  1:135765/251‑1 (MQ=255)                                                                                                                                                                                                 gATATTTCGGTTCATGCGTGCGGCGGGCGCTGAAAACGGCATTTTCTTCATCATCATTATTCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataagga                                                                                                                                                 >  1:185424/1‑107 (MQ=255)                                                                                                                                                                                                                                gAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagtaagt                                                                                                <  1:673166/125‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                       TACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCCTT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATA  >  1/1476422‑1476862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑